################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 271 GCGG CCGC(-3/-1) AcsI 40 GAATTC [GA]^AATT[CT] AfaI 93 GTAC GT^AC AlwNI 154 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 40 GAATTC [GA]^AATT[CT] AvaII 141 GGACC G^G[AT]CC BcnI 303 CCGGG CC^[GC]GG BmyI 307 GGGCAC G[AGT]GC[ACT]^C BsaI 67 GAGACC GGTCTC(1/5) BsiYI 298 CCTGTCCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 298 CCTGTCCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 115 GAGACG CGTCTC(1/5) Bsp1286I 307 GGGCAC G[AGT]GC[ACT]^C BsrDI 221 GCAATG GCAATG(2/0) BstAPI 25 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 147 GGTCACC G^GT[ACGT]ACC BstF5I 30 GGATG GGATG(2/0) BstPI 147 GGTCACC G^GT[ACGT]ACC DraII 140 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 98 CCGTGG C^C[GA][CT]GG EaeI 272 CGGCCA [CT]^GGCC[GA] EarI 193 GAAGAG CTCTTC(1/4) EcoO109I 140 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 147 GGTCACC G^GT[ACGT]ACC EcoRI 40 GAATTC G^AATTC FokI 30 GGATG GGATG(9/13) HapII 303 CCGG C^CGG HgaI 117 GACGC GACGC(5/10) HinfI 226 GACTC G^A[ACGT]TC HpaII 303 CCGG C^CGG Ksp632I 193 GAAGAG CTCTTC(1/4) MaeIII 148 GTCAC ^GT[ACGT]AC MboII 193 GAAGA GAAGA(8/7) MspI 303 CCGG C^CGG NciI 303 CCGGG CC^[GC]GG NlaIII 171 CATG CATG^ NlaIV 140 GGGACC GG[ACGT]^[ACGT]CC PleI 226 GACTC GAGTC(4/5) PpuMI 140 GGGACCT [GA]G^G[AT]CC[CT] RsaI 93 GTAC GT^AC ScaI 92 AGTACT AGT^ACT SexAI 143 ACCTGGT A^CC[AT]GGT SfaNI 31 GATGC GCATC(5/9) StuI 295 AGGCCT AGG^CCT Tsp45I 148 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AspI 5 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 142 GACCTGGTC BpmI 235 CTGGAG CTGGAG(16/14) 289 CTGGAG BsmAI 67 GAGAC GTCTC(1/5) 115 GAGAC BsmFI 3 GGGAC GGGAC(10/14) 140 GGGAC BsrI 234 ACTGG ACTGG(1/-1) 90 CCAGT Cfr13I 59 GGGCC G^G[ACGT]CC 141 GGACC DdeI 35 CTCAG C^T[ACGT]AG 131 CTGAG HphI 87 TCACC GGTGA(8/7) 149 TCACC MspA1I 157 CAGCTG C[AC]G^C[GT]G 216 CAGCTG PflFI 5 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 142 GACCTGGTC PvuII 157 CAGCTG CAG^CTG 216 CAGCTG Sau96I 59 GGGCC G^G[ACGT]CC 141 GGACC Tsp509I 41 AATT ^AATT 201 AATT TspRI 7 CAGTG CA[GC]TG[ACGT][ACGT]^ 45 CAGTG Tth111I 5 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 142 GACCTGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI ScrFI TseI