Restriction map for
pFN21AE4413
(Linear) (Six-base) MAPSORT of: KIEE4413.seq Check: 9035 from: 1 to: 1278
KIEE4413.orf
With 182 enzymes: SgfI *
March 22, 2012 17:57 ..
Acc65I G'GTAC_C
Cuts at: 0 533 1278
Size: 533 745
AflIII A'CryG_T
Cuts at: 0 100 1278
Size: 100 1178
AleI CACnn'nnGTG
Cuts at: 0 101 967 1278
Size: 101 866 311
Fragments arranged by size:
866 311 101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 1278
Size: 487 791
AlwNI CAG_nnn'CTG
Cuts at: 0 1149 1278
Size: 1149 129
ApaI G_GGCC'C
Cuts at: 0 402 1278
Size: 402 876
AvaI C'yCGr_G
Cuts at: 0 275 341 1169 1278
Size: 275 66 828 109
Fragments arranged by size:
828 275 109 66
BanI G'GyrC_C
Cuts at: 0 533 571 624 1192 1278
Size: 533 38 53 568 86
Fragments arranged by size:
568 533 86 53 38
BanII G_rGCy'C
Cuts at: 0 402 1278
Size: 402 876
BbsI GAAGACnn'nnnn_
Cuts at: 0 445 1040 1278
Size: 445 595 238
Fragments arranged by size:
595 445 238
BclI T'GATC_A
Cuts at: 0 83 1278
Size: 83 1195
BglI GCCn_nnn'nGGC
Cuts at: 0 709 1278
Size: 709 569
Bme1580I G_kGCm'C
Cuts at: 0 402 629 1195 1278
Size: 402 227 566 83
Fragments arranged by size:
566 402 227 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1060 1081 1093 1278
Size: 208 852 21 12 185
Fragments arranged by size:
852 208 185 21 12
BsaAI yAC'GTr
Cuts at: 0 103 1278
Size: 103 1175
BsaWI w'CCGG_w
Cuts at: 0 1025 1278
Size: 1025 253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 1278
Size: 256 30 992
Fragments arranged by size:
992 256 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 933 1278
Size: 649 284 345
Fragments arranged by size:
649 345 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 669 698 1278
Size: 340 329 29 580
Fragments arranged by size:
580 340 329 29
BsmI GAATG_Cn'
Cuts at: 0 798 1278
Size: 798 480
Bsp1286I G_dGCh'C
Cuts at: 0 402 629 1195 1278
Size: 402 227 566 83
Fragments arranged by size:
566 402 227 83
BspHI T'CATG_A
Cuts at: 0 434 446 758 1278
Size: 434 12 312 520
Fragments arranged by size:
520 434 312 12
BsrDI GCAATG_nn'
Cuts at: 0 500 1278
Size: 500 778
BsrGI T'GTAC_A
Cuts at: 0 176 1278
Size: 176 1102
BssSI C'ACGA_G
Cuts at: 0 475 1278
Size: 475 803
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 1278
Size: 966 312
BtgI C'CryG_G
Cuts at: 0 704 1278
Size: 704 574
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 72 1098 1278
Size: 72 1026 180
Fragments arranged by size:
1026 180 72
BtsI GCAGTG_nn'
Cuts at: 0 686 1193 1278
Size: 686 507 85
Fragments arranged by size:
686 507 85
EaeI y'GGCC_r
Cuts at: 0 701 1278
Size: 701 577
EarI CTCTTCn'nnn_
Cuts at: 0 121 373 1278
Size: 121 252 905
Fragments arranged by size:
905 252 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 162 295 1278
Size: 162 133 983
Fragments arranged by size:
983 162 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1060 1081 1093 1278
Size: 208 852 21 12 185
Fragments arranged by size:
852 208 185 21 12
EcoO109I rG'GnC_Cy
Cuts at: 0 398 399 1278
Size: 398 1 879
Fragments arranged by size:
879 398 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 432 1278
Size: 432 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 753 785 1278
Size: 367 32 354 32 493
Fragments arranged by size:
493 367 354 32 32
KpnI G_GTAC'C
Cuts at: 0 537 1278
Size: 537 741
MslI CAynn'nnrTG
Cuts at: 0 101 967 1278
Size: 101 866 311
Fragments arranged by size:
866 311 101
MspA1I CmG'CkG
Cuts at: 0 1107 1278
Size: 1107 171
PmlI CAC'GTG
Cuts at: 0 103 1278
Size: 103 1175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1278
Size: 217 32 1029
Fragments arranged by size:
1029 217 32
PspOMI G'GGCC_C
Cuts at: 0 398 1278
Size: 398 880
PstI C_TGCA'G
Cuts at: 0 801 1278
Size: 801 477
SapI GCTCTTCn'nnn_
Cuts at: 0 121 373 1278
Size: 121 252 905
Fragments arranged by size:
905 252 121
SfcI C'TryA_G
Cuts at: 0 797 1278
Size: 797 481
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 709 1278
Size: 709 569
SmaI CCC'GGG
Cuts at: 0 277 1171 1278
Size: 277 894 107
Fragments arranged by size:
894 277 107
SpeI A'CTAG_T
Cuts at: 0 556 1278
Size: 556 722
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 664 1213 1278
Size: 559 105 549 65
Fragments arranged by size:
559 549 105 65
TatI w'GTAC_w
Cuts at: 0 176 227 1278
Size: 176 51 1051
Fragments arranged by size:
1051 176 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 504 536 933 965 1278
Size: 92 32 380 32 397 32 313
Fragments arranged by size:
397 380 313 92 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 72 217 637 966 1278
Size: 30 42 145 420 329 312
Fragments arranged by size:
420 329 312 145 42 30
XmaI C'CCGG_G
Cuts at: 0 275 1169 1278
Size: 275 894 109
Fragments arranged by size:
894 275 109
Enzymes that do cut:
Acc65I AflIII AleI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BclI BglI Bme1580I BpmI BsaAI BsaWI
BsaXI BseRI BsgI BsmI Bsp1286I BspHI BsrDI BsrGI
BssSI BstXI BtgI BtgZI BtsI EaeI EarI EciI
Eco57MI EcoO109I FalI Hin4I KpnI MslI MspA1I PmlI
PpiI PspOMI PstI SapI SfcI SfiI SmaI SpeI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmnI ZraI