################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 556 GAATTT [GA]^AATT[CT] AcyI 16 GGCGCC G[GA]^CG[CT]C AfaI 31 GTAC GT^AC AluI 102 AGCT AG^CT AlwI 228 GATCC GGATC(4/5) AlwNI 523 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 556 GAATTT [GA]^AATT[CT] Asp700I 63 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 493 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 16 GGCGCC G^G[CT][GA]CC BbeI 16 GGCGCC GGCGC^C BcnI 59 CCGGG CC^[GC]GG BsaHI 16 GGCGCC G[GA]^CG[CT]C BsmAI 180 GAGAC GTCTC(1/5) BsmFI 485 GGGAC GGGAC(10/14) BsrBI 564 CCGCTC CCGCTC(-3/-3) BstF5I 342 CATCC GGATG(2/0) BstXI 305 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 383 CGGTCCG CG^G[AT]CCG DpnII 228 GATC ^GATC EcoT14I 510 CCATGG C^C[AT][AT]GG FokI 342 CATCC GGATG(9/13) HgaI 375 GACGC GACGC(5/10) Hin1I 16 GGCGCC G[GA]^CG[CT]C HphI 315 GGTGA GGTGA(8/7) KasI 16 GGCGCC G^GCGCC KspI 22 CCGCGG CCGC^GG MboI 228 GATC ^GATC MboII 279 GAAGA GAAGA(8/7) NarI 16 GGCGCC GG^CGCC NciI 59 CCGGG CC^[GC]GG NcoI 510 CCATGG C^CATGG NdeII 228 GATC ^GATC PflFI 493 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 85 CTGCAG CTGCA^G RsaI 31 GTAC GT^AC RsrII 383 CGGTCCG CG^G[AT]CCG SacII 22 CCGCGG CCGC^GG Sau3AI 228 GATC ^GATC SfcI 85 CTGCAG C^T[GA][CT]AG SfiI 360 GGCCTATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 16 GGCGCC GGC^GCC SmlI 139 CTCAAG C^T[CT][GA]AG StyI 510 CCATGG C^C[AT][AT]GG TaqI 503 TCGA T^CGA Tsp509I 557 AATT ^AATT TspRI 346 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 493 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 503 TCGA T^CGA XmnI 63 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 23 CGCG CG^CG 388 CGCG AvaII 133 GGTCC G^G[AT]CC 384 GGTCC BbvI 417 GCAGC GCAGC(8/12) 84 GCTGC BglI 303 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 361 GCCTATGGGGC BpmI 567 CTCCAG CTGGAG(16/14) 529 CTGGAG BseRI 241 GAGGAG GAGGAG(10/8) 532 GAGGAG BsiYI 7 CCGCTGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 21 CCCGCGGCTGG BslI 7 CCGCTGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 21 CCCGCGGCTGG BsrI 494 ACTGG ACTGG(1/-1) 345 CCAGT BstUI 23 CGCG CG^CG 388 CGCG DdeI 155 CTCAG C^T[ACGT]AG 455 CTGAG DsaI 22 CCGCGG C^C[GA][CT]GG 510 CCATGG HapII 59 CCGG C^CGG 123 CCGG HinfI 182 GACTC G^A[ACGT]TC 457 GAGTC HpaII 59 CCGG C^CGG 123 CCGG MspI 59 CCGG C^CGG 123 CCGG MvnI 23 CGCG CG^CG 388 CGCG PleI 182 GACTC GAGTC(4/5) 457 GAGTC SfaNI 451 GATGC GCATC(5/9) 293 GCATC StuI 209 AGGCCT AGG^CCT 359 AGGCCT TseI 84 GCTGC G^C[AT]GC 417 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI BsaJI BssKI BstNI CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HhaI HinP1I ItaI MaeIII MnlI MspA1I MvaI MwoI NlaIII NlaIV Sau96I ScrFI Tsp45I