(Circular) (Six-base) MAPSORT of: pFN21AE4196.seq Check: 8200 from: 1 to: 6823 pFN21AE4196 6823 bp With 182 enzymes: SgfI * March 28, 2012 13:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2856 2856 Size: 6823 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2708 4166 1937 Size: 771 1458 4594 Fragments arranged by size: 4594 1458 771 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2750 4886 6270 6538 1632 Size: 1118 2136 1384 268 1917 Fragments arranged by size: 2136 1917 1384 1118 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 3090 3978 5722 6699 1203 Size: 1887 888 1744 977 1327 Fragments arranged by size: 1887 1744 1327 977 888 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 5552 5552 Size: 6823 AleI CACnn'nnGTG Cuts at: 1166 3077 1166 Size: 1911 4912 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2564 3095 3176 1184 Size: 1380 531 81 4831 Fragments arranged by size: 4831 1380 531 81 AlwNI CAG_nnn'CTG Cuts at: 2171 2206 4566 6138 2171 Size: 35 2360 1572 2856 Fragments arranged by size: 2856 2360 1572 35 ApaI G_GGCC'C Cuts at: 3124 3574 3124 Size: 450 6373 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 2993 4132 4358 4510 6619 1493 Size: 1500 1139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1500 1139 226 152 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 3959 3968 4148 1870 Size: 73 2016 9 180 4545 Fragments arranged by size: 4545 2016 180 73 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3220 3253 3220 Size: 33 6790 BamHI G'GATC_C Cuts at: 3774 4153 4520 3774 Size: 379 367 6077 Fragments arranged by size: 6077 379 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2910 3955 4044 4144 Size: 325 217 627 334 789 1045 89 100 Cuts at: 4144 5599 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1045 789 627 334 325 217 100 89 BanII G_rGCy'C Cuts at: 1391 2181 3048 3124 3384 3442 3574 4074 4142 Size: 790 867 76 260 58 132 500 68 Cuts at: 4142 1391 Size: 4072 Fragments arranged by size: 4072 867 790 500 260 132 76 68 58 BbeI G_GCGC'C Cuts at: 2125 2125 Size: 6823 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2738 928 Size: 971 839 5013 Fragments arranged by size: 5013 971 839 BbvCI CC'TCA_GC Cuts at: 3309 3309 Size: 6823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4091 4125 5014 5048 1903 Size: 34 2154 34 889 34 3678 Fragments arranged by size: 3678 2154 889 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2363 2629 4715 5925 2363 Size: 266 2086 1210 3261 Fragments arranged by size: 3261 2086 1210 266 BclI T'GATC_A Cuts at: 1548 1548 Size: 6823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2130 5434 136 Size: 107 122 71 631 605 458 3304 1525 Fragments arranged by size: 3304 1525 631 605 458 122 107 71 BglII A'GATC_T Cuts at: 6818 6818 Size: 6823 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 3124 3574 4049 4884 6040 1790 Size: 1334 450 475 835 1156 2573 Fragments arranged by size: 2573 1334 1156 835 475 450 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2921 3445 4554 Size: 416 53 83 399 24 1504 524 1109 Cuts at: 4554 5512 442 Size: 958 1753 Fragments arranged by size: 1753 1504 1109 958 524 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3545 1056 Size: 2489 4334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2677 3134 3407 3533 3679 5483 1239 Size: 1438 457 273 126 146 1804 2579 Fragments arranged by size: 2579 1804 1438 457 273 146 126 Bpu10I CC'TnA_GC Cuts at: 3309 3309 Size: 6823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4948 5813 6111 4948 Size: 865 298 5660 Fragments arranged by size: 5660 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 5486 882 Size: 617 699 3288 2219 Fragments arranged by size: 3288 2219 699 617 BsaAI yAC'GTr Cuts at: 493 1204 3091 3139 3979 4614 493 Size: 711 1887 48 840 635 2702 Fragments arranged by size: 2702 1887 840 711 635 48 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2122 3965 4630 5012 Size: 53 83 186 936 589 1843 665 382 Cuts at: 5012 5647 275 Size: 635 1451 Fragments arranged by size: 1843 1451 936 665 635 589 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3726 5256 5654 5928 6075 6486 3726 Size: 1530 398 274 147 411 4063 Fragments arranged by size: 4063 1530 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2894 2924 1178 Size: 30 165 30 1491 30 5077 Fragments arranged by size: 5077 1491 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2828 3326 3574 1186 Size: 1642 498 248 4435 Fragments arranged by size: 4435 1642 498 248 BseYI C'CCAG_C Cuts at: 2158 2171 2283 2338 2443 2686 3032 3040 3057 Size: 13 112 55 105 243 346 8 17 Cuts at: 3057 3274 3627 4023 6026 6522 2158 Size: 217 353 396 2003 496 2459 