################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT14I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MfeI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 41 GCGG CCGC(-3/-1) AfaI 510 GTAC GT^AC AluI 478 AGCT AG^CT AlwNI 259 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 261 GGTCC G^G[AT]CC BamHI 659 GGATCC G^GATCC BbvCI 636 CCTCAGC CCTCAGC(-5/-2) BclI 176 TGATCA T^GATCA BglII 737 AGATCT A^GATCT BsaBI 229 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 32 GAGGAG GAGGAG(10/8) BsgI 333 GTGCAG GTGCAG(16/14) BsmAI 524 GAGAC GTCTC(1/5) BsmFI 677 GGGAC GGGAC(10/14) BspMI 627 GCAGGT ACCTGC(4/8) BsrBI 39 GAGCGG CCGCTC(-3/-3) BsrDI 840 CATTGC GCAATG(2/0) BstF5I 278 GGATG GGATG(2/0) Cac8I 41 GCGGGC GC[ACGT]^[ACGT]GC DraII 260 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 327 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 142 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 260 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 582 GAATTC G^AATTC EcoRV 686 GATATC GAT^ATC EcoT22I 247 ATGCAT ATGCA^T FauI 41 GCGGG CCCGC(4/6) FbaI 176 TGATCA T^GATCA FokI 278 GGATG GGATG(9/13) HindIII 477 AAGCTT A^AGCTT MamI 229 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 320 TTAA T^TAA MslI 451 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 247 ATGCAT ATGCA^T PflMI 258 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 510 GTAC GT^AC ScaI 509 AGTACT AGT^ACT SfcI 602 CTGTAG C^T[GA][CT]AG SspI 764 AATATT AAT^ATT Tru9I 320 TTAA T^TAA Van91I 258 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 798 CCATGAAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 292 GAATTT [GA]^AATT[CT] 582 GAATTC ApoI 292 GAATTT [GA]^AATT[CT] 582 GAATTC BalI 444 TGGCCA TGG^CCA 744 TGGCCA BanII 43 GGGCTC G[GA]GC[CT]^C 118 GGGCTC BbvI 348 GCAGC GCAGC(8/12) 62 GCTGC BmyI 43 GGGCTC G[AGT]GC[ACT]^C 118 GGGCTC BsaJI 257 CCCAGG C^C[ACGT][ACGT]GG 483 CCCTGG Bsp1286I 43 GGGCTC G[AGT]GC[ACT]^C 118 GGGCTC Cfr13I 155 GGGCC G^G[ACGT]CC 261 GGTCC DdeI 5 CTAAG C^T[ACGT]AG 637 CTCAG EaeI 444 TGGCCA [CT]^GGCC[GA] 744 TGGCCA EarI 48 CTCTTC CTCTTC(1/4) 708 CTCTTC Fnu4HI 62 GCTGC GC^[ACGT]GC 348 GCAGC ItaI 62 GCTGC GC^[ACGT]GC 348 GCAGC Ksp632I 48 CTCTTC CTCTTC(1/4) 708 CTCTTC MaeIII 212 GTGAC ^GT[ACGT]AC 385 GTCAC MluNI 444 TGGCCA TGG^CCA 744 TGGCCA MscI 444 TGGCCA TGG^CCA 744 TGGCCA PleI 428 GAGTC GAGTC(4/5) 560 GAGTC Sau96I 155 GGGCC G^G[ACGT]CC 261 GGTCC TseI 62 GCTGC G^C[AT]GC 348 GCAGC Tsp45I 212 GTGAC ^GT[GC]AC 385 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AlwI BpmI BsiYI BslI BsrI BssKI BstNI BstYI DpnII EcoRII HaeIII HinfI MboI MboII MflI MnlI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI TaqI TfiI Tsp509I TspRI TthHB8I XhoII