(Linear) (Six-base) MAPSORT of: KIEE4196.seq Check: 6408 from: 1 to: 2118 KIEE4196.orf With 182 enzymes: SgfI * March 22, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 851 2118 Size: 851 1267 AccI GT'mk_AC Cuts at: 0 703 2118 Size: 703 1415 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 745 2118 Size: 745 1373 AflIII A'CryG_T Cuts at: 0 1085 1973 2118 Size: 1085 888 145 Fragments arranged by size: 1085 888 145 AleI CACnn'nnGTG Cuts at: 0 1072 2118 Size: 1072 1046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 559 1090 1171 2118 Size: 559 531 81 947 Fragments arranged by size: 947 559 531 81 AlwNI CAG_nnn'CTG Cuts at: 0 166 201 2118 Size: 166 35 1917 Fragments arranged by size: 1917 166 35 ApaI G_GGCC'C Cuts at: 0 1119 1569 2118 Size: 1119 450 549 Fragments arranged by size: 1119 549 450 ApoI r'AATT_y Cuts at: 0 988 2118 Size: 988 1130 AvaI C'yCGr_G Cuts at: 0 1954 1963 2118 Size: 1954 9 155 Fragments arranged by size: 1954 155 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1215 1248 2118 Size: 1215 33 870 Fragments arranged by size: 1215 870 33 BamHI G'GATC_C Cuts at: 0 1769 2118 Size: 1769 349 BanI G'GyrC_C Cuts at: 0 116 905 1950 2039 2118 Size: 116 789 1045 89 79 Fragments arranged by size: 1045 789 116 89 79 BanII G_rGCy'C Cuts at: 0 176 1043 1119 1379 1437 1569 2069 2118 Size: 176 867 76 260 58 132 500 49 Fragments arranged by size: 867 500 260 176 132 76 58 49 BbeI G_GCGC'C Cuts at: 0 120 2118 Size: 120 1998 BbsI GAAGACnn'nnnn_ Cuts at: 0 733 2118 Size: 733 1385 BbvCI CC'TCA_GC Cuts at: 0 1304 2118 Size: 1304 814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2086 2118 Size: 2086 32 BciVI GTATCCnnnnn_n' Cuts at: 0 358 624 2118 Size: 358 266 1494 Fragments arranged by size: 1494 358 266 BglI GCCn_nnn'nGGC Cuts at: 0 125 2118 Size: 125 1993 Bme1580I G_kGCm'C Cuts at: 0 1119 1569 2044 2118 Size: 1119 450 475 74 Fragments arranged by size: 1119 475 450 74 BmrI ACTGGGnnnn_n' Cuts at: 0 916 1440 2118 Size: 916 524 678 Fragments arranged by size: 916 678 524 BmtI G_CTAG'C Cuts at: 0 1540 2118 Size: 1540 578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 672 1129 1402 1528 1674 2118 Size: 672 457 273 126 146 444 Fragments arranged by size: 672 457 444 273 146 126 Bpu10I CC'TnA_GC Cuts at: 0 1304 2118 Size: 1304 814 BsaI GGTCTCn'nnnn_ Cuts at: 0 193 2118 Size: 193 1925 BsaAI yAC'GTr Cuts at: 0 1086 1134 1974 2118 Size: 1086 48 840 144 Fragments arranged by size: 1086 840 144 48 BsaHI Gr'CG_yC Cuts at: 0 117 1960 2118 Size: 117 1843 158 Fragments arranged by size: 1843 158 117 BsaWI w'CCGG_w Cuts at: 0 1721 2118 Size: 1721 397 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 889 919 2118 Size: 889 30 1199 Fragments arranged by size: 1199 889 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 823 1321 1569 2118 Size: 823 498 248 549 Fragments arranged by size: 823 549 498 248 BseYI C'CCAG_C Cuts at: 0 153 166 278 333 438 681 1027 1035 Size: 153 13 112 55 105 243 346 8 Cuts at: 1035 1052 1269 1622 2018 2118 Size: 17 217 353 396 100 Fragments arranged by size: 396 353 346 243 217 153 112 105 100 55 17 13 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 429 1114 1131 1876 2034 2118 Size: 85 344 685 17 745 158 84 Fragments arranged by size: 745 685 344 158 85 84 17 BsiHKAI G_wGCw'C Cuts at: 0 1069 1379 2069 2118 Size: 1069 310 690 49 Fragments arranged by size: 1069 690 310 49 BsmBI CGTCTCn'nnnn_ Cuts at: 0 604 1951 2118 Size: 604 1347 167 Fragments arranged by size: 1347 604 167 Bsp1286I G_dGCh'C Cuts at: 0 176 1043 1069 1119 1379 1437 1569 2044 Size: 176 867 26 50 260 58 132 475 Cuts at: 2044 2069 2118 Size: 25 49 Fragments arranged by size: 867 475 260 176 132 58 50 49 26 25 BspEI T'CCGG_A Cuts at: 0 1721 2118 Size: 1721 397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 533 851 2118 Size: 533 318 1267 Fragments arranged by size: 1267 533 318 BsrBI CCG'CTC Cuts at: 0 1908 2118 Size: 1908 210 BsrDI GCAATG_nn' Cuts at: 0 881 1368 2118 Size: 881 487 750 Fragments arranged by size: 881 750 487 BsrFI r'CCGG_y Cuts at: 0 1928 2118 Size: 1928 190 BsrGI T'GTAC_A Cuts at: 0 248 2118 Size: 248 1870 BstAPI GCAn_nnn'nTGC Cuts at: 0 1137 2118 Size: 1137 981 BstBI TT'CG_AA Cuts at: 0 1551 2118 Size: 1551 567 BstXI CCAn_nnnn'nTGG Cuts at: 0 333 387 894 2118 Size: 333 54 507 1224 Fragments arranged by size: 1224 507 333 54 BstYI r'GATC_y Cuts at: 0 1769 2118 Size: 1769 349 Bsu36I CC'TnA_GG Cuts at: 0 626 1840 2118 Size: 626 1214 278 Fragments arranged by size: 1214 626 278 BtgI C'CryG_G Cuts at: 0 59 272 449 635 1896 2118 Size: 59 213 177 186 1261 222 Fragments arranged by size: 1261 222 213 186 177 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 419 1716 2118 Size: 419 1297 402 Fragments arranged by size: 1297 419 402 BtsI GCAGTG_nn' Cuts at: 0 140 1387 2102 2118 Size: 140 1247 715 16 Fragments arranged by size: 1247 715 140 16 ClaI AT'CG_AT Cuts at: 0 1250 2118 Size: 1250 868 DrdI GACnn_nn'nnGTC Cuts at: 0 1965 2118 Size: 1965 153 EaeI y'GGCC_r Cuts at: 0 179 2118 Size: 179 1939 EarI CTCTTCn'nnn_ Cuts at: 0 152 1010 2057 2076 2118 Size: 152 858 1047 19 42 Fragments arranged by size: 1047 858 152 42 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 561 1450 1897 2067 2118 Size: 561 889 447 170 51 Fragments arranged by size: 889 561 447 170 51 EcoICRI GAG'CTC Cuts at: 0 1377 2067 2118 Size: 1377 690 51 Fragments arranged by size: 1377 690 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 672 745 1129 1402 1528 1674 2118 Size: 672 73 384 273 126 146 444 Fragments arranged by size: 672 444 384 273 146 126 73 EcoNI CCTnn'n_nnAGG Cuts at: 0 1699 2118 Size: 1699 419 EcoO109I rG'GnC_Cy Cuts at: 0 284 522 585 825 1116 1565 1566 1646 Size: 284 238 63 240 291 449 1 80 Cuts at: 1646 2118 Size: 472 Fragments arranged by size: 472 449 291 284 240 238 80 63 1 EcoRV GAT'ATC Cuts at: 0 1887 2118 Size: 1887 231 HaeII r_GCGC'y Cuts at: 0 120 167 1935 2118 Size: 120 47 1768 183 Fragments arranged by size: 1768 183 120 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 276 308 401 433 2118 Size: 276 32 93 32 1685 Fragments arranged by size: 1685 276 93 32 32 HincII GTy'rAC Cuts at: 0 704 2118 Size: 704 1414 KasI G'GCGC_C Cuts at: 0 116 2118 Size: 116 2002 MscI TGG'CCA Cuts at: 0 181 2118 Size: 181 1937 MslI CAynn'nnrTG Cuts at: 0 20 385 922 1072 2118 Size: 20 365 537 150 1046 Fragments arranged by size: 1046 537 365 150 20 MspA1I CmG'CkG Cuts at: 0 302 773 1878 1898 1919 2037 2118 Size: 302 471 1105 20 21 118 81 Fragments arranged by size: 1105 471 302 118 81 21 20 NaeI GCC'GGC Cuts at: 0 1930 2118 Size: 1930 188 NarI GG'CG_CC Cuts at: 0 117 2118 Size: 117 2001 NcoI C'CATG_G Cuts at: 0 272 635 2118 Size: 272 363 1483 Fragments arranged by size: 1483 363 272 NgoMIV G'CCGG_C Cuts at: 0 1928 2118 Size: 1928 190 NheI G'CTAG_C Cuts at: 0 1536 2118 Size: 1536 582 NspI r_CATG'y Cuts at: 0 1171 1611 2031 2118 Size: 1171 440 420 87 Fragments arranged by size: 1171 440 420 87 PasI CC'CwG_GG Cuts at: 0 657 1119 2118 Size: 657 462 999 Fragments arranged by size: 999 657 462 PflMI CCAn_nnn'nTGG Cuts at: 0 278 1629 1878 2118 Size: 278 1351 249 240 Fragments arranged by size: 1351 278 249 240 PfoI T'CCnGG_A Cuts at: 0 1379 2118 Size: 1379 739 PmlI CAC'GTG Cuts at: 0 1134 2118 Size: 1134 984 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1564 1596 2118 Size: 1564 32 522 Fragments arranged by size: 1564 522 32 PpuMI rG'GwC_Cy Cuts at: 0 284 522 585 825 2118 Size: 284 238 63 240 1293 Fragments arranged by size: 1293 284 240 238 63 PspOMI G'GGCC_C Cuts at: 0 1115 1565 2118 Size: 1115 450 553 Fragments arranged by size: 1115 553 450 PstI C_TGCA'G Cuts at: 0 461 1481 1665 2118 Size: 461 1020 184 453 Fragments arranged by size: 1020 461 453 184 PvuII CAG'CTG Cuts at: 0 773 2118 Size: 773 1345 SacI G_AGCT'C Cuts at: 0 1379 2069 2118 Size: 1379 690 49 Fragments arranged by size: 1379 690 49 SacII CC_GC'GG Cuts at: 0 1899 2118 Size: 1899 219 SalI G'TCGA_C Cuts at: 0 702 2118 Size: 702 1416 SanDI GG'GwC_CC Cuts at: 0 825 2118 Size: 825 1293 SapI GCTCTTCn'nnn_ Cuts at: 0 152 2057 2118 Size: 152 1905 61 Fragments arranged by size: 1905 152 61 ScaI AGT'ACT Cuts at: 0 1483 2118 Size: 1483 635 SfcI C'TryA_G Cuts at: 0 99 457 1477 1661 2118 Size: 99 358 1020 184 457 Fragments arranged by size: 1020 457 358 184 99 SfoI GGC'GCC Cuts at: 0 118 2118 Size: 118 2000 SpeI A'CTAG_T Cuts at: 0 318 2118 Size: 318 1800 SphI G_CATG'C Cuts at: 0 1171 2118 Size: 1171 947 SspI AAT'ATT Cuts at: 0 1002 2118 Size: 1002 1116 StuI AGG'CCT Cuts at: 0 1348 2118 Size: 1348 770 StyI C'CwwG_G Cuts at: 0 272 371 635 887 2118 Size: 272 99 264 252 1231 Fragments arranged by size: 1231 272 264 252 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 853 2118 Size: 853 1265 TatI w'GTAC_w Cuts at: 0 248 1481 2003 2118 Size: 248 1233 522 115 Fragments arranged by size: 1233 522 248 115 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 889 921 2118 Size: 889 32 1197 Fragments arranged by size: 1197 889 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 970 2118 Size: 970 1148 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SapI ScaI SfcI SfoI SpeI SphI SspI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BclI BfrBI BglII BlpI BmgBI BplI BpuEI BsaBI BsiEI BsiWI BsmI BspHI BssHII BssSI BstEII BstZ17I CspCI DraI DraIII EagI EcoRI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI