Restriction map for
pFN21AE6550
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstYI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI Ksp632I KspI MaeII MamI
MboII MfeI MflI MluI MroI
MseI MspA1I MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 304 CGCG CG^CG
AcyI 295 GACGCC G[GA]^CG[CT]C
AlwI 126 GATCC GGATC(4/5)
AocI 54 CCTCAGG CC^T[ACGT]AGG
AvaI 332 CCCGAG C^[CT]CG[GA]G
AvaII 33 GGTCC G^G[AT]CC
BanII 132 GGGCTC G[GA]GC[CT]^C
BfaI 121 CTAG C^TAG
BpmI 172 CTGGAG CTGGAG(16/14)
BsaHI 295 GACGCC G[GA]^CG[CT]C
BseRI 52 CTCCTC GAGGAG(10/8)
BsmFI 117 GGGAC GGGAC(10/14)
BsoBI 332 CCCGAG C^[CT]CG[GA]G
BspHI 282 TCATGA T^CATGA
BsrDI 65 CATTGC GCAATG(2/0)
BsrFI 235 GCCGGC [GA]^CCGG[CT]
BstUI 304 CGCG CG^CG
BstXI 137 CCACCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 54 CCTCAGG CC^T[ACGT]AGG
Cfr10I 235 GCCGGC [GA]^CCGG[CT]
DpnII 126 GATC ^GATC
Eco81I 54 CCTCAGG CC^T[ACGT]AGG
EcoT14I 164 CCTTGG C^C[AT][AT]GG
HapII 236 CCGG C^CGG
HgaI 295 GACGC GACGC(5/10)
Hin1I 295 GACGCC G[GA]^CG[CT]C
HpaII 236 CCGG C^CGG
MaeI 121 CTAG C^TAG
MaeIII 85 GTGAC ^GT[ACGT]AC
MboI 126 GATC ^GATC
MslI 138 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 236 CCGG C^CGG
MvnI 304 CGCG CG^CG
NaeI 235 GCCGGC GCC^GGC
NdeII 126 GATC ^GATC
NgoMIV 235 GCCGGC G^CCGGC
PstI 310 CTGCAG CTGCA^G
RcaI 282 TCATGA T^CATGA
Sau3AI 126 GATC ^GATC
SfcI 310 CTGCAG C^T[GA][CT]AG
StyI 164 CCTTGG C^C[AT][AT]GG
Tsp45I 85 GTGAC ^GT[GC]AC
Tsp509I 98 AATT ^AATT
XcmI 155 CCACGGGAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 176 AGCT AG^CT
308 AGCT
BalI 205 TGGCCA TGG^CCA
272 TGGCCA
BmyI 132 GGGCTC G[AGT]GC[ACT]^C
252 GGGCAC
Bsp1286I 132 GGGCTC G[AGT]GC[ACT]^C
252 GGGCAC
CfoI 103 GCGC GCG^C
360 GCGC
DdeI 55 CTCAG C^T[ACGT]AG
342 CTTAG
DsaI 8 CCGTGG C^C[GA][CT]GG
155 CCACGG
EaeI 205 TGGCCA [CT]^GGCC[GA]
272 TGGCCA
HhaI 103 GCGC GCG^C
360 GCGC
HinP1I 103 GCGC G^CGC
360 GCGC
MluNI 205 TGGCCA TGG^CCA
272 TGGCCA
MscI 205 TGGCCA TGG^CCA
272 TGGCCA
MwoI 254 GCACTGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
337 GCCGACTTAGC
NlaIII 90 CATG CATG^
283 CATG
NlaIV 133 GGCTCC GG[ACGT]^[ACGT]CC
160 GGAGCC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I EcoRII
FauI Fnu4HI HaeIII ItaI MnlI
MvaI Sau96I ScrFI TseI TspRI