################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Asp700I 367 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 473 CCCGAG C^[CT]CG[GA]G AvaII 258 GGACC G^G[AT]CC BalI 233 TGGCCA TGG^CCA BamHI 100 GGATCC G^GATCC BanI 281 GGTGCC G^G[CT][GA]CC BanII 571 GGGCTC G[GA]GC[CT]^C BclI 15 TGATCA T^GATCA BpmI 463 CTGGAG CTGGAG(16/14) BsiYI 90 CCGACCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 90 CCGACCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 473 CCCGAG C^[CT]CG[GA]G BspMI 260 ACCTGC ACCTGC(4/8) BstEII 85 GGTGACC G^GT[ACGT]ACC BstPI 85 GGTGACC G^GT[ACGT]ACC CfoI 315 GCGC GCG^C Cfr13I 258 GGACC G^G[ACGT]CC DraII 257 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 233 TGGCCA [CT]^GGCC[GA] EarI 508 CTCTTC CTCTTC(1/4) EcoO109I 257 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 85 GGTGACC G^GT[ACGT]ACC EcoT14I 236 CCATGG C^C[AT][AT]GG EcoT22I 9 ATGCAT ATGCA^T FbaI 15 TGATCA T^GATCA HhaI 315 GCGC GCG^C HinP1I 315 GCGC G^CGC Ksp632I 508 CTCTTC CTCTTC(1/4) MaeIII 86 GTGAC ^GT[ACGT]AC MluNI 233 TGGCCA TGG^CCA MscI 233 TGGCCA TGG^CCA MseI 418 TTAA T^TAA MslI 397 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 236 CCATGG C^CATGG NsiI 9 ATGCAT ATGCA^T PleI 299 GACTC GAGTC(4/5) PpuMI 257 AGGACCT [GA]G^G[AT]CC[CT] PstI 262 CTGCAG CTGCA^G Sau96I 258 GGACC G^G[ACGT]CC StyI 236 CCATGG C^C[AT][AT]GG TfiI 114 GATTC G^A[AT]TC Tru9I 418 TTAA T^TAA Tsp45I 86 GTGAC ^GT[GC]AC Tsp509I 420 AATT ^AATT XcmI 434 CCAACTTTACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 367 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 256 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 518 CAGATGCTG BbvCI 539 CCTCAGC CCTCAGC(-5/-2) 34 GCTGAGG BmyI 282 GTGCCC G[AGT]GC[ACT]^C 571 GGGCTC BsmI 7 GAATGC GAATGC(1/-1) 593 GCATTC Bsp1286I 282 GTGCCC G[AGT]GC[ACT]^C 571 GGGCTC BssKI 94 CCTGG ^CC[ACGT]GG 130 CCTGG BstF5I 228 GGATG GGATG(2/0) 468 GGATG BstNI 94 CCTGG CC^[AT]GG 130 CCTGG BstYI 100 GGATCC [GA]^GATC[CT] 146 AGATCC DsaI 236 CCATGG C^C[GA][CT]GG 443 CCGTGG EcoRII 94 CCTGG ^CC[AT]GG 130 CCTGG FokI 228 GGATG GGATG(9/13) 468 GGATG HaeIII 234 GGCC GG^CC 240 GGCC HinfI 114 GATTC G^A[ACGT]TC 299 GACTC HphI 85 GGTGA GGTGA(8/7) 427 GGTGA MflI 100 GGATCC [GA]^GATC[CT] 146 AGATCC MspA1I 191 CAGCTG C[AC]G^C[GT]G 248 CAGCTG MvaI 94 CCTGG CC^[AT]GG 130 CCTGG NlaIII 139 CATG CATG^ 237 CATG PvuII 191 CAGCTG CAG^CTG 248 CAGCTG ScrFI 94 CCTGG CC^[ACGT]GG 130 CCTGG SfcI 53 CTGTAG C^T[GA][CT]AG 262 CTGCAG TaqI 112 TCGA T^CGA 306 TCGA TspRI 377 CAGTG CA[GC]TG[ACGT][ACGT]^ 461 CACTG TthHB8I 112 TCGA T^CGA 306 TCGA XhoII 100 GGATCC [GA]^GATC[CT] 146 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsrI DdeI DpnII Fnu4HI ItaI MboI MboII MnlI MwoI NdeII NlaIV Sau3AI SfaNI TseI