(Circular) (Six-base) MAPSORT of: pFN21AE6267.seq Check: 1565 from: 1 to: 5608 pFN21AE6267 5608 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2692 2721 3671 5055 5323 1632 Size: 1060 29 950 1384 268 1917 Fragments arranged by size: 1917 1384 1060 950 268 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 2681 1166 Size: 1515 4093 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2525 1184 Size: 1341 4267 AlwNI CAG_nnn'CTG Cuts at: 2077 2329 2414 2497 2836 2893 3351 4923 2077 Size: 252 85 83 339 57 458 1572 2762 Fragments arranged by size: 2762 1572 458 339 252 85 83 57 ApaI G_GGCC'C Cuts at: 2242 2666 2819 2242 Size: 424 153 5031 Fragments arranged by size: 5031 424 153 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2606 2652 2933 1870 Size: 73 663 46 281 4545 Fragments arranged by size: 4545 663 281 73 46 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2621 2734 2929 4384 618 Size: 325 217 627 834 113 195 1455 1842 Fragments arranged by size: 1842 1455 834 627 325 217 195 113 BanII G_rGCy'C Cuts at: 1391 2242 2526 2666 2819 2927 1391 Size: 851 284 140 153 108 4072 Fragments arranged by size: 4072 851 284 153 140 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2002 2698 928 Size: 971 103 696 3838 Fragments arranged by size: 3838 971 696 103 BbvCI CC'TCA_GC Cuts at: 2881 2881 Size: 5608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2043 3500 4710 2043 Size: 1457 1210 2941 Fragments arranged by size: 2941 1457 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2242 2504 2666 2819 3669 4825 1790 Size: 452 262 162 153 850 1156 2573 Fragments arranged by size: 2573 1156 850 452 262 162 153 BmgBI CAC'GTC Cuts at: 1318 2669 1318 Size: 1351 4257 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2514 3339 4297 Size: 416 53 83 399 24 1097 825 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1097 958 825 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2591 2915 4268 1239 Size: 1352 324 1353 2579 Fragments arranged by size: 2579 1353 1352 324 Bpu10I CC'TnA_GC Cuts at: 2829 2881 2829 Size: 52 5556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 2683 3399 493 Size: 711 1014 465 716 2702 Fragments arranged by size: 2702 1014 716 711 465 BsaBI GATnn'nnATC Cuts at: 2210 2594 5602 2210 Size: 384 3008 2216 Fragments arranged by size: 3008 2216 384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2284 3415 3797 4432 Size: 53 83 186 936 751 1131 382 635 Cuts at: 4432 275 Size: 1451 Fragments arranged by size: 1451 1131 936 751 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2046 4041 4439 4713 4860 5271 2046 Size: 1995 398 274 147 411 2383 Fragments arranged by size: 2383 1995 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 2094 2124 1178 Size: 30 165 30 640 30 21 30 4662 Fragments arranged by size: 4662 640 165 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 1186 Size: 916 4692 BseYI C'CCAG_C Cuts at: 2691 4811 5307 2691 Size: 2120 496 2992 Fragments arranged by size: 2992 2120 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 2786 1163 Size: 573 606 444 3985 Fragments arranged by size: 3985 606 573 444 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3669 3754 4825 3669 Size: 85 1071 4452 Fragments arranged by size: 4452 1071 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 BsmBI CGTCTCn'nnnn_ Cuts at: 2236 2675 2236 Size: 439 5169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2242 2504 2526 2666 2819 2927 3669 Size: 399 452 262 22 140 153 108 742 Cuts at: 3669 3754 4825 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 262 153 140 108 85 22 BspEI T'CCGG_A Cuts at: 2046 5271 2046 Size: 3225 2383 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 844 Size: 2119 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2218 2182 Size: 36 5572 BstYI r'GATC_y Cuts at: 1972 2833 2938 3305 3690 3707 5159 5245 5257 Size: 861 105 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 861 385 367 346 105 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2811 2811 Size: 5608 BtgI C'CryG_G Cuts at: 513 1065 2004 2616 513 Size: 552 939 612 3505 Fragments arranged by size: 3505 939 612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2078 3124 3918 3938 2078 Size: 1046 794 20 3748 Fragments arranged by size: 3748 1046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2265 2529 Size: 54 1425 50 275 18 366 69 264 Cuts at: 2529 3055 3944 5573 8 Size: 526 889 1629 43 Fragments arranged by size: 1629 1425 889 526 366 275 264 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 3538 1353 Size: 1325 860 3423 Fragments arranged by size: 3423 1325 860 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2591 2692 2721 2915 3671 4268 5055 Size: 393 959 101 29 194 756 597 787 Cuts at: 5055 5323 1239 Size: 268 1524 Fragments arranged by size: 1524 959 787 756 597 393 268 194 101 29 EcoNI CCTnn'n_nnAGG Cuts at: 2489 2489 Size: 5608 EcoO109I rG'GnC_Cy Cuts at: 2020 2238 2656 2696 2815 2020 Size: 218 418 40 119 4813 Fragments arranged by size: 4813 418 218 119 40 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 4755 1678 Size: 3077 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2825 2857 4249 4281 4323 Size: 32 135 32 1253 32 1392 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 1392 1253 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2260 2847 4722 4906 1305 Size: 955 587 1875 184 2007 Fragments arranged by size: 2007 1875 955 587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2267 2531 10 Size: 54 1425 343 435 264 3087 Fragments arranged by size: 3087 1425 435 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2318 2507 2681 3566 3925 4084 Size: 648 1050 102 189 174 885 359 159 Cuts at: 4084 518 Size: 2042 Fragments arranged by size: 2042 1050 885 648 359 189 174 159 102 MspA1I CmG'CkG Cuts at: 1606 2788 3024 3701 4849 5094 1606 Size: 1182 236 677 1148 245 2120 Fragments arranged by size: 2120 1182 1148 677 245 236 NcoI C'CATG_G Cuts at: 513 1065 2004 2616 513 Size: 552 939 612 3505 Fragments arranged by size: 3505 939 612 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 2292 1348 Size: 944 4664 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 1108 Size: 1683 3925 PfoI T'CCnGG_A Cuts at: 2484 2535 2484 Size: 51 5557 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2656 2696 2656 Size: 40 5568 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2238 2662 2815 2238 Size: 424 153 5031 Fragments arranged by size: 5031 424 153 PstI C_TGCA'G Cuts at: 830 2960 830 Size: 2130 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2788 2788 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2381 2956 4091 4772 4963 826 Size: 219 1336 575 1135 681 191 1471 Fragments arranged by size: 1471 1336 1135 681 575 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2654 2935 1872 Size: 782 281 4545 Fragments arranged by size: 4545 782 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2421 2616 3047 513 Size: 552 939 115 302 195 431 3074 Fragments arranged by size: 3074 939 552 431 302 195 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 2132 2210 2609 2722 3635 3652 Size: 1255 501 51 78 399 113 913 17 Cuts at: 3652 3805 3990 4435 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 913 501 445 399 185 153 113 78 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2101 3854 5566 Size: 274 80 33 51 1443 124 1753 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1753 1712 1443 274 138 124 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2314 2314 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2652 2933 1870 Size: 782 281 4545 Fragments arranged by size: 4545 782 281 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI