(Circular) (Six-base) MAPSORT of: pFN21AE5338.seq Check: 3217 from: 1 to: 5497 pFN21AE5338 5497 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2448 3307 278 Size: 53 83 186 936 912 859 2468 Fragments arranged by size: 2468 936 912 859 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2360 2818 2360 Size: 458 5039 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3560 4944 5212 1632 Size: 1928 1384 268 1917 Fragments arranged by size: 1928 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 4396 5373 1203 Size: 3193 977 1327 Fragments arranged by size: 3193 1327 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2534 1184 Size: 1350 4147 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 2375 Size: 32 5465 AlwNI CAG_nnn'CTG Cuts at: 2324 3240 4812 2324 Size: 916 1572 3009 Fragments arranged by size: 3009 1572 916 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2806 3032 3184 5293 1493 Size: 1313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2628 2822 1870 Size: 73 685 194 4545 Fragments arranged by size: 4545 685 194 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 2360 2497 2818 4273 Size: 325 217 627 228 345 137 321 1455 Cuts at: 4273 618 Size: 1842 Fragments arranged by size: 1842 1455 627 345 325 321 228 217 137 BanII G_rGCy'C Cuts at: 1391 2816 1391 Size: 1425 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2626 3389 4599 2626 Size: 763 1210 3524 Fragments arranged by size: 3524 1210 763 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4108 136 Size: 107 122 71 631 605 2436 1525 Fragments arranged by size: 2436 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2018 2169 2691 3558 4714 1790 Size: 228 151 522 867 1156 2573 Fragments arranged by size: 2573 1156 867 522 228 151 BmgBI CAC'GTC Cuts at: 1318 2380 1318 Size: 1062 4435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2014 2476 3228 Size: 416 53 83 399 24 597 462 752 Cuts at: 3228 4186 442 Size: 958 1753 Fragments arranged by size: 1753 958 752 597 462 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4157 1239 Size: 2918 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3622 4487 4785 3622 Size: 865 298 4334 Fragments arranged by size: 4334 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 3304 3686 4321 Size: 53 83 186 936 912 859 382 635 Cuts at: 4321 275 Size: 1451 Fragments arranged by size: 1451 936 912 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3930 4328 4602 4749 5160 3930 Size: 398 274 147 411 4267 Fragments arranged by size: 4267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2008 2038 2375 2405 1178 Size: 30 165 30 605 30 337 30 4270 Fragments arranged by size: 4270 605 337 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4647 BseYI C'CCAG_C Cuts at: 2168 2213 2492 2505 2767 4700 5196 2168 Size: 45 279 13 262 1933 496 2469 Fragments arranged by size: 2469 1933 496 279 262 45 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4924 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2707 2947 3708 4736 1540 Size: 275 186 706 240 761 1028 2301 Fragments arranged by size: 2301 1028 761 706 275 240 186 BsiHKAI G_wGCw'C Cuts at: 3558 3643 4714 3558 Size: 85 1071 4341 Fragments arranged by size: 4341 1071 85 BsmI GAATG_Cn' Cuts at: 2191 3008 3101 2191 Size: 817 93 4587 Fragments arranged by size: 4587 817 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2169 2691 2816 3558 3643 4714 Size: 399 228 151 522 125 742 85 1071 Cuts at: 4714 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 522 399 228 151 125 85 BspEI T'CCGG_A Cuts at: 5160 5160 Size: 5497 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2723 2852 844 Size: 1879 129 3489 Fragments arranged by size: 3489 1879 129 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2565 3992 4166 65 Size: 1183 1317 1427 174 1396 Fragments arranged by size: 1427 1396 1317 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4141 4328 1700 Size: 110 2331 187 2869 Fragments arranged by size: 2869 2331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstXI CCAn_nnnn'nTGG Cuts at: 2675 2675 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2209 2321 2827 3194 3579 3596 5048 5134 Size: 237 112 506 367 385 17 1452 86 Cuts at: 5134 5146 5492 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 506 385 367 346 237 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3336 Fragments arranged by size: 3336 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2497 487 Size: 683 56 1271 3487 Fragments arranged by size: 3487 1271 683 56 BtsI GCAGTG_nn' Cuts at: 2778 3013 3807 3827 2778 Size: 235 794 20 4448 Fragments arranged by size: 4448 794 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DraIII CAC_nnn'GTG Cuts at: 2380 2380 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2550 2944 3833 Size: 54 1425 50 275 18 720 394 889 Cuts at: 3833 5462 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 720 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3560 4157 4944 5212 1239 Size: 393 1928 597 787 268 1524 Fragments arranged by size: 1928 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 2683 4644 1678 Size: 1005 1961 2531 Fragments arranged by size: 2531 1961 1005 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373 Size: 32 135 32 2566 32 42 32 2626 Fragments arranged by size: 2626 2566 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 3087 669 Size: 988 281 903 246 3079 Fragments arranged by size: 3079 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KpnI G_GTAC'C Cuts at: 2364 2822 2364 Size: 458 5039 MfeI C'AATT_G Cuts at: 2336 3096 2336 Size: 760 4737 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4611 4795 1305 Size: 3306 184 2007 Fragments arranged by size: 3306 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2552 10 Size: 54 1425 343 720 2955 Fragments arranged by size: 2955 1425 720 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2673 3455 3814 3973 518 Size: 648 1507 782 359 159 2042 Fragments arranged by size: 2042 1507 782 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2611 2913 3590 4738 4983 1606 Size: 1005 302 677 1148 245 2120 Fragments arranged by size: 2120 1148 1005 677 302 245 NcoI C'CATG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3336 Fragments arranged by size: 3336 1609 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 1108 Size: 1272 4225 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2375 2407 3566 3598 5079 5111 2375 Size: 32 1159 32 1481 32 2761 Fragments arranged by size: 2761 1481 1159 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 2274 3067 2274 Size: 793 4704 PstI C_TGCA'G Cuts at: 830 2241 2734 2849 830 Size: 1411 493 115 3478 Fragments arranged by size: 3478 1411 493 115 PvuI CG_AT'CG Cuts at: 2001 2707 2001 Size: 706 4791 PvuII CAG'CTG Cuts at: 2611 2611 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2237 2730 2845 3980 4661 4852 826 Size: 219 1192 493 115 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 493 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2630 2824 1872 Size: 758 194 4545 Fragments arranged by size: 4545 758 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 3601 4502 4764 820 Size: 197 926 1658 901 262 1553 Fragments arranged by size: 1658 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 2305 3421 5 Size: 47 2253 1116 2081 Fragments arranged by size: 2253 2081 1116 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2571 2674 2936 513 Size: 552 1506 103 262 3074 Fragments arranged by size: 3074 1506 552 262 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2033 2687 3524 3541 3694 3879 4324 Size: 1255 453 654 837 17 153 185 445 Cuts at: 4324 325 Size: 1498 Fragments arranged by size: 1498 1255 837 654 453 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2008 2040 2313 2345 5457 5489 2008 Size: 32 273 32 3112 32 2016 Fragments arranged by size: 3112 2016 273 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2489 2489 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2628 2822 1870 Size: 758 194 4545 Fragments arranged by size: 4545 758 194 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2446 3305 276 Size: 53 83 186 936 912 859 2468 Fragments arranged by size: 2468 936 912 859 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI