(Circular) (Six-base) MAPSORT of: pFN21AE3679.seq Check: 9122 from: 1 to: 5617 pFN21AE3679 5617 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3427 278 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2960 1937 Size: 1023 4594 Acc65I G'GTAC_C Cuts at: 2938 2938 Size: 5617 AclI AA'CG_TT Cuts at: 1563 3744 4117 1563 Size: 2181 373 3063 Fragments arranged by size: 3063 2181 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3680 5064 5332 1632 Size: 2048 1384 268 1917 Fragments arranged by size: 2048 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5420 AflIII A'CryG_T Cuts at: 1203 2795 4516 5493 1203 Size: 1592 1721 977 1327 Fragments arranged by size: 1721 1592 1327 977 AgeI A'CCGG_T Cuts at: 2058 4448 2058 Size: 2390 3227 AhdI GACnn_n'nnGTC Cuts at: 4346 4346 Size: 5617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5617 AlwNI CAG_nnn'CTG Cuts at: 3360 4932 3360 Size: 1572 4045 ApaLI G'TGCA_C Cuts at: 3674 4830 3674 Size: 1156 4461 ApoI r'AATT_y Cuts at: 1493 2467 2926 3152 3304 5413 1493 Size: 974 459 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 459 226 152 AseI AT'TA_AT Cuts at: 160 1032 4171 160 Size: 872 3139 1606 Fragments arranged by size: 3139 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2391 2942 1870 Size: 73 92 356 551 4545 Fragments arranged by size: 4545 551 356 92 73 BamHI G'GATC_C Cuts at: 2947 3314 2947 Size: 367 5250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2938 4393 618 Size: 325 217 627 252 899 1455 1842 Fragments arranged by size: 1842 1455 899 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 2936 1391 Size: 1545 4072 BbeI G_GCGC'C Cuts at: 2043 2043 Size: 5617 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4646 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 1903 Size: 34 1871 34 3678 Fragments arranged by size: 3678 1871 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3509 4719 3509 Size: 1210 4407 BclI T'GATC_A Cuts at: 1548 1548 Size: 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4228 136 Size: 107 122 71 631 605 2556 1525 Fragments arranged by size: 2556 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2387 2414 5612 2387 Size: 27 3198 2392 Fragments arranged by size: 3198 2392 27 BlpI GC'TnA_GC Cuts at: 3034 3034 Size: 5617 Bme1580I G_kGCm'C Cuts at: 1790 3678 4834 1790 Size: 1888 1156 2573 Fragments arranged by size: 2573 1888 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5617 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3348 4306 442 Size: 416 53 83 399 24 1931 958 1753 Fragments arranged by size: 1931 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5617 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2403 2524 4277 1239 Size: 1164 121 1753 2579 Fragments arranged by size: 2579 1753 1164 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3742 4607 4905 3742 Size: 865 298 4454 Fragments arranged by size: 4454 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4280 882 Size: 617 2781 2219 Fragments arranged by size: 2781 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3408 493 Size: 711 2204 2702 Fragments arranged by size: 2702 2204 711 BsaBI GATnn'nnATC Cuts at: 2128 2386 5611 2128 Size: 258 3225 2134 Fragments arranged by size: 3225 2134 258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 3424 3806 4441 Size: 53 83 186 936 507 1384 382 635 Cuts at: 4441 275 Size: 1451 Fragments arranged by size: 1451 1384 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2058 2104 4050 4448 4722 4869 5280 2058 Size: 46 1946 398 274 147 411 2395 Fragments arranged by size: 2395 1946 411 398 274 147 46 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2590 2620 1178 Size: 30 165 30 1187 30 4175 Fragments arranged by size: 4175 1187 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5617 BseYI C'CCAG_C Cuts at: 2574 2611 4820 5316 2574 Size: 37 2209 496 2875 Fragments arranged by size: 2875 2209 496 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 1163 Size: 573 765 4279 Fragments arranged by size: 4279 765 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 3067 3828 4856 1540 Size: 275 186 24 1042 761 1028 2301 Fragments arranged by size: 2301 1042 1028 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2855 3678 3763 4834 2855 Size: 823 85 1071 3638 Fragments arranged by size: 3638 1071 823 85 BsmI GAATG_Cn' Cuts at: 3128 3221 3128 Size: 93 5524 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2855 2936 3678 3763 4834 1391 Size: 399 1065 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 1065 742 399 85 81 BspEI T'CCGG_A Cuts at: 5280 5280 Size: 5617 BspHI T'CATG_A Cuts at: 3506 5236 3506 Size: 1730 3887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2972 844 Size: 2128 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2508 3504 1394 Size: 429 685 996 3507 Fragments arranged by size: 3507 996 685 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2890 4112 4286 65 Size: 1183 1642 1222 174 1396 Fragments arranged by size: 1642 1396 1222 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 4261 4448 1700 Size: 110 248 2203 187 2869 Fragments arranged by size: 2869 2203 248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5617 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5617 BssSI C'ACGA_G Cuts at: 3677 4689 3677 Size: 1012 4605 BstAPI GCAn_nnn'nTGC Cuts at: 794 2471 794 Size: 1677 3940 BstBI TT'CG_AA Cuts at: 4421 4421 Size: 5617 BstEII G'GTnAC_C Cuts at: 1184 2321 1184 Size: 1137 4480 BstYI r'GATC_y Cuts at: 1972 2387 2414 2483 2947 3314 3699 3716 5168 Size: 415 27 69 464 367 385 17 1452 Cuts at: 5168 5254 5266 5612 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 464 415 385 367 346 86 69 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2360 2774 513 Size: 552 939 356 414 3356 Fragments arranged by size: 3356 939 552 414 356 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4878 Fragments arranged by size: 4878 683 56 BtsI GCAGTG_nn' Cuts at: 3133 3927 3947 3133 Size: 794 20 4803 Fragments arranged by size: 4803 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5582 DraI TTT'AAA Cuts at: 2921 2921 Size: 5617 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4624 809 Size: 507 3308 1802 Fragments arranged by size: 3308 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3064 3953 Size: 54 1425 50 275 18 192 1042 889 Cuts at: 3953 5582 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1042 889 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 3064 1537 Size: 275 210 1042 4090 Fragments arranged by size: 4090 1042 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 2712 2807 3547 1353 Size: 1290 69 95 740 3423 Fragments arranged by size: 3423 1290 740 95 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2045 4204 4578 4724 387 Size: 1064 138 435 21 2159 374 146 1280 Fragments arranged by size: 2159 1280 1064 435 374 146 138 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2524 3680 4277 5064 5332 1239 Size: 393 771 121 1156 597 787 268 1524 Fragments arranged by size: 1524 1156 787 771 597 393 268 121 EcoO109I rG'GnC_Cy Cuts at: 2603 2603 Size: 5617 EcoRI G'AATT_C Cuts at: 2926 3304 2926 Size: 378 5239 FspI TGC'GCA Cuts at: 4123 4123 Size: 5617 HaeII r_GCGC'y Cuts at: 1678 2043 4764 1678 Size: 365 2721 2531 Fragments arranged by size: 2721 2531 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2620 2641 2652 2673 4258 Size: 32 135 32 1048 21 11 21 1585 Cuts at: 4258 4290 4332 4364 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1585 1048 135 42 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2961 3207 669 Size: 988 281 1023 246 3079 Fragments arranged by size: 3079 1023 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5617 HpaI GTT'AAC Cuts at: 3207 3207 Size: 5617 KasI G'GCGC_C Cuts at: 2039 2039 Size: 5617 KpnI G_GTAC'C Cuts at: 2942 2942 Size: 5617 MfeI C'AATT_G Cuts at: 3216 3216 Size: 5617 MluI A'CGCG_T Cuts at: 5493 5493 Size: 5617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2168 2357 2547 2666 4731 4915 1305 Size: 863 189 190 119 2065 184 2007 Fragments arranged by size: 2065 2007 863 190 189 184 119 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3795 Fragments arranged by size: 3795 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3575 3934 4093 518 Size: 648 2409 359 159 2042 Fragments arranged by size: 2409 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2615 3033 3710 4858 5103 1606 Size: 439 570 418 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 570 439 418 245 NarI GG'CG_CC Cuts at: 2040 2040 Size: 5617 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4126 Fragments arranged by size: 4126 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5617 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5617 NotI GC'GGCC_GC Cuts at: 3064 3064 Size: 5617 NspI r_CATG'y Cuts at: 1132 1229 2799 2975 4520 1132 Size: 97 1570 176 1545 2229 Fragments arranged by size: 2229 1570 1545 176 97 PasI CC'CwG_GG Cuts at: 1348 2528 1348 Size: 1180 4437 PciI A'CATG_T Cuts at: 2795 4516 2795 Size: 1721 3896 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5617 PfoI T'CCnGG_A Cuts at: 2479 2501 2479 Size: 22 5595 PmeI GTTT'AAAC Cuts at: 2921 2921 Size: 5617 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3686 3718 5199 5231 3686 Size: 32 1481 32 4072 Fragments arranged by size: 4072 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2796 1613 Size: 1183 4434 PsiI TTA'TAA Cuts at: 3187 3187 Size: 5617 PstI C_TGCA'G Cuts at: 830 2969 830 Size: 2139 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5617 PvuII CAG'CTG Cuts at: 2615 2615 Size: 5617 SalI G'TCGA_C Cuts at: 1936 2959 1936 Size: 1023 4594 SapI GCTCTTCn'nnn_ Cuts at: 2807 2807 Size: 5617 SbfI CC_TGCA'GG Cuts at: 2969 2969 Size: 5617 ScaI AGT'ACT Cuts at: 3865 5577 3865 Size: 1712 3905 SfcI C'TryA_G Cuts at: 826 1045 2046 2965 4100 4781 4972 826 Size: 219 1001 919 1135 681 191 1471 Fragments arranged by size: 1471 1135 1001 919 681 219 191 SfoI GGC'GCC Cuts at: 2041 2041 Size: 5617 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5617 SmaI CCC'GGG Cuts at: 1872 2944 1872 Size: 1072 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2391 3721 4622 4884 820 Size: 197 926 448 1330 901 262 1553 Fragments arranged by size: 1553 1330 926 901 448 262 197 SnaBI TAC'GTA Cuts at: 493 3408 493 Size: 2915 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5617 SphI G_CATG'C Cuts at: 1132 2975 1132 Size: 1843 3774 SspI AAT'ATT Cuts at: 5 52 3541 5 Size: 47 3489 2081 Fragments arranged by size: 3489 2081 47 StuI AGG'CCT Cuts at: 1517 2861 1517 Size: 1344 4273 StyI C'CwwG_G Cuts at: 513 1065 2004 3056 513 Size: 552 939 1052 3074 Fragments arranged by size: 3074 1052 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2919 3644 3661 3814 3999 4444 325 Size: 1255 1339 725 17 153 185 445 1498 Fragments arranged by size: 1498 1339 1255 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3863 5575 96 Size: 274 80 33 51 1443 1886 1712 138 Fragments arranged by size: 1886 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 2590 2622 2623 2655 5577 5609 2483 Size: 32 75 32 1 32 2922 32 2491 Fragments arranged by size: 2922 2491 75 32 32 32 32 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5617 XbaI T'CTAG_A Cuts at: 2953 2953 Size: 5617 XhoI C'TCGA_G Cuts at: 1943 2391 1943 Size: 448 5169 XmaI C'CCGG_G Cuts at: 1870 2942 1870 Size: 1072 4545 XmnI GAAnn'nnTTC Cuts at: 3746 3746 Size: 5617 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3425 276 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI