################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmI BspHI BspLU11I BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MunI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SbfI SexAI SfiI SgrAI SmaI SmlI SnaBI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 702 GTCGAC GT^[AC][GT]AC AccIII 1721 TCCGGA T^CCGGA AcsI 988 GAATTT [GA]^AATT[CT] ApoI 988 GAATTT [GA]^AATT[CT] BalI 179 TGGCCA TGG^CCA BamHI 1769 GGATCC G^GATCC BbeI 116 GGCGCC GGCGC^C BbrPI 1132 CACGTG CAC^GTG BbsI 726 GAAGAC GAAGAC(2/6) BbvCI 1303 GCTGAGG CCTCAGC(-5/-2) BcgI 2097 CGACGACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 119 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 726 GAAGAC GAAGAC(2/6) BsaI 187 GGTCTC GGTCTC(1/5) BsaWI 1721 TCCGGA [AT]^CCGG[AT] BseAI 1721 TCCGGA T^CCGGA Bsp1407I 248 TGTACA T^GTACA BspDI 1249 ATCGAT AT^CGAT BspEI 1721 TCCGGA T^CCGGA BsrBI 1906 CCGCTC CCGCTC(-3/-3) BsrFI 1928 GCCGGC [GA]^CCGG[CT] BsrGI 248 TGTACA T^GTACA BstAPI 1131 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1550 TTCGAA TT^CGAA BstYI 1769 GGATCC [GA]^GATC[CT] Cfr10I 1928 GCCGGC [GA]^CCGG[CT] ClaI 1249 ATCGAT AT^CGAT DrdI 1959 GACGCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 179 TGGCCA [CT]^GGCC[GA] EcoNI 1695 CCTGTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1885 GATATC GAT^ATC HincII 702 GTCGAC GT[CT]^[GA]AC HindII 702 GTCGAC GT[CT]^[GA]AC KasI 116 GGCGCC G^GCGCC KspI 1896 CCGCGG CCGC^GG MflI 1769 GGATCC [GA]^GATC[CT] MluNI 179 TGGCCA TGG^CCA MroI 1721 TCCGGA T^CCGGA MscI 179 TGGCCA TGG^CCA MseI 8 TTAA T^TAA NaeI 1928 GCCGGC GCC^GGC NarI 116 GGCGCC GG^CGCC NgoMIV 1928 GCCGGC G^CCGGC NheI 1536 GCTAGC G^CTAGC NspV 1550 TTCGAA TT^CGAA PmaCI 1132 CACGTG CAC^GTG PmlI 1132 CACGTG CAC^GTG PvuII 771 CAGCTG CAG^CTG SacII 1896 CCGCGG CCGC^GG SalI 702 GTCGAC G^TCGAC ScaI 1481 AGTACT AGT^ACT SfoI 116 GGCGCC GGC^GCC SfuI 1550 TTCGAA TT^CGAA SpeI 318 ACTAGT A^CTAGT SphI 1167 GCATGC GCATG^C SspBI 248 TGTACA T^GTACA SspI 1000 AATATT AAT^ATT StuI 1346 AGGCCT AGG^CCT TfiI 1597 GAATC G^A[AT]TC Tru9I 8 TTAA T^TAA XcmI 963 CCAGTCTATTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1769 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 116 GGCGCC G[GA]^CG[CT]C 1959 GACGCC AflIII 1085 ACGTGT A^C[GA][CT]GT 1973 ACGTGT AlwI 1770 GATCC GGATC(4/5) 1769 GGATC AlwNI 161 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 196 CAGCTCCTG AocI 625 CCTGAGG CC^T[ACGT]AGG 1839 CCTGAGG ApaI 1115 GGGCCC GGGCC^C 1565 GGGCCC AvaI 1954 CCCGAG C^[CT]CG[GA]G 1963 CCCGAG BciVI 364 GGATAC GTATCC(6/5) 630 GGATAC BfaI 319 CTAG C^TAG 1537 CTAG BsaHI 116 GGCGCC G[GA]^CG[CT]C 1959 GACGCC BsmBI 610 GAGACG CGTCTC(1/5) 1957 GAGACG BsoBI 1954 CCCGAG C^[CT]CG[GA]G 1963 CCCGAG BspMI 524 ACCTGC ACCTGC(4/8) 842 ACCTGC BsrDI 882 CATTGC GCAATG(2/0) 1361 GCAATG Bsu36I 625 CCTGAGG CC^T[ACGT]AGG 1839 CCTGAGG Eco81I 625 CCTGAGG CC^T[ACGT]AGG 1839 CCTGAGG Hin1I 116 GGCGCC G[GA]^CG[CT]C 1959 GACGCC MaeI 319 CTAG C^TAG 1537 CTAG NcoI 272 CCATGG C^CATGG 635 CCATGG SacI 1375 GAGCTC GAGCT^C 2065 GAGCTC SapI 2062 GAAGAGC GCTCTTC(1/4) 145 GCTCTTC Tsp45I 139 GTCAC ^GT[GC]AC 2087 GTGAC Tsp509I 542 AATT ^AATT 989 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TspRI TthHB8I Van91I