################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MspA1I MunI MvaI MvnI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AciI 242 GCGG CCGC(-3/-1) AcyI 197 GGCGCC G[GA]^CG[CT]C AluI 118 AGCT AG^CT AlwI 206 GATCC GGATC(4/5) AlwNI 82 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 110 GTGCTC G[AT]GC[AT]^C AvaII 182 GGTCC G^G[AT]CC BalI 258 TGGCCA TGG^CCA BanI 197 GGCGCC G^G[CT][GA]CC BbeI 197 GGCGCC GGCGC^C BcnI 122 CCGGG CC^[GC]GG BsaHI 197 GGCGCC G[GA]^CG[CT]C BsiHKAI 110 GTGCTC G[AT]GC[AT]^C BsiYI 215 CCTGTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 215 CCTGTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrFI 194 GCCGGC [GA]^CCGG[CT] BssKI 122 CCGGG ^CC[ACGT]GG BstXI 261 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 205 AGATCC [GA]^GATC[CT] CfoI 198 GCGC GCG^C Cfr10I 194 GCCGGC [GA]^CCGG[CT] DpnII 206 GATC ^GATC DraII 181 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 258 TGGCCA [CT]^GGCC[GA] EarI 99 CTCTTC CTCTTC(1/4) EcoNI 215 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 181 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 261 CCATGG C^C[AT][AT]GG FauI 242 GCGGG CCCGC(4/6) HaeII 197 GGCGCC [GA]GCGC^[CT] HhaI 198 GCGC GCG^C Hin1I 197 GGCGCC G[GA]^CG[CT]C HinP1I 198 GCGC G^CGC HphI 52 TCACC GGTGA(8/7) KasI 197 GGCGCC G^GCGCC Ksp632I 99 CTCTTC CTCTTC(1/4) MaeIII 218 GTGAC ^GT[ACGT]AC MboI 206 GATC ^GATC MboII 100 TCTTC GAAGA(8/7) MflI 205 AGATCC [GA]^GATC[CT] MluNI 258 TGGCCA TGG^CCA MscI 258 TGGCCA TGG^CCA MslI 53 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 194 GCCGGC GCC^GGC NarI 197 GGCGCC GG^CGCC NciI 122 CCGGG CC^[GC]GG NcoI 261 CCATGG C^CATGG NdeII 206 GATC ^GATC NgoMIV 194 GCCGGC G^CCGGC NlaIII 262 CATG CATG^ PpuMI 181 AGGTCCC [GA]G^G[AT]CC[CT] Sau3AI 206 GATC ^GATC ScrFI 122 CCGGG CC^[ACGT]GG SfoI 197 GGCGCC GGC^GCC StyI 261 CCATGG C^C[AT][AT]GG Tsp45I 218 GTGAC ^GT[GC]AC XhoII 205 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BanII 72 GAGCCC G[GA]GC[CT]^C 246 GGGCTC BsrI 41 CCAGT ACTGG(1/-1) 137 CCAGT Cfr13I 133 GGCCC G^G[ACGT]CC 182 GGTCC DdeI 115 CTAAG C^T[ACGT]AG 160 CTGAG DsaI 255 CCGTGG C^C[GA][CT]GG 261 CCATGG HaeIII 133 GGCC GG^CC 259 GGCC HapII 122 CCGG C^CGG 195 CCGG HpaII 122 CCGG C^CGG 195 CCGG MspI 122 CCGG C^CGG 195 CCGG Sau96I 133 GGCCC G^G[ACGT]CC 182 GGTCC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### BbvI BmyI BsaJI BsmFI Bsp1286I Cac8I Fnu4HI ItaI MnlI MwoI NlaIV TseI TspRI