(Linear) (Six-base) MAPSORT of: KIEE6188.seq  Check: 4583  from: 1  to: 501



KIEE6188.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     501 
   Size:        220     281
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     243     501 
   Size:        132     111     258

  Fragments arranged by size: 

                 258     132     111
 
BamHI G'GATC_C

Cuts at:      0     200     501 
   Size:        200     301
 
BanI G'GyrC_C

Cuts at:      0     362     501 
   Size:        362     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42     501 
   Size:         42     459
 
Bpu10I CC'TnA_GC

Cuts at:      0     284     501 
   Size:        284     217
 
BsaBI GATnn'nnATC

Cuts at:      0     205     501 
   Size:        205     296
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      29      59     501 
   Size:         29      30     442

  Fragments arranged by size: 

                 442      30      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     188     501 
   Size:        188     313
 
BsrDI GCAATG_nn'

Cuts at:      0     332     501 
   Size:        332     169
 
BsrFI r'CCGG_y

Cuts at:      0      73     501 
   Size:         73     428
 
BstYI r'GATC_y

Cuts at:      0     200     387     501 
   Size:        200     187     114

  Fragments arranged by size: 

                 200     187     114
 
Bsu36I CC'TnA_GG

Cuts at:      0      88     501 
   Size:         88     413
 
BtgI C'CryG_G

Cuts at:      0     146     501 
   Size:        146     355
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      60     501 
   Size:         60     441
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     501 
   Size:        220     281
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     185     217     501 
   Size:        185      32     284

  Fragments arranged by size: 

                 284     185      32
 
HincII GTy'rAC

Cuts at:      0     156     216     501 
   Size:        156      60     285

  Fragments arranged by size: 

                 285     156      60
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     501 
   Size:        431      70
 
MslI CAynn'nnrTG

Cuts at:      0     100     501 
   Size:        100     401
 
NaeI GCC'GGC

Cuts at:      0      75     501 
   Size:         75     426
 
NgoMIV G'CCGG_C

Cuts at:      0      73     501 
   Size:         73     428
 
NspI r_CATG'y

Cuts at:      0      44      66      99     501 
   Size:         44      22      33     402

  Fragments arranged by size: 

                 402      44      33      22
 
PfoI T'CCnGG_A

Cuts at:      0     414     501 
   Size:        414      87
 
SphI G_CATG'C

Cuts at:      0      44     501 
   Size:         44     457
 
StyI C'CwwG_G

Cuts at:      0      57     501 
   Size:         57     444
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     214     501 
   Size:        214     287
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29      61     501 
   Size:         29      32     440

  Fragments arranged by size: 

                 440      32      29
 
Tth111I GACn'n_nGTC

Cuts at:      0     121     501 
   Size:        121     380
 

 Enzymes that do cut:

     AcuI    AlwNI    BamHI     BanI     BbsI   Bpu10I    BsaBI    BsaXI
    BsmBI    BsrDI    BsrFI    BstYI   Bsu36I     BtgI     EciI  Eco57MI
    Hin4I   HincII     MmeI     MslI     NaeI   NgoMIV     NspI     PfoI
     SphI     StyI    TaqII     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI