################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 41 TCCGGA T^CCGGA AcyI 278 GACGCC G[GA]^CG[CT]C AfaI 97 GTAC GT^AC AocI 805 CCTGAGG CC^T[ACGT]AGG BbvCI 875 CCTCAGC CCTCAGC(-5/-2) BbvI 50 GCAGC GCAGC(8/12) BciVI 44 GGATAC GTATCC(6/5) BsaHI 278 GACGCC G[GA]^CG[CT]C BsaWI 41 TCCGGA [AT]^CCGG[AT] BseAI 41 TCCGGA T^CCGGA BseRI 106 CTCCTC GAGGAG(10/8) BspEI 41 TCCGGA T^CCGGA BstYI 828 GGATCT [GA]^GATC[CT] Bsu36I 805 CCTGAGG CC^T[ACGT]AGG DsaI 611 CCATGG C^C[GA][CT]GG EarI 667 CTCTTC CTCTTC(1/4) Eco81I 805 CCTGAGG CC^T[ACGT]AGG EcoNI 480 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 50 GCAGC GC^[ACGT]GC HgaI 278 GACGC GACGC(5/10) Hin1I 278 GACGCC G[GA]^CG[CT]C ItaI 50 GCAGC GC^[ACGT]GC Ksp632I 667 CTCTTC CTCTTC(1/4) MflI 828 GGATCT [GA]^GATC[CT] MroI 41 TCCGGA T^CCGGA MspA1I 781 CAGCTG C[AC]G^C[GT]G NcoI 611 CCATGG C^CATGG PflMI 780 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 551 GAGTC GAGTC(4/5) PvuII 781 CAGCTG CAG^CTG RsaI 97 GTAC GT^AC SfcI 376 CTACAG C^T[GA][CT]AG SmaI 647 CCCGGG CCC^GGG TseI 50 GCAGC G^C[AT]GC Van91I 780 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 302 CCATTTACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 828 GGATCT [GA]^GATC[CT] XmaCI 647 CCCGGG C^CCGGG XmaI 647 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaI 601 CTCGGG C^[CT]CG[GA]G 647 CCCGGG AvaII 651 GGACC G^G[AT]CC 691 GGTCC BalI 260 TGGCCA TGG^CCA 524 TGGCCA BanI 616 GGTGCC G^G[CT][GA]CC 729 GGTGCC BbsI 4 GTCTTC GAAGAC(2/6) 700 GTCTTC BpmI 565 CTGGAG CTGGAG(16/14) 889 CTGGAG BpuAI 4 GTCTTC GAAGAC(2/6) 700 GTCTTC BsaAI 211 TACGTG [CT]AC^GT[GA] 676 TACGTG BsaBI 201 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 585 GATGCTGATC BsgI 796 CTGCAC GTGCAG(16/14) 316 GTGCAG BsmBI 225 CGTCTC CGTCTC(1/5) 664 CGTCTC BsoBI 601 CTCGGG C^[CT]CG[GA]G 647 CCCGGG BstXI 170 CCATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 206 CCATCTACGTGG MamI 201 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 585 GATGCTGATC MluNI 260 TGGCCA TGG^CCA 524 TGGCCA MscI 260 TGGCCA TGG^CCA 524 TGGCCA MwoI 331 GCTTTCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 340 GCTTCGGGAGC PpuMI 650 GGGACCT [GA]G^G[AT]CC[CT] 690 GGGTCCT TfiI 243 GAATC G^A[AT]TC 630 GATTC Tsp45I 136 GTCAC ^GT[GC]AC 406 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwI AlwNI ApaI BanII BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI FokI HaeIII HapII HinfI HpaII HphI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TspRI