(Linear) (Six-base) MAPSORT of: KIEE6645.seq Check: 6621 from: 1 to: 855 KIEE6645.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 164 192 350 416 835 855 Size: 164 28 158 66 419 20 Fragments arranged by size: 419 164 158 66 28 20 AlwNI CAG_nnn'CTG Cuts at: 0 264 855 Size: 264 591 ApoI r'AATT_y Cuts at: 0 292 582 855 Size: 292 290 273 Fragments arranged by size: 292 290 273 BamHI G'GATC_C Cuts at: 0 659 855 Size: 659 196 BanII G_rGCy'C Cuts at: 0 47 122 855 Size: 47 75 733 Fragments arranged by size: 733 75 47 BbvCI CC'TCA_GC Cuts at: 0 637 855 Size: 637 218 BclI T'GATC_A Cuts at: 0 176 855 Size: 176 679 BfrBI ATG'CAT Cuts at: 0 249 855 Size: 249 606 BglII A'GATC_T Cuts at: 0 737 855 Size: 737 118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 286 608 855 Size: 144 142 322 247 Fragments arranged by size: 322 247 144 142 Bpu10I CC'TnA_GC Cuts at: 0 637 855 Size: 637 218 BsaBI GATnn'nnATC Cuts at: 0 233 855 Size: 233 622 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 419 449 855 Size: 419 30 406 Fragments arranged by size: 419 406 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 47 855 Size: 47 808 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 354 855 Size: 354 501 Bsp1286I G_dGCh'C Cuts at: 0 47 122 855 Size: 47 75 733 Fragments arranged by size: 733 75 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 618 855 Size: 618 237 BsrBI CCG'CTC Cuts at: 0 41 855 Size: 41 814 BsrDI GCAATG_nn' Cuts at: 0 839 855 Size: 839 16 BstYI r'GATC_y Cuts at: 0 594 659 737 855 Size: 594 65 78 118 Fragments arranged by size: 594 118 78 65 BtsI GCAGTG_nn' Cuts at: 0 342 855 Size: 342 513 DraIII CAC_nnn'GTG Cuts at: 0 332 855 Size: 332 523 EaeI y'GGCC_r Cuts at: 0 444 744 855 Size: 444 300 111 Fragments arranged by size: 444 300 111 EarI CTCTTCn'nnn_ Cuts at: 0 54 714 855 Size: 54 660 141 Fragments arranged by size: 660 141 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 164 192 286 350 416 608 835 Size: 144 20 28 94 64 66 192 227 Cuts at: 835 855 Size: 20 Fragments arranged by size: 227 192 144 94 66 64 28 20 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 146 855 Size: 146 709 EcoO109I rG'GnC_Cy Cuts at: 0 261 855 Size: 261 594 EcoRI G'AATT_C Cuts at: 0 582 855 Size: 582 273 EcoRV GAT'ATC Cuts at: 0 688 855 Size: 688 167 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 651 683 855 Size: 651 32 172 Fragments arranged by size: 651 172 32 HindIII A'AGCT_T Cuts at: 0 477 855 Size: 477 378 MscI TGG'CCA Cuts at: 0 446 746 855 Size: 446 300 109 Fragments arranged by size: 446 300 109 MslI CAynn'nnrTG Cuts at: 0 455 855 Size: 455 400 NsiI A_TGCA'T Cuts at: 0 251 855 Size: 251 604 PflMI CCAn_nnn'nTGG Cuts at: 0 264 855 Size: 264 591 PfoI T'CCnGG_A Cuts at: 0 131 263 855 Size: 131 132 592 Fragments arranged by size: 592 132 131 PpuMI rG'GwC_Cy Cuts at: 0 261 855 Size: 261 594 PsiI TTA'TAA Cuts at: 0 568 855 Size: 568 287 ScaI AGT'ACT Cuts at: 0 511 855 Size: 511 344 SfcI C'TryA_G Cuts at: 0 602 855 Size: 602 253 SspI AAT'ATT Cuts at: 0 766 855 Size: 766 89 TatI w'GTAC_w Cuts at: 0 509 855 Size: 509 346 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 726 758 855 Size: 726 32 97 Fragments arranged by size: 726 97 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 805 855 Size: 805 50 Enzymes that do cut: AcuI AlwNI ApoI BamHI BanII BbvCI BclI BfrBI BglII BpmI Bpu10I BsaBI BsaXI BseRI BsgI Bsp1286I BspMI BsrBI BsrDI BstYI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HindIII MscI MslI NsiI PflMI PfoI PpuMI PsiI ScaI SfcI SspI TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI