(Linear) (Six-base) MAPSORT of: KIEE6570.seq  Check: 4024  from: 1  to: 351



KIEE6570.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     351 
   Size:        116     235
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     351 
   Size:        306      45
 
AvrII C'CTAG_G

Cuts at:      0     129     351 
   Size:        129     222
 
BclI T'GATC_A

Cuts at:      0     282     351 
   Size:        282      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     157     164     351 
   Size:         85      72       7     187

  Fragments arranged by size: 

                 187      85      72       7
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31     351 
   Size:         31     320
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49      61     351 
   Size:         49      12     290

  Fragments arranged by size: 

                 290      49      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     351 
   Size:        272      79
 
BspHI T'CATG_A

Cuts at:      0     161     351 
   Size:        161     190
 
BsrFI r'CCGG_y

Cuts at:      0      40     351 
   Size:         40     311
 
BstYI r'GATC_y

Cuts at:      0     303     351 
   Size:        303      48
 
EaeI y'GGCC_r

Cuts at:      0     215     351 
   Size:        215     136
 
EarI CTCTTCn'nnn_

Cuts at:      0      14      92     160     351 
   Size:         14      78      68     191

  Fragments arranged by size: 

                 191      78      68      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     116     157     164     351 
   Size:         85      31      41       7     187

  Fragments arranged by size: 

                 187      85      41      31       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     267     299     351 
   Size:        267      32      52

  Fragments arranged by size: 

                 267      52      32
 
HindIII A'AGCT_T

Cuts at:      0     340     351 
   Size:        340      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     351 
   Size:        176     175
 
MscI TGG'CCA

Cuts at:      0     217     351 
   Size:        217     134
 
MspA1I CmG'CkG

Cuts at:      0     243     351 
   Size:        243     108
 
PvuII CAG'CTG

Cuts at:      0     243     351 
   Size:        243     108
 
ScaI AGT'ACT

Cuts at:      0     190     351 
   Size:        190     161
 
SexAI A'CCwGG_T

Cuts at:      0     269     351 
   Size:        269      82
 
StyI C'CwwG_G

Cuts at:      0     129     351 
   Size:        129     222
 
TatI w'GTAC_w

Cuts at:      0     188     351 
   Size:        188     163
 
XmnI GAAnn'nnTTC

Cuts at:      0      77     351 
   Size:         77     274
 

 Enzymes that do cut:

     AcuI    AlwNI    AvrII     BclI     BpmI     BsaI    BseRI     BsgI
    BspHI    BsrFI    BstYI     EaeI     EarI  Eco57MI    Hin4I  HindIII
     MmeI     MscI   MspA1I    PvuII     ScaI    SexAI     StyI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI