Restriction map for
pFN21AE6188
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 459 GTAC GT^AC
AluI 430 AGCT AG^CT
AocI 87 CCTCAGG CC^T[ACGT]AGG
AspI 118 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 84 GGACC G^G[AT]CC
BamHI 200 GGATCC G^GATCC
BanI 362 GGTGCC G^G[CT][GA]CC
BbsI 49 GTCTTC GAAGAC(2/6)
BbvI 229 GCTGC GCAGC(8/12)
BcnI 107 CCCGG CC^[GC]GG
BpuAI 49 GTCTTC GAAGAC(2/6)
BsaBI 201 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 194 GAGACG CGTCTC(1/5)
BsrDI 333 CATTGC GCAATG(2/0)
BsrFI 73 GCCGGC [GA]^CCGG[CT]
Bsu36I 87 CCTCAGG CC^T[ACGT]AGG
CfoI 232 GCGC GCG^C
Cfr10I 73 GCCGGC [GA]^CCGG[CT]
Cfr13I 84 GGACC G^G[ACGT]CC
DsaI 146 CCACGG C^C[GA][CT]GG
Eco81I 87 CCTCAGG CC^T[ACGT]AGG
EcoT14I 57 CCTTGG C^C[AT][AT]GG
FauI 7 GCGGG CCCGC(4/6)
HaeIII 76 GGCC GG^CC
HhaI 232 GCGC GCG^C
HinP1I 232 GCGC G^CGC
MaeII 122 ACGT A^CGT
MaeIII 424 GTCAC ^GT[ACGT]AC
MamI 201 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 50 TCTTC GAAGA(8/7)
MslI 96 CATGCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 431 GCTTTATCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 73 GCCGGC GCC^GGC
NciI 107 CCCGG CC^[GC]GG
NgoMIV 73 GCCGGC G^CCGGC
PflFI 118 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 459 GTAC GT^AC
Sau96I 84 GGACC G^G[ACGT]CC
SphI 40 GCATGC GCATG^C
StyI 57 CCTTGG C^C[AT][AT]GG
TaqI 378 TCGA T^CGA
TseI 229 GCTGC G^C[AT]GC
Tsp45I 424 GTCAC ^GT[GC]AC
TspRI 221 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 118 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 378 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 127 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
238 CAGACCCTG
BsiYI 108 CCGGAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCCTCGGAAGG
BslI 108 CCGGAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCCTCGGAAGG
BsmAI 194 GAGAC GTCTC(1/5)
314 GTCTC
BsrI 485 ACTGG ACTGG(1/-1)
126 CCAGT
BstYI 200 GGATCC [GA]^GATC[CT]
387 AGATCC
DpnII 201 GATC ^GATC
388 GATC
Fnu4HI 11 GCGGC GC^[ACGT]GC
229 GCTGC
HapII 74 CCGG C^CGG
108 CCGG
HincII 154 GTCAAC GT[CT]^[GA]AC
214 GTCAAC
HindII 154 GTCAAC GT[CT]^[GA]AC
214 GTCAAC
HpaII 74 CCGG C^CGG
108 CCGG
ItaI 11 GCGGC GC^[ACGT]GC
229 GCTGC
MboI 201 GATC ^GATC
388 GATC
MflI 200 GGATCC [GA]^GATC[CT]
387 AGATCC
MspI 74 CCGG C^CGG
108 CCGG
NdeII 201 GATC ^GATC
388 GATC
PleI 22 GACTC GAGTC(4/5)
380 GACTC
Sau3AI 201 GATC ^GATC
388 GATC
TfiI 291 GATTC G^A[AT]TC
419 GAATC
XhoII 200 GGATCC [GA]^GATC[CT]
387 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AlwI BsaJI BssKI BstNI
Cac8I DdeI EcoRII HinfI HphI
MnlI MvaI NlaIII NlaIV NspI
ScrFI