Fragments arranged by size: 2459 2003 496 396 353 346 243 217 112 105 55 17 13 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2434 3119 3136 3881 4039 1163 Size: 573 354 344 685 17 745 158 3947 Fragments arranged by size: 3947 745 685 573 354 344 158 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4273 5034 6062 1540 Size: 275 186 2272 761 1028 2301 Fragments arranged by size: 2301 2272 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3074 3384 4074 4884 4969 6040 3074 Size: 310 690 810 85 1071 3857 Fragments arranged by size: 3857 1071 810 690 310 85 BsmI GAATG_Cn' Cuts at: 4334 4427 4334 Size: 93 6730 BsmBI CGTCTCn'nnnn_ Cuts at: 2609 3956 2609 Size: 1347 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 3048 3074 3124 3384 3442 3574 Size: 399 391 867 26 50 260 58 132 Cuts at: 3574 4049 4074 4142 4884 4969 6040 1391 Size: 475 25 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 867 742 475 399 391 260 132 85 68 58 50 26 25 BspEI T'CCGG_A Cuts at: 3726 6486 3726 Size: 2760 4063 BspHI T'CATG_A Cuts at: 4712 6442 4712 Size: 1730 5093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2538 2856 4178 844 Size: 1694 318 1322 3489 Fragments arranged by size: 3489 1694 1322 318 BsrBI CCG'CTC Cuts at: 1394 1823 3913 4710 1394 Size: 429 2090 797 3507 Fragments arranged by size: 3507 2090 797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2886 3373 5318 5492 65 Size: 1183 1638 487 1945 174 1396 Fragments arranged by size: 1945 1638 1396 1183 487 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3933 5467 5654 1700 Size: 110 2123 1534 187 2869 Fragments arranged by size: 2869 2123 1534 187 110 BsrGI T'GTAC_A Cuts at: 96 2253 96 Size: 2157 4666 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 3142 794 Size: 2348 4475 BstBI TT'CG_AA Cuts at: 3556 5627 3556 Size: 2071 4752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 2338 2392 2899 2338 Size: 54 507 6262 Fragments arranged by size: 6262 507 54 BstYI r'GATC_y Cuts at: 1972 3774 4153 4520 4905 4922 6374 6460 6472 Size: 1802 379 367 385 17 1452 86 12 Cuts at: 6472 6818 1972 Size: 346 1977 Fragments arranged by size: 1977 1802 1452 385 379 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2631 3845 2631 Size: 1214 5609 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2277 2454 2640 3901 513 Size: 552 939 60 213 177 186 1261 3435 Fragments arranged by size: 3435 1261 939 552 213 186 177 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2424 3721 487 Size: 683 56 1198 1297 3589 Fragments arranged by size: 3589 1297 1198 683 56 BtsI GCAGTG_nn' Cuts at: 2145 3392 4107 4339 5133 5153 2145 Size: 1247 715 232 794 20 3815 Fragments arranged by size: 3815 1247 794 715 232 20 ClaI AT'CG_AT Cuts at: 1554 3255 1554 Size: 1701 5122 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6788 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3970 5830 809 Size: 507 2654 1860 1802 Fragments arranged by size: 2654 1860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 4270 5159 Size: 54 1425 50 275 18 354 2086 889 Cuts at: 5159 6788 8 Size: 1629 43 Fragments arranged by size: 2086 1629 1425 889 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4270 1537 Size: 275 2458 4090 Fragments arranged by size: 4090 2458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 3015 4062 4081 4753 1353 Size: 804 858 1047 19 672 3423 Fragments arranged by size: 3423 1047 858 804 672 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2566 3455 3902 4072 5410 5784 Size: 1064 138 977 889 447 170 1338 374 Cuts at: 5784 5930 387 Size: 146 1280 Fragments arranged by size: 1338 1280 1064 977 889 447 374 170 146 138 EcoICRI GAG'CTC Cuts at: 3382 4072 3382 Size: 690 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2677 2750 3134 3407 3533 3679 4886 Size: 393 1045 73 384 273 126 146 1207 Cuts at: 4886 5483 6270 6538 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1207 1045 787 597 393 384 273 268 146 126 73 EcoNI CCTnn'n_nnAGG Cuts at: 3704 3704 Size: 6823 EcoO109I rG'GnC_Cy Cuts at: 2289 2527 2590 2830 3121 3570 3571 3651 2289 Size: 238 63 240 291 449 1 80 5461 Fragments arranged by size: 5461 449 291 240 238 80 63 1 EcoRI G'AATT_C Cuts at: 4132 4510 4132 Size: 378 6445 EcoRV GAT'ATC Cuts at: 3892 3892 Size: 6823 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2125 2172 3940 5970 1678 Size: 447 47 1768 2030 2531 Fragments arranged by size: 2531 2030 1768 447 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 2406 2438 5464 Size: 32 135 32 709 32 93 32 3026 Cuts at: 5464 5496 5538 5570 1373 Size: 32 42 32 2626 Fragments arranged by size: 3026 2626 709 135 93 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 4167 4413 669 Size: 988 281 771 1458 246 3079 Fragments arranged by size: 3079 1458 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KasI G'GCGC_C Cuts at: 2121 2121 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5937 6121 1305 Size: 4632 184 2007 Fragments arranged by size: 4632 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 10 Size: 54 1425 343 354 4647 Fragments arranged by size: 4647 1425 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2025 2390 2927 3077 4781 5140 5299 Size: 648 859 365 537 150 1704 359 159 Cuts at: 5299 518 Size: 2042 Fragments arranged by size: 2042 1704 859 648 537 365 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2307 2778 3883 3903 3924 4042 4239 4916 Size: 701 471 1105 20 21 118 197 677 Cuts at: 4916 6064 6309 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1105 701 677 471 245 197 118 21 20 NaeI GCC'GGC Cuts at: 3935 3935 Size: 6823 NarI GG'CG_CC Cuts at: 2122 2122 Size: 6823 NcoI C'CATG_G Cuts at: 513 1065 2004 2277 2640 513 Size: 552 939 273 363 4696 Fragments arranged by size: 4696 939 552 363 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NgoMIV G'CCGG_C Cuts at: 3933 3933 Size: 6823 NheI G'CTAG_C Cuts at: 1052 3541 1052 Size: 2489 4334 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 3176 3616 4036 4181 5726 1132 Size: 97 1947 440 420 145 1545 2229 Fragments arranged by size: 2229 1947 1545 440 420 145 97 PasI CC'CwG_GG Cuts at: 1348 2662 3124 1348 Size: 1314 462 5047 Fragments arranged by size: 5047 1314 462 PciI A'CATG_T Cuts at: 5722 5722 Size: 6823 PflMI CCAn_nnn'nTGG Cuts at: 1108 2283 3634 3883 1108 Size: 1175 1351 249 4048 Fragments arranged by size: 4048 1351 1175 249 PfoI T'CCnGG_A Cuts at: 3384 3384 Size: 6823 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PmlI CAC'GTG Cuts at: 3139 3139 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 4892 4924 6405 6437 3569 Size: 32 1291 32 1481 32 3955 Fragments arranged by size: 3955 1481 1291 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2289 2527 2590 2830 2289 Size: 238 63 240 6282 Fragments arranged by size: 6282 240 238 63 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PspOMI G'GGCC_C Cuts at: 3120 3570 3120 Size: 450 6373 PstI C_TGCA'G Cuts at: 830 2466 3486 3670 4175 830 Size: 1636 1020 184 505 3478 Fragments arranged by size: 3478 1636 1020 505 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 PvuII CAG'CTG Cuts at: 2778 2778 Size: 6823 SacI G_AGCT'C Cuts at: 3384 4074 3384 Size: 690 6133 SacII CC_GC'GG Cuts at: 3904 3904 Size: 6823 SalI G'TCGA_C Cuts at: 1936 2707 4165 1936 Size: 771 1458 4594 Fragments arranged by size: 4594 1458 771 SanDI GG'GwC_CC Cuts at: 2830 2830 Size: 6823 SapI GCTCTTCn'nnn_ Cuts at: 2157 4062 2157 Size: 1905 4918 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 3488 5071 6783 3488 Size: 1583 1712 3528 Fragments arranged by size: 3528 1712 1583 SfcI C'TryA_G Cuts at: 826 1045 2104 2462 3482 3666 4171 5306 5987 Size: 219 1059 358 1020 184 505 1135 681 Cuts at: 5987 6178 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1059 1020 681 505 358 219 191 184 SfoI GGC'GCC Cuts at: 2123 2123 Size: 6823 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4150 1872 Size: 2278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4927 5828 6090 820 Size: 197 926 2984 901 262 1553 Fragments arranged by size: 2984 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 2323 152 Size: 2171 4652 SphI G_CATG'C Cuts at: 1132 3176 4181 1132 Size: 2044 1005 3774 Fragments arranged by size: 3774 2044 1005 SspI AAT'ATT Cuts at: 5 52 3007 4747 5 Size: 47 2955 1740 2081 Fragments arranged by size: 2955 2081 1740 47 StuI AGG'CCT Cuts at: 1517 3353 1517 Size: 1836 4987 StyI C'CwwG_G Cuts at: 513 1065 2004 2277 2376 2640 2892 4262 513 Size: 552 939 273 99 264 252 1370 3074 Fragments arranged by size: 3074 1370 939 552 273 264 252 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2858 4850 4867 5020 5205 5650 325 Size: 1255 1278 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1278 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 3486 4008 Size: 274 80 33 51 1443 276 1233 522 Cuts at: 4008 5069 6781 96 Size: 1061 1712 138 Fragments arranged by size: 1712 1443 1233 1061 522 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2894 2926 6783 6815 2894 Size: 32 3857 32 2902 Fragments arranged by size: 3857 2902 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2975 2975 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4148 1870 Size: 2278 4545 XmnI GAAnn'nnTTC Cuts at: 4952 4952 Size: 6823 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I DraIII FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI