Restriction map for
pFN21AE3718
(Circular) (Six-base) MAPSORT of: pFN21AE3718.seq Check: 3673 from: 1 to: 12118
pFN21AE3718 12118 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2603 3068 3218 3788 7691 8993 2603
Size: 465 150 570 3903 1302 5728
Fragments arranged by size:
5728 3903 1302 570 465 150
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9928 278
Size: 53 83 186 936 8392 2468
Fragments arranged by size:
8392 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5848 7593 9461 1937
Size: 3911 1745 1868 4594
Fragments arranged by size:
4594 3911 1868 1745
Acc65I G'GTAC_C
Cuts at: 2942 5138 6638 6713 9439 2942
Size: 2196 1500 75 2726 5621
Fragments arranged by size:
5621 2726 2196 1500 75
AclI AA'CG_TT
Cuts at: 1563 10245 10618 1563
Size: 8682 373 3063
Fragments arranged by size:
8682 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3359 4696 7161 7338 8306 8892 10181 11565
Size: 1727 1337 2465 177 968 586 1289 1384
Cuts at: 11565 11833 1632
Size: 268 1917
Fragments arranged by size:
2465 1917 1727 1384 1337 1289 968 586
268 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11921
AflIII A'CryG_T
Cuts at: 1203 3440 4091 4689 5006 5120 8478 9378 11017
Size: 2237 651 598 317 114 3358 900 1639
Cuts at: 11017 11994 1203
Size: 977 1327
Fragments arranged by size:
3358 2237 1639 1327 977 900 651 598
317 114
AgeI A'CCGG_T
Cuts at: 10949 10949
Size: 12118
AhdI GACnn_n'nnGTC
Cuts at: 6127 9277 10847 6127
Size: 3150 1570 7398
Fragments arranged by size:
7398 3150 1570
AleI CACnn'nnGTG
Cuts at: 1166 3695 5373 1166
Size: 2529 1678 7911
Fragments arranged by size:
7911 2529 1678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2940 6716 9195 9429 1184
Size: 1756 3776 2479 234 3873
Fragments arranged by size:
3873 3776 2479 1756 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6663 6695 6663
Size: 32 12086
AlwNI CAG_nnn'CTG
Cuts at: 3559 5191 7789 7961 9140 9861 11433 3559
Size: 1632 2598 172 1179 721 1572 4244
Fragments arranged by size:
4244 2598 1632 1572 1179 721 172
ApaI G_GGCC'C
Cuts at: 7296 8265 9008 7296
Size: 969 743 10406
Fragments arranged by size:
10406 969 743
ApaLI G'TGCA_C
Cuts at: 2395 2679 4975 6077 10175 11331 2395
Size: 284 2296 1102 4098 1156 3182
Fragments arranged by size:
4098 3182 2296 1156 1102 284
ApoI r'AATT_y
Cuts at: 1493 4561 9427 9653 9805 11914 1493
Size: 3068 4866 226 152 2109 1697
Fragments arranged by size:
4866 3068 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3936 3993 10672 160
Size: 872 2904 57 6679 1606
Fragments arranged by size:
6679 2904 1606 872 57
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12118
AvaI C'yCGr_G
Cuts at: 1870 1943 3794 4130 5655 5694 6007 6117 6223
Size: 73 1851 336 1525 39 313 110 106
Cuts at: 6223 6306 8058 8142 8154 8895 9443 1870
Size: 83 1752 84 12 741 548 4545
Fragments arranged by size:
4545 1851 1752 1525 741 548 336 313
110 106 84 83 73 39 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3949 3982 4330 4363 4618 4651 5223 5256 6630
Size: 33 348 33 255 33 572 33 1374
Cuts at: 6630 6663 3949
Size: 33 9404
Fragments arranged by size:
9404 1374 572 348 255 33 33 33
33 33
BamHI G'GATC_C
Cuts at: 5060 9448 9815 5060
Size: 4388 367 7363
Fragments arranged by size:
7363 4388 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2591 2705 2942 3317 3806
Size: 325 217 627 804 114 237 375 489
Cuts at: 3806 5138 5288 5603 6027 6250 6638 6713 7601
Size: 1332 150 315 424 223 388 75 888
Cuts at: 7601 8222 8834 9439 10894 618
Size: 621 612 605 1455 1842
Fragments arranged by size:
1842 1455 1332 888 804 627 621 612
605 489 424 388 375 325 315 237
223 217 150 114 75
BanII G_rGCy'C
Cuts at: 1391 4988 7296 8265 8904 8968 9008 9437 1391
Size: 3597 2308 969 639 64 40 429 4072
Fragments arranged by size:
4072 3597 2308 969 639 429 64 40
BbeI G_GCGC'C
Cuts at: 3321 3810 3321
Size: 489 11629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 7771 8129 928
Size: 971 5872 358 4917
Fragments arranged by size:
5872 4917 971 358
BbvCI CC'TCA_GC
Cuts at: 6177 8279 8829 6177
Size: 2102 550 9466
Fragments arranged by size:
9466 2102 550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5444 5478 6445 6479 10309
Size: 34 50 34 3423 34 967 34 3830
Cuts at: 10309 10343 1903
Size: 34 3678
Fragments arranged by size:
3830 3678 3423 967 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2144 2377 3977 4331 5507 10010 11220 2144
Size: 233 1600 354 1176 4503 1210 3042
Fragments arranged by size:
4503 3042 1600 1210 1176 354 233
BclI T'GATC_A
Cuts at: 1548 7893 1548
Size: 6345 5773
BfrBI ATG'CAT
Cuts at: 2078 3137 3141 2078
Size: 1059 4 11055
Fragments arranged by size:
11055 1059 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2355 2520 2637
Size: 107 122 71 631 605 683 165 117
Cuts at: 2637 3219 3909 5163 5421 5649 7485 7865 8220
Size: 582 690 1254 258 228 1836 380 355
Cuts at: 8220 8369 10729 136
Size: 149 2360 1525
Fragments arranged by size:
2360 1836 1525 1254 690 683 631 605
582 380 355 258 228 165 149 122
117 107 71
BglII A'GATC_T
Cuts at: 8270 12113 8270
Size: 3843 8275
BlpI GC'TnA_GC
Cuts at: 2375 9198 9535 2375
Size: 6823 337 4958
Fragments arranged by size:
6823 4958 337
Bme1580I G_kGCm'C
Cuts at: 1790 2399 2633 2676 2683 3098 3169 4979 5045
Size: 609 234 43 7 415 71 1810 66
Cuts at: 5045 5307 5417 5695 6081 6214 6709 7296 8265
Size: 262 110 278 386 133 495 587 969
Cuts at: 8265 8837 9008 10179 11335 1790
Size: 572 171 1171 1156 2573
Fragments arranged by size:
2573 1810 1171 1156 969 609 587 572
495 415 386 278 262 234 171 133
110 71 66 43 7
BmgBI CAC'GTC
Cuts at: 1318 3915 5302 1318
Size: 2597 1387 8134
Fragments arranged by size:
8134 2597 1387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4839 5181
Size: 416 53 83 399 24 2171 1251 342
Cuts at: 5181 5254 5439 6105 6719 7285 7960 8316 8573
Size: 73 185 666 614 566 675 356 257
Cuts at: 8573 8741 8785 9045 9204 9849 10807 442
Size: 168 44 260 159 645 958 1753
Fragments arranged by size:
2171 1753 1251 958 675 666 645 614
566 416 399 356 342 260 257 185
168 159 83 73 53 44 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4081 4654 5827 4081
Size: 573 1173 10372
Fragments arranged by size:
10372 1173 573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3013 3033 4230 5317 6665 7005 7595 8202
Size: 1774 20 1197 1087 1348 340 590 607
Cuts at: 8202 8685 8808 8976 9212 9369 10778 1239
Size: 483 123 168 236 157 1409 2579
Fragments arranged by size:
2579 1774 1409 1348 1197 1087 607 590
483 340 236 168 157 123 20
Bpu10I CC'TnA_GC
Cuts at: 4178 6177 8279 8829 4178
Size: 1999 2102 550 7467
Fragments arranged by size:
7467 2102 1999 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 8746 10243 11108 11406 8746
Size: 1497 865 298 9458
Fragments arranged by size:
9458 1497 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2193 3119 4220 4667 5217 8780 10781
Size: 617 694 926 1101 447 550 3563 2001
Cuts at: 10781 882
Size: 2219
Fragments arranged by size:
3563 2219 2001 1101 926 694 617 550
447
BsaAI yAC'GTr
Cuts at: 493 1204 2271 8481 9909 493
Size: 711 1067 6210 1428 2702
Fragments arranged by size:
6210 2702 1428 1067 711
BsaBI GATnn'nnATC
Cuts at: 3371 12112 3371
Size: 8741 3377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3318 3807 6315 9925
Size: 53 83 186 936 1785 489 2508 3610
Cuts at: 9925 10307 10942 275
Size: 382 635 1451
Fragments arranged by size:
3610 2508 1785 1451 936 635 489 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 7626 10551 10949 11223 11370 11781 7626
Size: 2925 398 274 147 411 7963
Fragments arranged by size:
7963 2925 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 2758 2788 5425
Size: 30 165 30 1083 30 242 30 2637
Cuts at: 5425 5455 6389 6419 6663 6693 8488 8518 8974
Size: 30 934 30 244 30 1795 30 456
Cuts at: 8974 9004 1178
Size: 30 4292
Fragments arranged by size:
4292 2637 1795 1083 934 456 244 242
165 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3722 4745 5459 6394 6593 7028 7422 7533
Size: 2536 1023 714 935 199 435 394 111
Cuts at: 7533 8463 9366 1186
Size: 930 903 3938
Fragments arranged by size:
3938 2536 1023 935 930 903 714 435
394 199 111
BseYI C'CCAG_C
Cuts at: 2257 4148 4248 5774 6257 6420 7087 7296 8589
Size: 1891 100 1526 483 163 667 209 1293
Cuts at: 8589 8752 8970 9081 9115 9133 11321 11817 2257
Size: 163 218 111 34 18 2188 496 2558
Fragments arranged by size:
2558 2188 1891 1526 1293 667 496 483
218 209 163 163 111 100 34 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2872 3223 6552 7474 7631 7725 1163
Size: 573 1136 351 3329 922 157 94 5556
Fragments arranged by size:
5556 3329 1136 922 573 351 157 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 6465 9568 10329 11357 1540
Size: 275 186 1069 3395 3103 761 1028 2301
Fragments arranged by size:
3395 3103 2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2399 2683 2771 4591 4979 4988 5639 6081 10179
Size: 284 88 1820 388 9 651 442 4098
Cuts at: 10179 10264 11335 2399
Size: 85 1071 3182
Fragments arranged by size:
4098 3182 1820 1071 651 442 388 284
88 85 9
BsmI GAATG_Cn'
Cuts at: 2912 3674 4178 4289 4540 5093 6494 7921 9629
Size: 762 504 111 251 553 1401 1427 1708
Cuts at: 9629 9722 2912
Size: 93 5308
Fragments arranged by size:
5308 1708 1427 1401 762 553 504 251
111 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7694 7694
Size: 12118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2399 2633 2676 2683 2771 3098 3169
Size: 399 609 234 43 7 88 327 71
Cuts at: 3169 4591 4979 4988 5045 5307 5417 5639 5695
Size: 1422 388 9 57 262 110 222 56
Cuts at: 5695 6081 6214 6709 7296 8265 8837 8904 8968
Size: 386 133 495 587 969 572 67 64
Cuts at: 8968 9008 9437 10179 10264 11335 1391
Size: 40 429 742 85 1071 2174
Fragments arranged by size:
2174 1422 1071 969 742 609 587 572
495 429 399 388 386 327 262 234
222 133 110 88 85 71 67 64
57 56 43 40 9 7
BspEI T'CCGG_A
Cuts at: 11781 11781
Size: 12118
BspHI T'CATG_A
Cuts at: 8617 10007 11737 8617
Size: 1390 1730 8998
Fragments arranged by size:
8998 1730 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 2613 3068 3101 3218 3788 4439 4901
Size: 1759 10 455 33 117 570 651 462
Cuts at: 4901 6741 7691 8993 9085 9397 9473 844
Size: 1840 950 1302 92 312 76 3489
Fragments arranged by size:
3489 1840 1759 1302 950 651 570 462
455 312 117 92 76 33 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2024 10005 1394
Size: 429 201 7981 3507
Fragments arranged by size:
7981 3507 429 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 2078 2965 5704 10613 10787 65
Size: 1183 830 887 2739 4909 174 1396
Fragments arranged by size:
4909 2739 1396 1183 887 830 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5898 10762 10949 1700
Size: 110 4088 4864 187 2869
Fragments arranged by size:
4864 4088 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12118
BssSI C'ACGA_G
Cuts at: 10178 11190 10178
Size: 1012 11106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 3153 3849 4188 6076 6510 7766 8378
Size: 1286 1073 696 339 1888 434 1256 612
Cuts at: 8378 794
Size: 4534
Fragments arranged by size:
4534 1888 1286 1256 1073 696 612 434
339
BstBI TT'CG_AA
Cuts at: 4292 10922 4292
Size: 6630 5488
BstEII G'GTnAC_C
Cuts at: 1184 9221 1184
Size: 8037 4081
BstXI CCAn_nnnn'nTGG
Cuts at: 2231 2588 5280 6356 8760 2231
Size: 357 2692 1076 2404 5589
Fragments arranged by size:
5589 2692 2404 1076 357
BstYI r'GATC_y
Cuts at: 1972 5060 6827 8085 8270 8526 9448 9815 10200
Size: 3088 1767 1258 185 256 922 367 385
Cuts at: 10200 10217 11669 11755 11767 12113 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3088 1977 1767 1452 1258 922 385 367
346 256 185 86 17 12
BstZ17I GTA'TAC
Cuts at: 5849 5849
Size: 12118
Bsu36I CC'TnA_GG
Cuts at: 2284 2634 7434 2284
Size: 350 4800 6968
Fragments arranged by size:
6968 4800 350
BtgI C'CryG_G
Cuts at: 513 1065 3264 3745 3970 4195 4657 6378 7367
Size: 552 2199 481 225 225 462 1721 989
Cuts at: 7367 7474 7930 513
Size: 107 456 4701
Fragments arranged by size:
4701 2199 1721 989 552 481 462 456
225 225 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7398 487
Size: 683 56 6172 5207
Fragments arranged by size:
6172 5207 683 56
BtsI GCAGTG_nn'
Cuts at: 2090 2575 3016 3533 3823 5503 6077 6916 7761
Size: 485 441 517 290 1680 574 839 845
Cuts at: 7761 7926 8559 8713 8719 9066 9634 10428 10448
Size: 165 633 154 6 347 568 794 20
Cuts at: 10448 2090
Size: 3760
Fragments arranged by size:
3760 1680 845 839 794 633 574 568
517 485 441 347 290 165 154 20
6
ClaI AT'CG_AT
Cuts at: 1554 3648 1554
Size: 2094 10024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12083
DraI TTT'AAA
Cuts at: 4366 9422 4366
Size: 5056 7062
DraIII CAC_nnn'GTG
Cuts at: 2693 3173 4468 8695 2693
Size: 480 1295 4227 6116
Fragments arranged by size:
6116 4227 1295 480
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9379 11125 809
Size: 507 8063 1746 1802
Fragments arranged by size:
8063 1802 1746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 3267 5896
Size: 54 1425 50 275 18 660 777 2629
Cuts at: 5896 5967 7364 8556 9565 10454 12083 8
Size: 71 1397 1192 1009 889 1629 43
Fragments arranged by size:
2629 1629 1425 1397 1192 1009 889 777
660 275 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9565 1537
Size: 275 7753 4090
Fragments arranged by size:
7753 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5667 7158 7570 7950 8028 10048 1353
Size: 4314 1491 412 380 78 2020 3423
Fragments arranged by size:
4314 3423 2020 1491 412 380 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10705 11079 11225 387
Size: 1064 138 9116 374 146 1280
Fragments arranged by size:
9116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4986 4986
Size: 12118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3013 3033 3359 4230 4696 5317 6665
Size: 393 1381 20 326 871 466 621 1348
Cuts at: 6665 7005 7161 7338 7595 8202 8306 8685 8808
Size: 340 156 177 257 607 104 379 123
Cuts at: 8808 8892 8976 9212 9369 10181 10778 11565 11833
Size: 84 84 236 157 812 597 787 268
Cuts at: 11833 1239
Size: 1524
Fragments arranged by size:
1524 1381 1348 871 812 787 621 607
597 466 393 379 340 326 268 257
236 177 157 156 123 104 84 84
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2776 5111 8363 2776
Size: 2335 3252 6531
Fragments arranged by size:
6531 3252 2335
EcoO109I rG'GnC_Cy
Cuts at: 3353 4136 4890 5659 5699 6975 7292 7293 9005
Size: 783 754 769 40 1276 317 1 1712
Cuts at: 9005 9365 3353
Size: 360 6106
Fragments arranged by size:
6106 1712 1276 783 769 754 360 317
40 1
EcoRI G'AATT_C
Cuts at: 9427 9805 9427
Size: 378 11740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3978 4378 3978
Size: 400 11718
FspI TGC'GCA
Cuts at: 6737 10624 6737
Size: 3887 8231
HaeII r_GCGC'y
Cuts at: 1678 2039 2048 3321 3810 6184 11265 1678
Size: 361 9 1273 489 2374 5081 2531
Fragments arranged by size:
5081 2531 2374 1273 489 361 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5450 5482 6113 6145 8224
Size: 32 135 32 3878 32 631 32 2079
Cuts at: 8224 8256 8262 8294 8483 8515 9263 9295 10759
Size: 32 6 32 189 32 748 32 1464
Cuts at: 10759 10791 10833 10865 1373
Size: 32 42 32 2626
Fragments arranged by size:
3878 2626 2079 1464 748 631 189 135
42 32 32 32 32 32 32 32
32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2192 2842 2986 4909 5551 8232
Size: 988 281 254 650 144 1923 642 2681
Cuts at: 8232 9462 9708 669
Size: 1230 246 3079
Fragments arranged by size:
3079 2681 1923 1230 988 650 642 281
254 246 144
HindIII A'AGCT_T
Cuts at: 748 8722 748
Size: 7974 4144
HpaI GTT'AAC
Cuts at: 9708 9708
Size: 12118
KasI G'GCGC_C
Cuts at: 3317 3806 3317
Size: 489 11629
KpnI G_GTAC'C
Cuts at: 2946 5142 6642 6717 9443 2946
Size: 2196 1500 75 2726 5621
Fragments arranged by size:
5621 2726 2196 1500 75
MfeI C'AATT_G
Cuts at: 9717 9717
Size: 12118
MluI A'CGCG_T
Cuts at: 11994 11994
Size: 12118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5493 5838 8446 8514 11232 11416 1305
Size: 4188 345 2608 68 2718 184 2007
Fragments arranged by size:
4188 2718 2608 2007 345 184 68
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 3269 5969 8558 10
Size: 54 1425 343 660 777 2700 2589 3570
Fragments arranged by size:
3570 2700 2589 1425 777 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2083 2460 3129 3445 3657 3695 5278
Size: 648 917 377 669 316 212 38 1583
Cuts at: 5278 5373 8297 8481 8777 9071 9177 10076 10435
Size: 95 2924 184 296 294 106 899 359
Cuts at: 10435 10594 518
Size: 159 2042
Fragments arranged by size:
2924 2042 1583 917 899 669 648 377
359 316 296 294 212 184 159 106
95 38
MspA1I CmG'CkG
Cuts at: 1606 2220 2859 4119 4152 4609 5148 5154 6039
Size: 614 639 1260 33 457 539 6 885
Cuts at: 6039 6099 6168 6234 6797 7737 7763 7958 8828
Size: 60 69 66 563 940 26 195 870
Cuts at: 8828 8960 8996 9361 9534 10211 11359 11604 1606
Size: 132 36 365 173 677 1148 245 2120
Fragments arranged by size:
2120 1260 1148 940 885 870 677 639
614 563 539 457 365 245 195 173
132 69 66 60 36 33 26 6
NaeI GCC'GGC
Cuts at: 5900 5900
Size: 12118
NarI GG'CG_CC
Cuts at: 3318 3807 3318
Size: 489 11629
NcoI C'CATG_G
Cuts at: 513 1065 3264 3745 3970 4195 4657 6378 7367
Size: 552 2199 481 225 225 462 1721 989
Cuts at: 7367 7930 513
Size: 563 4701
Fragments arranged by size:
4701 2199 1721 989 563 552 481 462
225 225
NdeI CA'TA_TG
Cuts at: 387 2370 8127 387
Size: 1983 5757 4378
Fragments arranged by size:
5757 4378 1983
NgoMIV G'CCGG_C
Cuts at: 5898 5898
Size: 12118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12118
NotI GC'GGCC_GC
Cuts at: 9565 9565
Size: 12118
NsiI A_TGCA'T
Cuts at: 2080 3139 3143 2080
Size: 1059 4 11055
Fragments arranged by size:
11055 1059 4
NspI r_CATG'y
Cuts at: 1132 1229 2078 2367 3094 3141 3293 3444 3454
Size: 97 849 289 727 47 152 151 10
Cuts at: 3454 4095 4693 5010 5496 5980 6513 8020 8167
Size: 641 598 317 486 484 533 1507 147
Cuts at: 8167 8597 9382 9406 9476 11021 1132
Size: 430 785 24 70 1545 2229
Fragments arranged by size:
2229 1545 1507 849 785 727 641 598
533 486 484 430 317 289 152 151
147 97 70 47 24 10
PasI CC'CwG_GG
Cuts at: 1348 4678 5161 5419 6390 6754 8522 9338 1348
Size: 3330 483 258 971 364 1768 816 4128
Fragments arranged by size:
4128 3330 1768 971 816 483 364 258
PciI A'CATG_T
Cuts at: 3440 4091 4689 5006 9378 11017 3440
Size: 651 598 317 4372 1639 4541
Fragments arranged by size:
4541 4372 1639 651 598 317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 8708 1108
Size: 7600 4518
PfoI T'CCnGG_A
Cuts at: 2569 5333 6681 7692 2569
Size: 2764 1348 1011 6995
Fragments arranged by size:
6995 2764 1348 1011
PmeI GTTT'AAAC
Cuts at: 9422 9422
Size: 12118
PmlI CAC'GTG
Cuts at: 2271 2271
Size: 12118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6663 6695 10187 10219 11700 11732 6663
Size: 32 3492 32 1481 32 7049
Fragments arranged by size:
7049 3492 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3353 4136 4890 5699 6975 9365 3353
Size: 783 754 809 1276 2390 6106
Fragments arranged by size:
6106 2390 1276 809 783 754
PshAI GACnn'nnGTC
Cuts at: 1613 2549 1613
Size: 936 11182
PsiI TTA'TAA
Cuts at: 9688 9688
Size: 12118
PspOMI G'GGCC_C
Cuts at: 7292 8261 9004 7292
Size: 969 743 10406
Fragments arranged by size:
10406 969 743
PstI C_TGCA'G
Cuts at: 830 2919 3011 3818 5153 5207 5825 7396 7756
Size: 2089 92 807 1335 54 618 1571 360
Cuts at: 7756 9470 830
Size: 1714 3478
Fragments arranged by size:
3478 2089 1714 1571 1335 807 618 360
92 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12118
PvuII CAG'CTG
Cuts at: 4152 5154 6039 6168 6797 7763 7958 8828 8996
Size: 1002 885 129 629 966 195 870 168
Cuts at: 8996 4152
Size: 7274
Fragments arranged by size:
7274 1002 966 885 870 629 195 168
129
SacI G_AGCT'C
Cuts at: 4988 4988
Size: 12118
SalI G'TCGA_C
Cuts at: 1936 9460 1936
Size: 7524 4594
SanDI GG'GwC_CC
Cuts at: 4136 5699 6975 4136
Size: 1563 1276 9279
Fragments arranged by size:
9279 1563 1276
SapI GCTCTTCn'nnn_
Cuts at: 7950 7950
Size: 12118
SbfI CC_TGCA'GG
Cuts at: 9470 9470
Size: 12118
ScaI AGT'ACT
Cuts at: 2312 2654 3619 10366 12078 2312
Size: 342 965 6747 1712 2352
Fragments arranged by size:
6747 2352 1712 965 342
SexAI A'CCwGG_T
Cuts at: 3876 3876
Size: 12118
SfcI C'TryA_G
Cuts at: 826 1045 2915 3007 3814 4000 5149 5203 5746
Size: 219 1870 92 807 186 1149 54 543
Cuts at: 5746 5821 7392 7752 9466 10601 11282 11473 826
Size: 75 1571 360 1714 1135 681 191 1471
Fragments arranged by size:
1870 1714 1571 1471 1149 1135 807 681
543 360 219 191 186 92 75 54
SfoI GGC'GCC
Cuts at: 3319 3808 3319
Size: 489 11629
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12118
SmaI CCC'GGG
Cuts at: 1872 3796 5696 6009 6119 8060 8144 9445 1872
Size: 1924 1900 313 110 1941 84 1301 4545
Fragments arranged by size:
4545 1941 1924 1900 1301 313 110 84
SmlI C'TyrA_G
Cuts at: 820 1017 1943 8725 10222 11123 11385 820
Size: 197 926 6782 1497 901 262 1553
Fragments arranged by size:
6782 1553 1497 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 9909 493
Size: 9416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12118
SphI G_CATG'C
Cuts at: 1132 2078 3141 3293 3454 8167 8597 9476 1132
Size: 946 1063 152 161 4713 430 879 3774
Fragments arranged by size:
4713 3774 1063 946 879 430 161 152
SspI AAT'ATT
Cuts at: 5 52 2186 2785 4390 10042 5
Size: 47 2134 599 1605 5652 2081
Fragments arranged by size:
5652 2134 2081 1605 599 47
StuI AGG'CCT
Cuts at: 1517 6329 7328 8537 1517
Size: 4812 999 1209 5098
Fragments arranged by size:
5098 4812 1209 999
StyI C'CwwG_G
Cuts at: 513 1065 3264 3745 3970 4195 4423 4657 6378
Size: 552 2199 481 225 225 228 234 1721
Cuts at: 6378 7367 7771 7930 8364 8382 9557 513
Size: 989 404 159 434 18 1175 3074
Fragments arranged by size:
3074 2199 1721 1175 989 552 481 434
404 234 228 225 225 159 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4691 5199 8557 8852 9223 10145 10162
Size: 1255 3111 508 3358 295 371 922 17
Cuts at: 10162 10315 10500 10945 325
Size: 153 185 445 1498
Fragments arranged by size:
3358 3111 1498 1255 922 508 445 371
295 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2652 3044
Size: 274 80 33 51 1443 333 342 392
Cuts at: 3044 3617 4205 4346 8793 10364 12076 96
Size: 573 588 141 4447 1571 1712 138
Fragments arranged by size:
4447 1712 1571 1443 588 573 392 342
333 274 141 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2758 2790 3288 3320 5425 5457 7833 7865 8488
Size: 32 498 32 2105 32 2376 32 623
Cuts at: 8488 8520 8641 8673 8974 9006 12078 12110 2758
Size: 32 121 32 301 32 3072 32 2766
Fragments arranged by size:
3072 2766 2376 2105 623 498 301 121
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3878 4904 6979 7589 1316
Size: 2562 1026 2075 610 5845
Fragments arranged by size:
5845 2562 2075 1026 610
XbaI T'CTAG_A
Cuts at: 7813 8312 9454 7813
Size: 499 1142 10477
Fragments arranged by size:
10477 1142 499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5711 6038 6417 7690 8551 5711
Size: 327 379 1273 861 9278
Fragments arranged by size:
9278 1273 861 379 327
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12118
XmaI C'CCGG_G
Cuts at: 1870 3794 5694 6007 6117 8058 8142 9443 1870
Size: 1924 1900 313 110 1941 84 1301 4545
Fragments arranged by size:
4545 1941 1924 1900 1301 313 110 84
XmnI GAAnn'nnTTC
Cuts at: 4112 4799 5627 10247 4112
Size: 687 828 4620 5983
Fragments arranged by size:
5983 4620 828 687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9926 276
Size: 53 83 186 936 8392 2468
Fragments arranged by size:
8392 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI EcoRV FseI FspAI NruI
PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI