Restriction map for
pFN21AE3679
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BseAI BseRI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AcsI 462 AAATTC [GA]^AATT[CT]
AcyI 34 GGCGCC G[GA]^CG[CT]C
AflIII 790 ACATGT A^C[GA][CT]GT
AgeI 53 ACCGGT A^CCGGT
ApoI 462 AAATTC [GA]^AATT[CT]
AspHI 846 GAGCAC G[AT]GC[AT]^C
AvaII 288 GGACC G^G[AT]CC
BanI 34 GGCGCC G^G[CT][GA]CC
BbeI 34 GGCGCC GGCGC^C
BbvI 510 GCTGC GCAGC(8/12)
BcnI 20 CCGGG CC^[GC]GG
BfaI 775 CTAG C^TAG
BmyI 846 GAGCAC G[AGT]GC[ACT]^C
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsgI 511 CTGCAC GTGCAG(16/14)
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsiHKAI 846 GAGCAC G[AT]GC[AT]^C
Bsp1286I 846 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 790 ACATGT A^CATGT
BsrBI 501 GAGCGG CCGCTC(-3/-3)
BsrDI 878 GCAATG GCAATG(2/0)
BsrFI 53 ACCGGT [GA]^CCGG[CT]
BstAPI 460 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 316 GGTCACC G^GT[ACGT]ACC
BstF5I 506 GGATG GGATG(2/0)
BstPI 316 GGTCACC G^GT[ACGT]ACC
Cac8I 862 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 35 GCGC GCG^C
Cfr10I 53 ACCGGT [GA]^CCGG[CT]
DraII 597 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 17 CGGCCG [CT]^GGCC[GA]
EagI 17 CGGCCG C^GGCCG
EclXI 17 CGGCCG C^GGCCG
Eco52I 17 CGGCCG C^GGCCG
EcoO109I 597 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 316 GGTCACC G^GT[ACGT]ACC
FauI 503 GCGGG CCCGC(4/6)
FokI 506 GGATG GGATG(9/13)
HaeII 34 GGCGCC [GA]GCGC^[CT]
HhaI 35 GCGC GCG^C
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HinP1I 35 GCGC G^CGC
KasI 34 GGCGCC G^GCGCC
MaeI 775 CTAG C^TAG
MseI 814 TTAA T^TAA
NarI 34 GGCGCC GG^CGCC
NciI 20 CCGGG CC^[GC]GG
NspI 790 ACATGT [GA]CATG^[CT]
PaeR7I 386 CTCGAG C^TCGAG
PinAI 53 ACCGGT A^CCGGT
PshAI 787 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 608 CAGCTG CAG^CTG
SapI 807 GAAGAGC GCTCTTC(1/4)
SfcI 41 CTGTAG C^T[GA][CT]AG
SfoI 34 GGCGCC GGC^GCC
SmlI 386 CTCGAG C^T[CT][GA]AG
StuI 854 AGGCCT AGG^CCT
TaqI 387 TCGA T^CGA
Tru9I 814 TTAA T^TAA
TseI 510 GCTGC G^C[AT]GC
Tsp509I 463 AATT ^AATT
TthHB8I 387 TCGA T^CGA
XhoI 386 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 234 GGATC GGATC(4/5)
478 GGATC
AvaI 30 CTCGGG C^[CT]CG[GA]G
386 CTCGAG
BglII 382 AGATCT A^GATCT
409 AGATCT
BpmI 413 CTCCAG CTGGAG(16/14)
498 CTGGAG
BsaBI 119 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
377 GATACAGATC
BsaWI 53 ACCGGT [AT]^CCGG[AT]
99 ACCGGA
BsmFI 527 GGGAC GGGAC(10/14)
113 GTCCC
BsoBI 30 CTCGGG C^[CT]CG[GA]G
386 CTCGAG
DsaI 355 CCACGG C^C[GA][CT]GG
769 CCGTGG
HphI 820 GGTGA GGTGA(8/7)
318 TCACC
MamI 119 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
377 GATACAGATC
MspA1I 38 CCGCTG C[AC]G^C[GT]G
608 CAGCTG
NlaIV 34 GGCGCC GG[ACGT]^[ACGT]CC
224 GGGGCC
SfaNI 507 GATGC GCATC(5/9)
556 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsrI BssKI BstNI BstYI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NdeII
NlaIII PleI Sau3AI Sau96I ScrFI
TfiI Tsp45I TspRI XhoII