(Circular) (Six-base) MAPSORT of: pFN21AE6188.seq Check: 969 from: 1 to: 5206 pFN21AE6188 5206 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3016 278 Size: 53 83 186 936 1480 2468 Fragments arranged by size: 2468 1480 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2549 1937 Size: 612 4594 Acc65I G'GTAC_C Cuts at: 2527 2527 Size: 5206 AclI AA'CG_TT Cuts at: 1563 3333 3706 1563 Size: 1770 373 3063 Fragments arranged by size: 3063 1770 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 3269 4653 4921 1632 Size: 593 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 593 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5009 AflIII A'CryG_T Cuts at: 1203 4105 5082 1203 Size: 2902 977 1327 Fragments arranged by size: 2902 1327 977 AgeI A'CCGG_T Cuts at: 4037 4037 Size: 5206 AhdI GACnn_n'nnGTC Cuts at: 3935 3935 Size: 5206 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5206 AlwNI CAG_nnn'CTG Cuts at: 2137 2248 2949 4521 2137 Size: 111 701 1572 2822 Fragments arranged by size: 2822 1572 701 111 ApaLI G'TGCA_C Cuts at: 3263 4419 3263 Size: 1156 4050 ApoI r'AATT_y Cuts at: 1493 2515 2741 2893 5002 1493 Size: 1022 226 152 2109 1697 Fragments arranged by size: 2109 1697 1022 226 152 AseI AT'TA_AT Cuts at: 160 1032 3760 160 Size: 872 2728 1606 Fragments arranged by size: 2728 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5206 AvaI C'yCGr_G Cuts at: 1870 1943 2531 1870 Size: 73 588 4545 Fragments arranged by size: 4545 588 73 BamHI G'GATC_C Cuts at: 2205 2536 2903 2205 Size: 331 367 4508 Fragments arranged by size: 4508 367 331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2367 2527 3982 618 Size: 325 217 627 580 160 1455 1842 Fragments arranged by size: 1842 1455 627 580 325 217 160 BanII G_rGCy'C Cuts at: 1391 2525 1391 Size: 1134 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2047 928 Size: 971 148 4087 Fragments arranged by size: 4087 971 148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3397 3431 1903 Size: 34 1460 34 3678 Fragments arranged by size: 3678 1460 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3098 4308 3098 Size: 1210 3996 BclI T'GATC_A Cuts at: 1548 1548 Size: 5206 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3817 136 Size: 107 122 71 631 605 337 1808 1525 Fragments arranged by size: 1808 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5201 5201 Size: 5206 BlpI GC'TnA_GC Cuts at: 2623 2623 Size: 5206 Bme1580I G_kGCm'C Cuts at: 1790 3267 4423 1790 Size: 1477 1156 2573 Fragments arranged by size: 2573 1477 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5206 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2937 3895 442 Size: 416 53 83 399 24 1520 958 1753 Fragments arranged by size: 1753 1520 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3866 1239 Size: 2627 2579 Bpu10I CC'TnA_GC Cuts at: 2289 2289 Size: 5206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3331 4196 4494 3331 Size: 865 298 4043 Fragments arranged by size: 4043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3869 882 Size: 617 2370 2219 Fragments arranged by size: 2370 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2997 493 Size: 711 1793 2702 Fragments arranged by size: 2702 1793 711 BsaBI GATnn'nnATC Cuts at: 2210 5200 2210 Size: 2990 2216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3013 3395 4030 275 Size: 53 83 186 936 1480 382 635 1451 Fragments arranged by size: 1480 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3639 4037 4311 4458 4869 3639 Size: 398 274 147 411 3976 Fragments arranged by size: 3976 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2034 2064 1178 Size: 30 165 30 631 30 4320 Fragments arranged by size: 4320 631 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5206 BseYI C'CCAG_C Cuts at: 4409 4905 4409 Size: 496 4710 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4633 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2656 3417 4445 1540 Size: 275 186 655 761 1028 2301 Fragments arranged by size: 2301 1028 761 655 275 186 BsiHKAI G_wGCw'C Cuts at: 3267 3352 4423 3267 Size: 85 1071 4050 Fragments arranged by size: 4050 1071 85 BsmI GAATG_Cn' Cuts at: 2717 2810 2717 Size: 93 5113 BsmBI CGTCTCn'nnnn_ Cuts at: 2193 2193 Size: 5206 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2525 3267 3352 4423 1391 Size: 399 735 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 399 85 BspEI T'CCGG_A Cuts at: 4869 4869 Size: 5206 BspHI T'CATG_A Cuts at: 3095 4825 3095 Size: 1730 3476 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 844 Size: 1717 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3093 1394 Size: 429 1270 3507 Fragments arranged by size: 3507 1270 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2337 3701 3875 65 Size: 1183 1089 1364 174 1396 Fragments arranged by size: 1396 1364 1183 1089 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 3850 4037 1700 Size: 110 268 1772 187 2869 Fragments arranged by size: 2869 1772 268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5206 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5206 BssSI C'ACGA_G Cuts at: 3266 4278 3266 Size: 1012 4194 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5206 BstBI TT'CG_AA Cuts at: 4010 4010 Size: 5206 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5206 BstYI r'GATC_y Cuts at: 1972 2205 2392 2536 2903 3288 3305 4757 4843 Size: 233 187 144 367 385 17 1452 86 Cuts at: 4843 4855 5201 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 233 187 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2093 2093 Size: 5206 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 513 Size: 552 939 147 3568 Fragments arranged by size: 3568 939 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4467 Fragments arranged by size: 4467 683 56 BtsI GCAGTG_nn' Cuts at: 2722 3516 3536 2722 Size: 794 20 4392 Fragments arranged by size: 4392 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5206 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5171 DraI TTT'AAA Cuts at: 2510 2510 Size: 5206 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4213 809 Size: 507 2897 1802 Fragments arranged by size: 2897 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2653 3542 5171 Size: 54 1425 50 275 18 823 889 1629 Cuts at: 5171 8 Size: 43 Fragments arranged by size: 1629 1425 889 823 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2653 1537 Size: 275 841 4090 Fragments arranged by size: 4090 841 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3136 1353 Size: 1783 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 3793 4167 4313 387 Size: 1064 138 476 1728 374 146 1280 Fragments arranged by size: 1728 1280 1064 476 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 3269 3866 4653 4921 1239 Size: 393 593 1044 597 787 268 1524 Fragments arranged by size: 1524 1044 787 597 593 393 268 EcoRI G'AATT_C Cuts at: 2515 2893 2515 Size: 378 4828 FspI TGC'GCA Cuts at: 3712 3712 Size: 5206 HaeII r_GCGC'y Cuts at: 1678 4353 1678 Size: 2675 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 3847 3879 3921 Size: 32 135 32 618 32 1625 32 42 Cuts at: 3921 3953 1373 Size: 32 2626 Fragments arranged by size: 2626 1625 618 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2221 2550 2796 669 Size: 988 281 223 60 329 246 3079 Fragments arranged by size: 3079 988 329 281 246 223 60 HindIII A'AGCT_T Cuts at: 748 748 Size: 5206 HpaI GTT'AAC Cuts at: 2796 2796 Size: 5206 KpnI G_GTAC'C Cuts at: 2531 2531 Size: 5206 MfeI C'AATT_G Cuts at: 2805 2805 Size: 5206 MluI A'CGCG_T Cuts at: 5082 5082 Size: 5206 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2436 4320 4504 1305 Size: 1131 1884 184 2007 Fragments arranged by size: 2007 1884 1131 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3384 Fragments arranged by size: 3384 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2105 3164 3523 3682 518 Size: 648 939 1059 359 159 2042 Fragments arranged by size: 2042 1059 939 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2622 3299 4447 4692 1606 Size: 1016 677 1148 245 2120 Fragments arranged by size: 2120 1148 1016 677 245 NaeI GCC'GGC Cuts at: 2080 2080 Size: 5206 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3715 Fragments arranged by size: 3715 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5206 NgoMIV G'CCGG_C Cuts at: 2078 2078 Size: 5206 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5206 NotI GC'GGCC_GC Cuts at: 2653 2653 Size: 5206 NspI r_CATG'y Cuts at: 1132 1229 2049 2071 2104 2564 4109 1132 Size: 97 820 22 33 460 1545 2229 Fragments arranged by size: 2229 1545 820 460 97 33 22 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5206 PciI A'CATG_T Cuts at: 4105 4105 Size: 5206 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5206 PfoI T'CCnGG_A Cuts at: 2419 2419 Size: 5206 PmeI GTTT'AAAC Cuts at: 2510 2510 Size: 5206 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3275 3307 4788 4820 3275 Size: 32 1481 32 3661 Fragments arranged by size: 3661 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5206 PsiI TTA'TAA Cuts at: 2776 2776 Size: 5206 PstI C_TGCA'G Cuts at: 830 2558 830 Size: 1728 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5206 SalI G'TCGA_C Cuts at: 1936 2548 1936 Size: 612 4594 SbfI CC_TGCA'GG Cuts at: 2558 2558 Size: 5206 ScaI AGT'ACT Cuts at: 3454 5166 3454 Size: 1712 3494 SfcI C'TryA_G Cuts at: 826 1045 2554 3689 4370 4561 826 Size: 219 1509 1135 681 191 1471 Fragments arranged by size: 1509 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5206 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5206 SmaI CCC'GGG Cuts at: 1872 2533 1872 Size: 661 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3310 4211 4473 820 Size: 197 926 1367 901 262 1553 Fragments arranged by size: 1553 1367 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2997 493 Size: 2504 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5206 SphI G_CATG'C Cuts at: 1132 2049 2564 1132 Size: 917 515 3774 Fragments arranged by size: 3774 917 515 SspI AAT'ATT Cuts at: 5 52 3130 5 Size: 47 3078 2081 Fragments arranged by size: 3078 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5206 StyI C'CwwG_G Cuts at: 513 1065 2004 2062 2645 513 Size: 552 939 58 583 3074 Fragments arranged by size: 3074 939 583 552 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 3233 3250 3403 3588 4033 325 Size: 1255 639 1014 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1014 639 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3452 5164 96 Size: 274 80 33 51 1443 1475 1712 138 Fragments arranged by size: 1712 1475 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2034 2066 5166 5198 2034 Size: 32 3100 32 2042 Fragments arranged by size: 3100 2042 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2126 1316 Size: 810 4396 XbaI T'CTAG_A Cuts at: 2542 2542 Size: 5206 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5206 XmaI C'CCGG_G Cuts at: 1870 2531 1870 Size: 661 4545 XmnI GAAnn'nnTTC Cuts at: 3335 3335 Size: 5206 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3014 276 Size: 53 83 186 936 1480 2468 Fragments arranged by size: 2468 1480 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI