Restriction map for
pFN21AE6656
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MseI
MslI MspA1I MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Van91I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 399 GAATTT [GA]^AATT[CT]
AcyI 4 GACGCC G[GA]^CG[CT]C
AlwI 342 GGATC GGATC(4/5)
ApaI 356 GGGCCC GGGCC^C
ApoI 399 GAATTT [GA]^AATT[CT]
AspHI 23 GTGCTC G[AT]GC[AT]^C
BalI 84 TGGCCA TGG^CCA
BanII 356 GGGCCC G[GA]GC[CT]^C
BbsI 447 GTCTTC GAAGAC(2/6)
BpuAI 447 GTCTTC GAAGAC(2/6)
BsaHI 4 GACGCC G[GA]^CG[CT]C
BsiHKAI 23 GTGCTC G[AT]GC[AT]^C
BspHI 395 TCATGA T^CATGA
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrFI 461 GCCGGC [GA]^CCGG[CT]
BssSI 718 CTCGTG CACGAG(-5/-1)
BstAPI 576 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 461 GCCGGC [GA]^CCGG[CT]
DrdI 4 GACGCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 84 TGGCCA [CT]^GGCC[GA]
EcoT14I 159 CCTTGG C^C[AT][AT]GG
HaeII 683 GGCGCT [GA]GCGC^[CT]
HgaI 4 GACGC GACGC(5/10)
Hin1I 4 GACGCC G[GA]^CG[CT]C
HindIII 169 AAGCTT A^AGCTT
MaeII 629 ACGT A^CGT
MluNI 84 TGGCCA TGG^CCA
MscI 84 TGGCCA TGG^CCA
NaeI 461 GCCGGC GCC^GGC
NgoMIV 461 GCCGGC G^CCGGC
PaeR7I 593 CTCGAG C^TCGAG
PstI 94 CTGCAG CTGCA^G
RcaI 395 TCATGA T^CATGA
SfcI 94 CTGCAG C^T[GA][CT]AG
SmaI 38 CCCGGG CCC^GGG
StuI 675 AGGCCT AGG^CCT
StyI 159 CCTTGG C^C[AT][AT]GG
Tsp509I 400 AATT ^AATT
XcmI 150 CCACCTGAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 593 CTCGAG C^TCGAG
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 285 GTAC GT^AC
375 GTAC
AluI 170 AGCT AG^CT
219 AGCT
AspI 298 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
628 GACGTGGTC
AvaI 38 CCCGGG C^[CT]CG[GA]G
593 CTCGAG
AvaII 12 GGTCC G^G[AT]CC
523 GGACC
BbvI 93 GCTGC GCAGC(8/12)
573 GCTGC
BcnI 38 CCCGG CC^[GC]GG
39 CCGGG
BfaI 625 CTAG C^TAG
673 CTAG
BmyI 23 GTGCTC G[AGT]GC[ACT]^C
356 GGGCCC
BsoBI 38 CCCGGG C^[CT]CG[GA]G
593 CTCGAG
Bsp1286I 23 GTGCTC G[AGT]GC[ACT]^C
356 GGGCCC
BstF5I 610 CATCC GGATG(2/0)
729 CATCC
Cac8I 70 GCTTGC GC[ACGT]^[ACGT]GC
461 GCCGGC
CfoI 91 GCGC GCG^C
684 GCGC
DpnII 343 GATC ^GATC
649 GATC
EarI 77 CTCTTC CTCTTC(1/4)
652 CTCTTC
FokI 610 CATCC GGATG(9/13)
729 CATCC
HapII 39 CCGG C^CGG
462 CCGG
HhaI 91 GCGC GCG^C
684 GCGC
HinP1I 91 GCGC G^CGC
684 GCGC
HpaII 39 CCGG C^CGG
462 CCGG
Ksp632I 77 CTCTTC CTCTTC(1/4)
652 CTCTTC
MaeI 625 CTAG C^TAG
673 CTAG
MboI 343 GATC ^GATC
649 GATC
MspI 39 CCGG C^CGG
462 CCGG
NciI 38 CCCGG CC^[GC]GG
39 CCGGG
NdeII 343 GATC ^GATC
649 GATC
PflFI 298 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
628 GACGTGGTC
RsaI 285 GTAC GT^AC
375 GTAC
Sau3AI 343 GATC ^GATC
649 GATC
TseI 93 GCTGC G^C[AT]GC
573 GCTGC
Tsp45I 266 GTCAC ^GT[GC]AC
296 GTGAC
TspRI 21 CAGTG CA[GC]TG[ACGT][ACGT]^
258 CACTG
Tth111I 298 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
628 GACGTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsrI BssKI BstNI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboII
MnlI MvaI MwoI NlaIII NlaIV
PleI Sau96I ScrFI SfaNI SmlI
TaqI TfiI TthHB8I