(Circular) (Six-base) MAPSORT of: pFN21AE4413.seq Check: 3700 from: 1 to: 5983 pFN21AE4413 5983 bp With 182 enzymes: SgfI * March 28, 2012 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 2538 3304 2538 Size: 766 5217 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4046 5430 5698 1632 Size: 2414 1384 268 1917 Fragments arranged by size: 2414 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 2105 4882 5859 1203 Size: 902 2777 977 1327 Fragments arranged by size: 2777 1327 977 902 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 2106 2972 1166 Size: 940 866 4177 Fragments arranged by size: 4177 940 866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2492 1184 Size: 1308 4675 AlwNI CAG_nnn'CTG Cuts at: 3154 3726 5298 3154 Size: 572 1572 3839 Fragments arranged by size: 3839 1572 572 ApaI G_GGCC'C Cuts at: 2407 2407 Size: 5983 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2280 2346 3174 3308 1870 Size: 73 337 66 828 134 4545 Fragments arranged by size: 4545 828 337 134 73 66 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2538 2576 2629 3197 3304 Size: 325 217 627 751 38 53 568 107 Cuts at: 3304 4759 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 751 627 568 325 217 107 53 38 BanII G_rGCy'C Cuts at: 1391 2407 3302 1391 Size: 1016 895 4072 Fragments arranged by size: 4072 1016 895 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2450 3045 928 Size: 971 551 595 3866 Fragments arranged by size: 3866 971 595 551 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 2088 1548 Size: 540 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2714 4594 136 Size: 107 122 71 631 605 1042 1880 1525 Fragments arranged by size: 1880 1525 1042 631 605 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2407 2634 3200 4044 5200 1790 Size: 617 227 566 844 1156 2573 Fragments arranged by size: 2573 1156 844 617 566 227 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 3065 3086 3098 4643 1239 Size: 974 852 21 12 1545 2579 Fragments arranged by size: 2579 1545 974 852 21 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4973 5271 4108 Size: 865 298 4820 Fragments arranged by size: 4820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2005 4646 882 Size: 617 506 2641 2219 Fragments arranged by size: 2641 2219 617 506 BsaAI yAC'GTr Cuts at: 493 1204 2108 3774 493 Size: 711 904 1666 2702 Fragments arranged by size: 2702 1666 904 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3790 4172 4807 275 Size: 53 83 186 936 2257 382 635 1451 Fragments arranged by size: 2257 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3030 4416 4814 5088 5235 5646 3030 Size: 1386 398 274 147 411 3367 Fragments arranged by size: 3367 1386 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 1178 Size: 30 165 30 858 30 4870 Fragments arranged by size: 4870 858 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 2938 1186 Size: 1468 284 4231 Fragments arranged by size: 4231 1468 284 BseYI C'CCAG_C Cuts at: 5186 5682 5186 Size: 496 5487 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2674 2703 1163 Size: 573 609 329 29 4443 Fragments arranged by size: 4443 609 573 329 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4044 4129 5200 4044 Size: 85 1071 4827 Fragments arranged by size: 4827 1071 85 BsmI GAATG_Cn' Cuts at: 2803 3494 3587 2803 Size: 691 93 5199 Fragments arranged by size: 5199 691 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2407 2634 3200 3302 4044 4129 5200 Size: 399 617 227 566 102 742 85 1071 Cuts at: 5200 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 617 566 399 227 102 85 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 2439 2451 2763 3872 5602 2439 Size: 12 312 1109 1730 2820 Fragments arranged by size: 2820 1730 1109 312 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 4478 4652 65 Size: 1183 1257 1973 174 1396 Fragments arranged by size: 1973 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 2181 96 Size: 2085 3898 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 2480 4043 5055 2480 Size: 1563 1012 3408 Fragments arranged by size: 3408 1563 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2971 2971 Size: 5983 BstYI r'GATC_y Cuts at: 1972 3313 3680 4065 4082 5534 5620 5632 5978 Size: 1341 367 385 17 1452 86 12 346 Cuts at: 5978 1972 Size: 1977 Fragments arranged by size: 1977 1452 1341 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2709 513 Size: 552 939 705 3787 Fragments arranged by size: 3787 939 705 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2077 3103 487 Size: 683 56 851 1026 3367 Fragments arranged by size: 3367 1026 851 683 56 BtsI GCAGTG_nn' Cuts at: 2691 3198 3499 4293 4313 2691 Size: 507 301 794 20 4361 Fragments arranged by size: 4361 794 507 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2706 3430 4319 Size: 54 1425 50 275 18 876 724 889 Cuts at: 4319 5948 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 876 724 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2378 3913 1353 Size: 773 252 1535 3423 Fragments arranged by size: 3423 1535 773 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2167 2300 4570 4944 5090 387 Size: 1064 138 578 133 2270 374 146 1280 Fragments arranged by size: 2270 1280 1064 578 374 146 138 133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 3065 3086 3098 4046 4643 5430 Size: 393 581 852 21 12 948 597 787 Cuts at: 5430 5698 1239 Size: 268 1524 Fragments arranged by size: 1524 948 852 787 597 581 393 268 21 12 EcoO109I rG'GnC_Cy Cuts at: 2403 2404 2403 Size: 1 5982 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2437 2437 Size: 5983 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 5130 1678 Size: 3452 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 2758 2790 4624 Size: 32 135 32 800 32 354 32 1834 Cuts at: 4624 4656 4698 4730 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1834 800 354 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 2542 3308 2542 Size: 766 5217 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4161 Fragments arranged by size: 4161 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2106 2972 3941 4300 4459 518 Size: 648 940 866 969 359 159 2042 Fragments arranged by size: 2042 969 940 866 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3112 3399 4076 5224 5469 1606 Size: 1506 287 677 1148 245 2120 Fragments arranged by size: 2120 1506 1148 677 287 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4492 Fragments arranged by size: 4492 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 3341 4886 1132 Size: 97 2112 1545 2229 Fragments arranged by size: 2229 2112 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PmlI CAC'GTG Cuts at: 2108 2108 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2254 4052 4084 5565 5597 2222 Size: 32 1798 32 1481 32 2608 Fragments arranged by size: 2608 1798 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PspOMI G'GGCC_C Cuts at: 2403 2403 Size: 5983 PstI C_TGCA'G Cuts at: 830 2806 3335 830 Size: 1976 529 3478 Fragments arranged by size: 3478 1976 529 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 2126 2378 2126 Size: 252 5731 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2802 3331 4466 5147 5338 826 Size: 219 1757 529 1135 681 191 1471 Fragments arranged by size: 1757 1471 1135 681 529 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2714 2714 Size: 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 2282 3176 3310 1872 Size: 410 894 134 4545 Fragments arranged by size: 4545 894 410 134 SmlI C'TyrA_G Cuts at: 820 1017 1943 4087 4988 5250 820 Size: 197 926 2144 901 262 1553 Fragments arranged by size: 2144 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 2561 152 Size: 2409 3574 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 2004 3422 513 Size: 552 939 1418 3074 Fragments arranged by size: 3074 1418 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2564 2669 3218 4010 4027 4180 4365 Size: 1255 984 105 549 792 17 153 185 Cuts at: 4365 4810 325 Size: 445 1498 Fragments arranged by size: 1498 1255 984 792 549 445 185 153 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2232 4229 Size: 274 80 33 51 1443 204 51 1997 Cuts at: 4229 5941 96 Size: 1712 138 Fragments arranged by size: 1997 1712 1443 274 204 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2097 2129 2509 2541 2938 2970 5943 5975 2097 Size: 32 380 32 397 32 2973 32 2105 Fragments arranged by size: 2973 2105 397 380 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2077 2222 2642 2971 2035 Size: 42 145 420 329 5047 Fragments arranged by size: 5047 420 329 145 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 2280 3174 3308 1870 Size: 410 894 134 4545 Fragments arranged by size: 4545 894 410 134 XmnI GAAnn'nnTTC Cuts at: 4112 4112 Size: 5983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI