(Linear) (Six-base) MAPSORT of: KIBB3323.seq Check: 2207 from: 1 to: 1506 KIBB3323.orf With 182 enzymes: SgfI * February 5, 2013 10:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 580 1506 Size: 308 272 926 Fragments arranged by size: 926 308 272 AleI CACnn'nnGTG Cuts at: 0 913 943 1506 Size: 913 30 563 Fragments arranged by size: 913 563 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 829 1450 1506 Size: 829 621 56 Fragments arranged by size: 829 621 56 AlwNI CAG_nnn'CTG Cuts at: 0 1080 1506 Size: 1080 426 ApaI G_GGCC'C Cuts at: 0 1237 1506 Size: 1237 269 ApaLI G'TGCA_C Cuts at: 0 961 1506 Size: 961 545 AvaI C'yCGr_G Cuts at: 0 1237 1506 Size: 1237 269 BamHI G'GATC_C Cuts at: 0 111 1506 Size: 111 1395 BanI G'GyrC_C Cuts at: 0 993 1417 1506 Size: 993 424 89 Fragments arranged by size: 993 424 89 BanII G_rGCy'C Cuts at: 0 1125 1237 1506 Size: 1125 112 269 Fragments arranged by size: 1125 269 112 BbeI G_GCGC'C Cuts at: 0 997 1506 Size: 997 509 BbsI GAAGACnn'nnnn_ Cuts at: 0 756 825 1005 1506 Size: 756 69 180 501 Fragments arranged by size: 756 501 180 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 528 562 1149 1183 1506 Size: 528 34 587 34 323 Fragments arranged by size: 587 528 323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 72 1506 Size: 72 1434 BglI GCCn_nnn'nGGC Cuts at: 0 630 1147 1210 1506 Size: 630 517 63 296 Fragments arranged by size: 630 517 296 63 BlpI GC'TnA_GC Cuts at: 0 1214 1506 Size: 1214 292 Bme1580I G_kGCm'C Cuts at: 0 965 1237 1506 Size: 965 272 269 Fragments arranged by size: 965 272 269 BmgBI CAC'GTC Cuts at: 0 879 1506 Size: 879 627 BmtI G_CTAG'C Cuts at: 0 754 1506 Size: 754 752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1039 1139 1506 Size: 1039 100 367 Fragments arranged by size: 1039 367 100 Bpu10I CC'TnA_GC Cuts at: 0 60 1245 1506 Size: 60 1185 261 Fragments arranged by size: 1185 261 60 BsaI GGTCTCn'nnnn_ Cuts at: 0 414 1506 Size: 414 1092 BsaAI yAC'GTr Cuts at: 0 659 663 674 1163 1506 Size: 659 4 11 489 343 Fragments arranged by size: 659 489 343 11 4 BsaHI Gr'CG_yC Cuts at: 0 994 1095 1506 Size: 994 101 411 Fragments arranged by size: 994 411 101 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 100 1506 Size: 37 63 1406 Fragments arranged by size: 1406 63 37 BseYI C'CCAG_C Cuts at: 0 141 194 1495 1506 Size: 141 53 1301 11 Fragments arranged by size: 1301 141 53 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 1143 1205 1207 1506 Size: 489 654 62 2 299 Fragments arranged by size: 654 489 299 62 2 BsiEI CG_ry'CG Cuts at: 0 537 1377 1506 Size: 537 840 129 Fragments arranged by size: 840 537 129 BsiHKAI G_wGCw'C Cuts at: 0 61 740 956 965 1506 Size: 61 679 216 9 541 Fragments arranged by size: 679 541 216 61 9 BsiWI C'GTAC_G Cuts at: 0 659 1506 Size: 659 847 Bsp1286I G_dGCh'C Cuts at: 0 61 740 956 965 1125 1237 1506 Size: 61 679 216 9 160 112 269 Fragments arranged by size: 679 269 216 160 112 61 9 BspHI T'CATG_A Cuts at: 0 227 1506 Size: 227 1279 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 779 1506 Size: 779 727 BsrBI CCG'CTC Cuts at: 0 90 216 702 885 971 1506 Size: 90 126 486 183 86 535 Fragments arranged by size: 535 486 183 126 90 86 BsrDI GCAATG_nn' Cuts at: 0 1062 1506 Size: 1062 444 BsrFI r'CCGG_y Cuts at: 0 629 1506 Size: 629 877 BsrGI T'GTAC_A Cuts at: 0 356 1506 Size: 356 1150 BstXI CCAn_nnnn'nTGG Cuts at: 0 405 1506 Size: 405 1101 BstYI r'GATC_y Cuts at: 0 111 1391 1506 Size: 111 1280 115 Fragments arranged by size: 1280 115 111 BtgI C'CryG_G Cuts at: 0 20 404 911 989 1364 1506 Size: 20 384 507 78 375 142 Fragments arranged by size: 507 384 375 142 78 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1284 1506 Size: 1284 222 DraIII CAC_nnn'GTG Cuts at: 0 598 1506 Size: 598 908 EaeI y'GGCC_r Cuts at: 0 572 1196 1506 Size: 572 624 310 Fragments arranged by size: 624 572 310 EarI CTCTTCn'nnn_ Cuts at: 0 284 556 709 1045 1132 1387 1450 1506 Size: 284 272 153 336 87 255 63 56 Fragments arranged by size: 336 284 272 255 153 87 63 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 127 1506 Size: 127 1379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 580 1039 1139 1506 Size: 308 272 459 100 367 Fragments arranged by size: 459 367 308 272 100 EcoNI CCTnn'n_nnAGG Cuts at: 0 1273 1506 Size: 1273 233 EcoO109I rG'GnC_Cy Cuts at: 0 1202 1234 1506 Size: 1202 32 272 Fragments arranged by size: 1202 272 32 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 720 1029 1506 Size: 720 309 477 Fragments arranged by size: 720 477 309 HaeII r_GCGC'y Cuts at: 0 12 28 622 826 997 1080 1118 1245 Size: 12 16 594 204 171 83 38 127 Cuts at: 1245 1506 Size: 261 Fragments arranged by size: 594 261 204 171 127 83 38 16 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 846 878 1506 Size: 846 32 628 Fragments arranged by size: 846 628 32 HincII GTy'rAC Cuts at: 0 1173 1506 Size: 1173 333 HindIII A'AGCT_T Cuts at: 0 130 1506 Size: 130 1376 KasI G'GCGC_C Cuts at: 0 993 1506 Size: 993 513 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 803 1506 Size: 803 703 MscI TGG'CCA Cuts at: 0 574 1506 Size: 574 932 MslI CAynn'nnrTG Cuts at: 0 205 811 913 943 1426 1506 Size: 205 606 102 30 483 80 Fragments arranged by size: 606 483 205 102 80 30 NaeI GCC'GGC Cuts at: 0 631 1506 Size: 631 875 NarI GG'CG_CC Cuts at: 0 994 1506 Size: 994 512 NcoI C'CATG_G Cuts at: 0 911 989 1364 1506 Size: 911 78 375 142 Fragments arranged by size: 911 375 142 78 NgoMIV G'CCGG_C Cuts at: 0 629 1506 Size: 629 877 NheI G'CTAG_C Cuts at: 0 750 1506 Size: 750 756 NspI r_CATG'y Cuts at: 0 378 464 1314 1431 1506 Size: 378 86 850 117 75 Fragments arranged by size: 850 378 117 86 75 PasI CC'CwG_GG Cuts at: 0 998 1144 1506 Size: 998 146 362 Fragments arranged by size: 998 362 146 PflMI CCAn_nnn'nTGG Cuts at: 0 242 1506 Size: 242 1264 PfoI T'CCnGG_A Cuts at: 0 107 1016 1271 1506 Size: 107 909 255 235 Fragments arranged by size: 909 255 235 107 PmlI CAC'GTG Cuts at: 0 1163 1506 Size: 1163 343 PspOMI G'GGCC_C Cuts at: 0 1233 1506 Size: 1233 273 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 889 921 1506 Size: 889 32 585 Fragments arranged by size: 889 585 32 PstI C_TGCA'G Cuts at: 0 1439 1506 Size: 1439 67 SapI GCTCTTCn'nnn_ Cuts at: 0 709 1045 1506 Size: 709 336 461 Fragments arranged by size: 709 461 336 SfcI C'TryA_G Cuts at: 0 1435 1506 Size: 1435 71 SfoI GGC'GCC Cuts at: 0 995 1506 Size: 995 511 SmaI CCC'GGG Cuts at: 0 1239 1506 Size: 1239 267 SnaBI TAC'GTA Cuts at: 0 659 1506 Size: 659 847 SphI G_CATG'C Cuts at: 0 464 1506 Size: 464 1042 StyI C'CwwG_G Cuts at: 0 33 911 989 1364 1506 Size: 33 878 78 375 142 Fragments arranged by size: 878 375 142 78 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 990 1506 Size: 990 516 TatI w'GTAC_w Cuts at: 0 171 356 1506 Size: 171 185 1150 Fragments arranged by size: 1150 185 171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 987 1506 Size: 987 519 XmaI C'CCGG_G Cuts at: 0 1237 1506 Size: 1237 269 Enzymes that do cut: AcuI AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI BtgI BtgZI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI PspOMI PsrI PstI SapI SfcI SfoI SmaI SnaBI SphI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI BglII BmrI BplI BpuEI BsaBI BsaWI BsaXI BsmI BsmBI BspEI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI MspA1I NdeI NotI NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI