(Circular) (Six-base) MAPSORT of: pFN21AE9283.seq Check: 4603 from: 1 to: 5281 pFN21AE9283 5281 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3091 278 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 1937 Size: 687 4594 Acc65I G'GTAC_C Cuts at: 2602 2602 Size: 5281 AclI AA'CG_TT Cuts at: 1563 3408 3781 1563 Size: 1845 373 3063 Fragments arranged by size: 3063 1845 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3344 4728 4996 1632 Size: 1712 1384 268 1917 Fragments arranged by size: 1917 1712 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5084 AflIII A'CryG_T Cuts at: 1203 4180 5157 1203 Size: 2977 977 1327 Fragments arranged by size: 2977 1327 977 AgeI A'CCGG_T Cuts at: 4112 4112 Size: 5281 AhdI GACnn_n'nnGTC Cuts at: 4010 4010 Size: 5281 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5281 AlwNI CAG_nnn'CTG Cuts at: 2533 3024 4596 2533 Size: 491 1572 3218 Fragments arranged by size: 3218 1572 491 ApaLI G'TGCA_C Cuts at: 3338 4494 3338 Size: 1156 4125 ApoI r'AATT_y Cuts at: 1493 2561 2590 2816 2968 5077 1493 Size: 1068 29 226 152 2109 1697 Fragments arranged by size: 2109 1697 1068 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 3835 160 Size: 872 2803 1606 Fragments arranged by size: 2803 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 AvaI C'yCGr_G Cuts at: 1870 1943 2606 1870 Size: 73 663 4545 Fragments arranged by size: 4545 663 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2027 2060 2027 Size: 33 5248 BamHI G'GATC_C Cuts at: 2611 2978 2611 Size: 367 4914 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2602 4057 618 Size: 325 217 627 234 581 1455 1842 Fragments arranged by size: 1842 1455 627 581 325 234 217 BanII G_rGCy'C Cuts at: 1391 2600 1391 Size: 1209 4072 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 5281 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3472 3506 1903 Size: 34 1535 34 3678 Fragments arranged by size: 3678 1535 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3173 4383 3173 Size: 1210 4071 BclI T'GATC_A Cuts at: 1548 1548 Size: 5281 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2314 2372 3892 Size: 107 122 71 631 605 642 58 1520 Cuts at: 3892 136 Size: 1525 Fragments arranged by size: 1525 1520 642 631 605 122 107 71 58 BglII A'GATC_T Cuts at: 5276 5276 Size: 5281 BlpI GC'TnA_GC Cuts at: 2698 2698 Size: 5281 Bme1580I G_kGCm'C Cuts at: 1790 3342 4498 1790 Size: 1552 1156 2573 Fragments arranged by size: 2573 1552 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2509 3012 3970 Size: 416 53 83 399 24 1092 503 958 Cuts at: 3970 442 Size: 1753 Fragments arranged by size: 1753 1092 958 503 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5281 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 5281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 2557 3941 1239 Size: 1316 2 1384 2579 Fragments arranged by size: 2579 1384 1316 2 Bpu10I CC'TnA_GC Cuts at: 2160 2160 Size: 5281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 3406 4271 4569 2129 Size: 1277 865 298 2841 Fragments arranged by size: 2841 1277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3944 882 Size: 617 2445 2219 Fragments arranged by size: 2445 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3072 493 Size: 711 1868 2702 Fragments arranged by size: 2702 1868 711 BsaBI GATnn'nnATC Cuts at: 5275 5275 Size: 5281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3088 3470 4105 Size: 53 83 186 936 489 1066 382 635 Cuts at: 4105 275 Size: 1451 Fragments arranged by size: 1451 1066 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3714 4112 4386 4533 4944 3714 Size: 398 274 147 411 4051 Fragments arranged by size: 4051 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2097 2127 2528 2558 1178 Size: 30 165 30 694 30 401 30 3901 Fragments arranged by size: 3901 694 401 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2552 1186 Size: 1075 291 3915 Fragments arranged by size: 3915 1075 291 BseYI C'CCAG_C Cuts at: 2014 2238 2304 2430 4484 4980 2014 Size: 224 66 126 2054 496 2315 Fragments arranged by size: 2315 2054 496 224 126 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4708 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 3492 4520 1540 Size: 275 186 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 186 BsiHKAI G_wGCw'C Cuts at: 3342 3427 4498 3342 Size: 85 1071 4125 Fragments arranged by size: 4125 1071 85 BsmI GAATG_Cn' Cuts at: 2792 2885 2792 Size: 93 5188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2600 3342 3427 4498 1391 Size: 399 810 742 85 1071 2174 Fragments arranged by size: 2174 1071 810 742 399 85 BspEI T'CCGG_A Cuts at: 4944 4944 Size: 5281 BspHI T'CATG_A Cuts at: 3170 4900 3170 Size: 1730 3551 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2636 844 Size: 1792 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2571 3168 1394 Size: 429 748 597 3507 Fragments arranged by size: 3507 748 597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3776 3950 65 Size: 1183 2528 174 1396 Fragments arranged by size: 2528 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3925 4112 1700 Size: 110 2115 187 2869 Fragments arranged by size: 2869 2115 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5281 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5281 BssSI C'ACGA_G Cuts at: 3341 4353 3341 Size: 1012 4269 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5281 BstBI TT'CG_AA Cuts at: 4085 4085 Size: 5281 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5281 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2317 Size: 5281 BstYI r'GATC_y Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276 Size: 639 367 385 17 1452 86 12 346 Cuts at: 5276 1972 Size: 1977 Fragments arranged by size: 1977 1452 639 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 2515 513 Size: 552 939 23 488 3279 Fragments arranged by size: 3279 939 552 488 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4542 Fragments arranged by size: 4542 683 56 BtsI GCAGTG_nn' Cuts at: 2797 3591 3611 2797 Size: 794 20 4467 Fragments arranged by size: 4467 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5281 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5246 DraI TTT'AAA Cuts at: 2585 2585 Size: 5281 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4288 809 Size: 507 2972 1802 Fragments arranged by size: 2972 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 3617 5246 Size: 54 1425 50 275 18 898 889 1629 Cuts at: 5246 8 Size: 43 Fragments arranged by size: 1629 1425 898 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2728 1537 Size: 275 916 4090 Fragments arranged by size: 4090 916 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3211 1353 Size: 1858 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3868 4242 4388 387 Size: 1064 138 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2555 2557 3344 3941 4728 4996 1239 Size: 393 923 2 787 597 787 268 1524 Fragments arranged by size: 1524 923 787 787 597 393 268 2 EcoRI G'AATT_C Cuts at: 2590 2968 2590 Size: 378 4903 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 5281 FspI TGC'GCA Cuts at: 3787 3787 Size: 5281 HaeII r_GCGC'y Cuts at: 1678 2025 2090 2533 4428 1678 Size: 347 65 443 1895 2531 Fragments arranged by size: 2531 1895 443 347 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2447 2479 3922 Size: 32 135 32 525 32 318 32 1443 Cuts at: 3922 3954 3996 4028 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 525 318 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2625 2871 669 Size: 988 281 687 246 3079 Fragments arranged by size: 3079 988 687 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5281 HpaI GTT'AAC Cuts at: 2871 2871 Size: 5281 KasI G'GCGC_C Cuts at: 2021 2021 Size: 5281 KpnI G_GTAC'C Cuts at: 2606 2606 Size: 5281 MfeI C'AATT_G Cuts at: 2880 2880 Size: 5281 MluI A'CGCG_T Cuts at: 5157 5157 Size: 5281 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 2526 4395 4579 1305 Size: 1080 141 1869 184 2007 Fragments arranged by size: 2007 1869 1080 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3459 Fragments arranged by size: 3459 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3239 3598 3757 518 Size: 648 2073 359 159 2042 Fragments arranged by size: 2073 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2029 2049 2238 2425 2697 3374 4522 Size: 408 15 20 189 187 272 677 1148 Cuts at: 4522 4767 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 408 272 245 189 187 20 15 NarI GG'CG_CC Cuts at: 2022 2022 Size: 5281 NcoI C'CATG_G Cuts at: 513 1065 2004 2515 513 Size: 552 939 511 3279 Fragments arranged by size: 3279 939 552 511 NdeI CA'TA_TG Cuts at: 387 387 Size: 5281 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5281 NotI GC'GGCC_GC Cuts at: 2728 2728 Size: 5281 NspI r_CATG'y Cuts at: 1132 1229 2639 4184 1132 Size: 97 1410 1545 2229 Fragments arranged by size: 2229 1545 1410 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5281 PciI A'CATG_T Cuts at: 4180 4180 Size: 5281 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5281 PfoI T'CCnGG_A Cuts at: 2063 2190 2063 Size: 127 5154 PmeI GTTT'AAAC Cuts at: 2585 2585 Size: 5281 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 4863 4895 3350 Size: 32 1481 32 3736 Fragments arranged by size: 3736 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5281 PsiI TTA'TAA Cuts at: 2851 2851 Size: 5281 PstI C_TGCA'G Cuts at: 830 2094 2633 830 Size: 1264 539 3478 Fragments arranged by size: 3478 1264 539 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5281 RsrII CG'GwC_CG Cuts at: 2389 2389 Size: 5281 SacII CC_GC'GG Cuts at: 2030 2030 Size: 5281 SalI G'TCGA_C Cuts at: 1936 2623 1936 Size: 687 4594 SbfI CC_TGCA'GG Cuts at: 2633 2633 Size: 5281 ScaI AGT'ACT Cuts at: 3529 5241 3529 Size: 1712 3569 SfcI C'TryA_G Cuts at: 826 1045 2090 2629 3764 4445 4636 826 Size: 219 1045 539 1135 681 191 1471 Fragments arranged by size: 1471 1135 1045 681 539 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2372 2372 Size: 5281 SfoI GGC'GCC Cuts at: 2023 2023 Size: 5281 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5281 SmaI CCC'GGG Cuts at: 1872 2608 1872 Size: 736 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 3385 4286 4548 820 Size: 197 926 201 1241 901 262 1553 Fragments arranged by size: 1553 1241 926 901 262 201 197 SnaBI TAC'GTA Cuts at: 493 3072 493 Size: 2579 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5281 SphI G_CATG'C Cuts at: 1132 2639 1132 Size: 1507 3774 SspI AAT'ATT Cuts at: 5 52 3205 5 Size: 47 3153 2081 Fragments arranged by size: 3153 2081 47 StuI AGG'CCT Cuts at: 1517 2216 2366 1517 Size: 699 150 4432 Fragments arranged by size: 4432 699 150 StyI C'CwwG_G Cuts at: 513 1065 2004 2515 2720 513 Size: 552 939 511 205 3074 Fragments arranged by size: 3074 939 552 511 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 2422 3308 3325 3478 3663 4108 Size: 1255 516 326 886 17 153 185 445 Cuts at: 4108 325 Size: 1498 Fragments arranged by size: 1498 1255 886 516 445 326 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3527 5239 96 Size: 274 80 33 51 1443 1550 1712 138 Fragments arranged by size: 1712 1550 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5241 5273 5241 Size: 32 5249 Tth111I GACn'n_nGTC Cuts at: 1316 2501 1316 Size: 1185 4096 XbaI T'CTAG_A Cuts at: 2617 2617 Size: 5281 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5281 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5281 XmaI C'CCGG_G Cuts at: 1870 2606 1870 Size: 736 4545 XmnI GAAnn'nnTTC Cuts at: 2072 3410 2072 Size: 1338 3943 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3089 276 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SapI SexAI SrfI SwaI