(Circular) (Six-base) MAPSORT of: pFN21AE1813.seq Check: 6944 from: 1 to: 5692 pFN21AE1813 5692 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2361 3035 1937 Size: 424 674 4594 Fragments arranged by size: 4594 674 424 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 2616 2729 3755 5139 5407 1632 Size: 554 430 113 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 554 430 268 113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2680 1184 Size: 1496 4196 AlwNI CAG_nnn'CTG Cuts at: 2357 3435 5007 2357 Size: 1078 1572 3042 Fragments arranged by size: 3042 1572 1078 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2687 2724 3017 1870 Size: 73 744 37 293 4545 Fragments arranged by size: 4545 744 293 73 37 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 3013 4468 618 Size: 325 217 627 654 572 1455 1842 Fragments arranged by size: 1842 1455 654 627 572 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2350 3011 1391 Size: 625 334 661 4072 Fragments arranged by size: 4072 661 625 334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2102 928 Size: 971 203 4518 Fragments arranged by size: 4518 971 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2972 4303 136 Size: 107 122 71 631 605 1300 1331 1525 Fragments arranged by size: 1525 1331 1300 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2446 3753 4909 1790 Size: 656 1307 1156 2573 Fragments arranged by size: 2573 1307 1156 656 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2089 2917 3423 Size: 416 53 83 399 24 672 828 506 Cuts at: 3423 4381 442 Size: 958 1753 Fragments arranged by size: 1753 958 828 672 506 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2064 2064 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 4352 1239 Size: 1229 1884 2579 Fragments arranged by size: 2579 1884 1229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 2210 2444 3817 4682 4980 2000 Size: 210 234 1373 865 298 2712 Fragments arranged by size: 2712 1373 865 298 234 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5467 Fragments arranged by size: 5467 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 1186 Size: 889 4803 BseYI C'CCAG_C Cuts at: 2895 4895 5391 2895 Size: 2000 496 3196 Fragments arranged by size: 3196 2000 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 1163 Size: 573 855 4264 Fragments arranged by size: 4264 855 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3753 3838 4909 3753 Size: 85 1071 4536 Fragments arranged by size: 4536 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2350 2446 3011 3753 3838 4909 Size: 399 226 334 96 565 742 85 1071 Cuts at: 4909 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 565 399 334 226 96 85 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3047 844 Size: 2203 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2961 4187 4361 65 Size: 1183 1713 1226 174 1396 Fragments arranged by size: 1713 1396 1226 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4336 4523 1700 Size: 110 2526 187 2869 Fragments arranged by size: 2869 2526 187 110 BsrGI T'GTAC_A Cuts at: 96 2637 96 Size: 2541 3151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstYI r'GATC_y Cuts at: 1972 2682 3022 3389 3774 3791 5243 5329 5341 Size: 710 340 367 385 17 1452 86 12 Cuts at: 5341 5687 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 710 385 367 346 340 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2058 2058 Size: 5692 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 2724 2850 3208 4002 4022 2724 Size: 126 358 794 20 4394 Fragments arranged by size: 4394 794 358 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2938 2973 529 Size: 35 2374 35 3248 Fragments arranged by size: 3248 2374 35 35 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2745 2973 3139 Size: 54 1425 50 275 18 915 228 166 Cuts at: 3139 4028 5657 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 915 889 275 228 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2619 2705 3622 1353 Size: 897 369 86 917 3423 Fragments arranged by size: 3423 917 897 369 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4279 4653 4799 387 Size: 1064 138 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2468 2616 2729 3755 4352 5139 Size: 393 554 282 148 113 1026 597 787 Cuts at: 5139 5407 1239 Size: 268 1524 Fragments arranged by size: 1524 1026 787 597 554 393 282 268 148 113 EcoO109I rG'GnC_Cy Cuts at: 2732 2732 Size: 5692 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2501 2533 2841 Size: 32 135 32 548 32 349 32 308 Cuts at: 2841 2873 2913 2945 4333 4365 4407 4439 1373 Size: 32 40 32 1388 32 42 32 2626 Fragments arranged by size: 2626 1388 548 349 308 135 42 40 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 3036 3282 669 Size: 988 281 424 674 246 3079 Fragments arranged by size: 3079 988 674 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 2975 10 Size: 54 1425 343 915 228 2727 Fragments arranged by size: 2727 1425 915 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 2214 3650 4009 4168 518 Size: 648 1048 1436 359 159 2042 Fragments arranged by size: 2042 1436 1048 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2895 3108 3785 4933 5178 1606 Size: 1289 213 677 1148 245 2120 Fragments arranged by size: 2120 1289 1148 677 245 213 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5140 NdeI CA'TA_TG Cuts at: 387 2215 2611 387 Size: 1828 396 3468 Fragments arranged by size: 3468 1828 396 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 3050 4595 1132 Size: 97 1821 1545 2229 Fragments arranged by size: 2229 1821 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2732 2732 Size: 5692 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PstI C_TGCA'G Cuts at: 830 3044 830 Size: 2214 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2895 2895 Size: 5692 SalI G'TCGA_C Cuts at: 1936 2360 3034 1936 Size: 424 674 4594 Fragments arranged by size: 4594 674 424 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 3040 4175 4856 5047 826 Size: 219 1995 1135 681 191 1471 Fragments arranged by size: 1995 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 2189 2459 3796 4697 4959 Size: 197 926 72 174 270 1337 901 262 Cuts at: 4959 820 Size: 1553 Fragments arranged by size: 1553 1337 926 901 270 262 197 174 72 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 3131 513 Size: 552 2066 3074 Fragments arranged by size: 3074 2066 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 2429 3719 3736 3889 4074 4519 Size: 1255 510 339 1290 17 153 185 445 Cuts at: 4519 325 Size: 1498 Fragments arranged by size: 1498 1290 1255 510 445 339 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 3938 5650 Size: 274 80 33 51 1443 660 1301 1712 Cuts at: 5650 96 Size: 138 Fragments arranged by size: 1712 1443 1301 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5652 5684 5652 Size: 32 5660 Tth111I GACn'n_nGTC Cuts at: 1316 2357 1316 Size: 1041 4651 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2257 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI