##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BpmI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MfeI        MluI        MroI        
MseI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TfiI        Tru9I       TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

ApaI          297  GGGCCC               GGGCC^C
AvaI          325  CTCGGG               C^[CT]CG[GA]G
AvaII         192  GGACC                G^G[AT]CC
BalI          303  TGGCCA               TGG^CCA
BanII         297  GGGCCC               G[GA]GC[CT]^C
BglII         129  AGATCT               A^GATCT
BlpI          219  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      219  GCTGAGC              GC^T[ACGT]AGC
BsaI           37  GAGACC               GGTCTC(1/5)
BseRI         229  GAGGAG               GAGGAG(10/8)
BsmAI          37  GAGAC                GTCTC(1/5)
BsoBI         325  CTCGGG               C^[CT]CG[GA]G
BstYI         129  AGATCT               [GA]^GATC[CT]
CelII         219  GCTGAGC              GC^T[ACGT]AGC
DdeI          220  CTGAG                C^T[ACGT]AG
DpnII         130  GATC                 ^GATC
EaeI          303  TGGCCA               [CT]^GGCC[GA]
EcoRI         106  GAATTC               G^AATTC
HphI          289  GGTGA                GGTGA(8/7)
MaeIII        290  GTGAC                ^GT[ACGT]AC
MboI          130  GATC                 ^GATC
MboII         276  GAAGA                GAAGA(8/7)
MflI          129  AGATCT               [GA]^GATC[CT]
MluNI         303  TGGCCA               TGG^CCA
MscI          303  TGGCCA               TGG^CCA
NdeII         130  GATC                 ^GATC
PpuMI         191  AGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI        130  GATC                 ^GATC
SfaNI         279  GATGC                GCATC(5/9)
SmlI          145  CTCAAG               C^T[CT][GA]AG
StuI          321  AGGCCT               AGG^CCT
TaqI          227  TCGA                 T^CGA
Tsp45I        290  GTGAC                ^GT[GC]AC
TthHB8I       227  TCGA                 T^CGA
XhoII         129  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcsI          106  GAATTC               [GA]^AATT[CT]
              138  AAATTT              
ApoI          106  GAATTC               [GA]^AATT[CT]
              138  AAATTT              
BanI           80  GGCACC               G^G[CT][GA]CC
              334  GGCACC              
BbvI           18  GCAGC                GCAGC(8/12)
              216  GCAGC               
BssSI         271  CACGAG               CACGAG(-5/-1)
              283  CACGAG              
EcoNI          93  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              324  CCTCGGGGAGG         
Fnu4HI         18  GCAGC                GC^[ACGT]GC
              216  GCAGC               
ItaI           18  GCAGC                GC^[ACGT]GC
              216  GCAGC               
MslI          240  CATGCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              283  CACGAGGGTG          
MspA1I         19  CAGCTG               C[AC]G^C[GT]G
              217  CAGCTG              
NlaIII        186  CATG                 CATG^
              240  CATG                
PvuII          19  CAGCTG               CAG^CTG
              217  CAGCTG              
TseI           18  GCAGC                G^C[AT]GC
              216  GCAGC               
Tsp509I       107  AATT                 ^AATT
              139  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       Cfr13I      DraII       
EcoO109I    EcoRII      HaeIII      MnlI        MvaI        
NlaIV       Sau96I      ScrFI