(Circular) (Six-base) MAPSORT of: pFN21AE6645.seq Check: 6824 from: 1 to: 5560 pFN21AE6645 5560 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2197 2355 2421 2840 3623 5007 5275 Size: 537 28 158 66 419 783 1384 268 Cuts at: 5275 1632 Size: 1917 Fragments arranged by size: 1917 1384 783 537 419 268 158 66 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AlwNI CAG_nnn'CTG Cuts at: 2269 3303 4875 2269 Size: 1034 1572 2954 Fragments arranged by size: 2954 1572 1034 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2297 2587 2869 3095 3247 5356 1493 Size: 804 290 282 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 290 282 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2885 1870 Size: 73 942 4545 Fragments arranged by size: 4545 942 73 BamHI G'GATC_C Cuts at: 2664 2890 3257 2664 Size: 226 367 4967 Fragments arranged by size: 4967 367 226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2881 4336 618 Size: 325 217 627 1094 1455 1842 Fragments arranged by size: 1842 1455 1094 627 325 217 BanII G_rGCy'C Cuts at: 1391 2052 2127 2879 1391 Size: 661 75 752 4072 Fragments arranged by size: 4072 752 661 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4589 BbvCI CC'TCA_GC Cuts at: 2642 2642 Size: 5560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 2181 1548 Size: 633 4927 BfrBI ATG'CAT Cuts at: 2254 2254 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2742 5555 2742 Size: 2813 2747 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 3621 4777 1790 Size: 1831 1156 2573 Fragments arranged by size: 2573 1831 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 2291 2613 4220 1239 Size: 910 142 322 1607 2579 Fragments arranged by size: 2579 1607 910 322 142 Bpu10I CC'TnA_GC Cuts at: 2642 2642 Size: 5560 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4223 882 Size: 617 2724 2219 Fragments arranged by size: 2724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 2238 5554 2238 Size: 3316 2244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3367 3749 4384 275 Size: 53 83 186 936 1834 382 635 1451 Fragments arranged by size: 1834 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3993 4391 4665 4812 5223 3993 Size: 398 274 147 411 4330 Fragments arranged by size: 4330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2424 2454 1178 Size: 30 165 30 1021 30 4284 Fragments arranged by size: 4284 1021 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2052 1186 Size: 866 4694 BseYI C'CCAG_C Cuts at: 4763 5259 4763 Size: 496 5064 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2359 1163 Size: 573 623 4364 Fragments arranged by size: 4364 623 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3621 3706 4777 3621 Size: 85 1071 4404 Fragments arranged by size: 4404 1071 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2127 2879 3621 3706 4777 1391 Size: 399 262 75 752 742 85 1071 2174 Fragments arranged by size: 2174 1071 752 742 399 262 85 75 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2623 2915 844 Size: 1779 292 3489 Fragments arranged by size: 3489 1779 292 BsrBI CCG'CTC Cuts at: 1394 1823 2046 3447 1394 Size: 429 223 1401 3507 Fragments arranged by size: 3507 1401 429 223 BsrDI GCAATG_nn' Cuts at: 65 1248 2844 4055 4229 65 Size: 1183 1596 1211 174 1396 Fragments arranged by size: 1596 1396 1211 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4204 4391 1700 Size: 110 2394 187 2869 Fragments arranged by size: 2869 2394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5560 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2599 2664 2742 2890 3257 3642 3659 5111 Size: 627 65 78 148 367 385 17 1452 Cuts at: 5111 5197 5209 5555 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 627 385 367 346 148 86 78 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5008 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 2347 3076 3870 3890 2347 Size: 729 794 20 4017 Fragments arranged by size: 4017 794 729 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 2749 3007 Size: 54 1425 50 275 18 619 300 258 Cuts at: 3007 3896 5525 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 619 300 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2059 2719 3490 1353 Size: 706 660 771 3423 Fragments arranged by size: 3423 771 706 660 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2169 2197 2291 2355 2421 2613 Size: 393 517 20 28 94 64 66 192 Cuts at: 2613 2840 3623 4220 5007 5275 1239 Size: 227 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 517 393 268 227 192 94 66 64 28 20 EcoNI CCTnn'n_nnAGG Cuts at: 2151 2855 2151 Size: 704 4856 EcoO109I rG'GnC_Cy Cuts at: 2266 2266 Size: 5560 EcoRI G'AATT_C Cuts at: 2587 2869 3247 2587 Size: 282 378 4900 Fragments arranged by size: 4900 378 282 EcoRV GAT'ATC Cuts at: 2693 2693 Size: 5560 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 4707 1678 Size: 3029 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 4201 4233 4275 Size: 32 135 32 1084 32 1513 32 42 Cuts at: 4275 4307 1373 Size: 32 2626 Fragments arranged by size: 2626 1513 1084 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 2482 748 Size: 1734 3826 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4674 4858 1305 Size: 3369 184 2007 Fragments arranged by size: 3369 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2451 2751 10 Size: 54 1425 343 619 300 2819 Fragments arranged by size: 2819 1425 619 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 3518 3877 4036 518 Size: 648 1294 1058 359 159 2042 Fragments arranged by size: 2042 1294 1058 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2976 3653 4801 5046 1606 Size: 1370 677 1148 245 2120 Fragments arranged by size: 2120 1370 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5008 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NsiI A_TGCA'T Cuts at: 2256 2256 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2918 4463 1132 Size: 97 1689 1545 2229 Fragments arranged by size: 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 2269 1108 Size: 1161 4399 PfoI T'CCnGG_A Cuts at: 2136 2268 2136 Size: 132 5428 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2266 Size: 5560 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 2573 3130 2573 Size: 557 5003 PstI C_TGCA'G Cuts at: 830 2912 830 Size: 2082 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 2516 3808 5520 2516 Size: 1292 1712 2556 Fragments arranged by size: 2556 1712 1292 SfcI C'TryA_G Cuts at: 826 1045 2607 2908 4043 4724 4915 826 Size: 219 1562 301 1135 681 191 1471 Fragments arranged by size: 1562 1471 1135 681 301 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 2771 3484 5 Size: 47 2719 713 2081 Fragments arranged by size: 2719 2081 713 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5560 StyI C'CwwG_G Cuts at: 513 1065 2999 513 Size: 552 1934 3074 Fragments arranged by size: 3074 1934 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3587 3604 3757 3942 4387 325 Size: 1255 2007 17 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3806 5518 Size: 274 80 33 51 1443 537 1292 1712 Cuts at: 5518 96 Size: 138 Fragments arranged by size: 1712 1443 1292 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2731 2763 5520 5552 2731 Size: 32 2757 32 2739 Fragments arranged by size: 2757 2739 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2810 2810 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 3689 3689 Size: 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI