################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tsp45I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 355 GTCGAC GT^[AC][GT]AC AlwI 678 GATCC GGATC(4/5) AlwNI 347 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 52 CCTGAGG CC^T[ACGT]AGG AspI 349 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 727 GGACC G^G[AT]CC BanI 436 GGTGCC G^G[CT][GA]CC BbsI 90 GAAGAC GAAGAC(2/6) BcnI 576 CCGGG CC^[GC]GG BfaI 499 CTAG C^TAG BglI 961 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 442 CTGGAG CTGGAG(16/14) BpuAI 90 GAAGAC GAAGAC(2/6) BseRI 55 GAGGAG GAGGAG(10/8) BsgI 565 GTGCAG GTGCAG(16/14) Bsp1407I 632 TGTACA T^GTACA BsrDI 957 CATTGC GCAATG(2/0) BsrGI 632 TGTACA T^GTACA BstYI 677 AGATCC [GA]^GATC[CT] Bsu36I 52 CCTGAGG CC^T[ACGT]AGG DpnII 678 GATC ^GATC DraII 726 GGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 52 CCTGAGG CC^T[ACGT]AGG EcoO109I 726 GGGACCT [GA]G^G[ACGT]CC[CT] FauI 299 CCCGC CCCGC(4/6) HapII 576 CCGG C^CGG HincII 355 GTCGAC GT[CT]^[GA]AC HindII 355 GTCGAC GT[CT]^[GA]AC HpaII 576 CCGG C^CGG HphI 33 GGTGA GGTGA(8/7) MaeI 499 CTAG C^TAG MaeIII 379 GTTAC ^GT[ACGT]AC MboI 678 GATC ^GATC MflI 677 AGATCC [GA]^GATC[CT] MslI 205 CATACATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 888 CAGCTG C[AC]G^C[GT]G MspI 576 CCGG C^CGG NciI 576 CCGGG CC^[GC]GG NdeII 678 GATC ^GATC PflFI 349 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 726 GGGACCT [GA]G^G[AT]CC[CT] PvuII 888 CAGCTG CAG^CTG SalI 355 GTCGAC G^TCGAC Sau3AI 678 GATC ^GATC SspBI 632 TGTACA T^GTACA TfiI 851 GAATC G^A[AT]TC Tth111I 349 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 245 CCAGTGAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 677 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 300 CCGC CCGC(-3/-1) 829 GCGG AvaI 682 CCCGAG C^[CT]CG[GA]G 719 CCCGAG BalI 740 TGGCCA TGG^CCA 968 TGGCCA BanII 7 GGGCTC G[GA]GC[CT]^C 341 GAGCCC BsmAI 291 GTCTC GTCTC(1/5) 733 GTCTC BsoBI 682 CCCGAG C^[CT]CG[GA]G 719 CCCGAG BstF5I 174 CATCC GGATG(2/0) 123 GGATG Cac8I 751 GCAAGC GC[ACGT]^[ACGT]GC 970 GCCAGC EaeI 740 TGGCCA [CT]^GGCC[GA] 968 TGGCCA FokI 174 CATCC GGATG(9/13) 123 GGATG MluNI 740 TGGCCA TGG^CCA 968 TGGCCA MscI 740 TGGCCA TGG^CCA 968 TGGCCA NdeI 209 CATATG CA^TATG 605 CATATG SfaNI 595 GATGC GCATC(5/9) 748 GATGC Tsp509I 144 AATT ^AATT 879 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI EarI EcoRII Fnu4HI HaeIII HinfI ItaI Ksp632I MboII MnlI MseI MvaI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI SmlI TaqI Tru9I TseI TspRI TthHB8I