################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspI StuI StyI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 533 GGTACC G^GTACC AccII 135 CGCG CG^CG AflIII 100 ACACGT A^C[GA][CT]GT AlwNI 1144 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 398 GGGCCC GGGCC^C Asp718I 533 GGTACC G^GTACC BanII 398 GGGCCC G[GA]GC[CT]^C BbrPI 101 CACGTG CAC^GTG BclI 83 TGATCA T^GATCA BfaI 557 CTAG C^TAG BglI 703 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 101 CACGTG [CT]AC^GT[GA] BsaI 6 GAGACC GGTCTC(1/5) BsaWI 1025 ACCGGA [AT]^CCGG[AT] BsmI 792 GAATGC GAATGC(1/-1) Bsp1407I 176 TGTACA T^GTACA BsrDI 501 CATTGC GCAATG(2/0) BsrGI 176 TGTACA T^GTACA BssSI 475 CTCGTG CACGAG(-5/-1) BstUI 135 CGCG CG^CG BstXI 959 CCAACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 704 CCGTGG C^C[GA][CT]GG EaeI 701 TGGCCG [CT]^GGCC[GA] FbaI 83 TGATCA T^GATCA KpnI 533 GGTACC GGTAC^C MaeI 557 CTAG C^TAG MspA1I 1105 CCGCTG C[AC]G^C[GT]G MvnI 135 CGCG CG^CG PmaCI 101 CACGTG CAC^GTG PmlI 101 CACGTG CAC^GTG PstI 797 CTGCAG CTGCA^G SfcI 797 CTGCAG C^T[GA][CT]AG SfiI 702 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 556 ACTAGT A^CTAGT SspBI 176 TGTACA T^GTACA Tsp45I 950 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 497 GGATC GGATC(4/5) 785 GGATC AvaII 512 GGACC G^G[AT]CC 972 GGACC BbsI 438 GAAGAC GAAGAC(2/6) 1033 GAAGAC BpuAI 438 GAAGAC GAAGAC(2/6) 1033 GAAGAC BseRI 658 CTCCTC GAGGAG(10/8) 918 GAGGAG BsmAI 6 GAGAC GTCTC(1/5) 656 GTCTC BsmFI 617 GGGAC GGGAC(10/14) 384 GTCCC DdeI 770 CTCAG C^T[ACGT]AG 946 CTGAG DraII 397 GGGGCCC [GA]G^G[ACGT]CC[CT] 398 GGGCCCT EarI 115 CTCTTC CTCTTC(1/4) 367 CTCTTC EcoO109I 397 GGGGCCC [GA]G^G[ACGT]CC[CT] 398 GGGCCCT FauI 386 CCCGC CCCGC(4/6) 425 CCCGC HgaI 441 GACGC GACGC(5/10) 923 GCGTC Ksp632I 115 CTCTTC CTCTTC(1/4) 367 CTCTTC MseI 826 TTAA T^TAA 1006 TTAA MslI 97 CACACACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 963 CACTCTGGTG SapI 114 GCTCTTC GCTCTTC(1/4) 366 GCTCTTC SmaI 275 CCCGGG CCC^GGG 1169 CCCGGG Tru9I 826 TTAA T^TAA 1006 TTAA XmaCI 275 CCCGGG C^CCGGG 1169 CCCGGG XmaI 275 CCCGGG C^CCGGG 1169 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AvaI BanI BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsoBI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I XcmI