################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccII AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1544 GGTACC G^GTACC AccI 1664 GTCTAC GT^[AC][GT]AC AccIII 830 TCCGGA T^CCGGA AclI 1258 AACGTT AA^CGTT AcyI 1369 GGCGTC G[GA]^CG[CT]C AfaI 1545 GTAC GT^AC AflIII 151 ACGTGT A^C[GA][CT]GT AocI 1126 CCTCAGG CC^T[ACGT]AGG ApaI 908 GGGCCC GGGCC^C Asp718I 1544 GGTACC G^GTACC AvaI 1181 CCCGGG C^[CT]CG[GA]G AvrII 1548 CCTAGG C^CTAGG BalI 1557 TGGCCA TGG^CCA BbsI 898 GAAGAC GAAGAC(2/6) BlnI 1548 CCTAGG C^CTAGG BpuAI 898 GAAGAC GAAGAC(2/6) BsaHI 1369 GGCGTC G[GA]^CG[CT]C BsaWI 830 TCCGGA [AT]^CCGG[AT] BseAI 830 TCCGGA T^CCGGA BsmI 1627 GAATGC GAATGC(1/-1) BsoBI 1181 CCCGGG C^[CT]CG[GA]G BspEI 830 TCCGGA T^CCGGA BsrBI 1508 CCGCTC CCGCTC(-3/-3) BsrDI 683 GCAATG GCAATG(2/0) BsrFI 620 ACCGGC [GA]^CCGG[CT] BstAPI 1478 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1126 CCTCAGG CC^T[ACGT]AGG Cfr10I 620 ACCGGC [GA]^CCGG[CT] DraIII 147 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1294 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 432 GAAGAG CTCTTC(1/4) Eco81I 1126 CCTCAGG CC^T[ACGT]AGG FauI 1493 GCGGG CCCGC(4/6) HgaI 1370 GCGTC GACGC(5/10) Hin1I 1369 GGCGTC G[GA]^CG[CT]C KpnI 1544 GGTACC GGTAC^C Ksp632I 432 GAAGAG CTCTTC(1/4) MluNI 1557 TGGCCA TGG^CCA MroI 830 TCCGGA T^CCGGA MscI 1557 TGGCCA TGG^CCA Psp1406I 1258 AACGTT AA^CGTT RsaI 1545 GTAC GT^AC SacI 718 GAGCTC GAGCT^C SmaI 1181 CCCGGG CCC^GGG SmlI 241 CTCAAG C^T[CT][GA]AG SpeI 46 ACTAGT A^CTAGT SspI 228 AATATT AAT^ATT XmaCI 1181 CCCGGG C^CCGGG XmaI 1181 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 774 GATCC GGATC(4/5) 1026 GATCC AlwNI 391 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 955 CAGTTACTG AspHI 718 GAGCTC G[AT]GC[AT]^C 994 GAGCAC BanI 1528 GGCACC G^G[CT][GA]CC 1544 GGTACC BbrPI 150 CACGTG CAC^GTG 742 CACGTG BcnI 1181 CCCGG CC^[GC]GG 1182 CCGGG BfaI 47 CTAG C^TAG 1549 CTAG BsaBI 267 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1026 GATCCTCATC BsiHKAI 718 GAGCTC G[AT]GC[AT]^C 994 GAGCAC BsmAI 200 GAGAC GTCTC(1/5) 1253 GAGAC BspMI 305 ACCTGC ACCTGC(4/8) 445 ACCTGC BstYI 773 AGATCC [GA]^GATC[CT] 1025 AGATCC CfoI 1517 GCGC GCG^C 1652 GCGC DraII 392 AGGGCCT [GA]G^G[ACGT]CC[CT] 749 AGGCCCT EaeI 347 TGGCCG [CT]^GGCC[GA] 1557 TGGCCA EcoO109I 392 AGGGCCT [GA]G^G[ACGT]CC[CT] 749 AGGCCCT HhaI 1517 GCGC GCG^C 1652 GCGC HinP1I 1517 GCGC G^CGC 1652 GCGC MaeI 47 CTAG C^TAG 1549 CTAG MamI 267 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1026 GATCCTCATC MflI 773 AGATCC [GA]^GATC[CT] 1025 AGATCC NciI 1181 CCCGG CC^[GC]GG 1182 CCGGG NspI 968 ACATGC [GA]CATG^[CT] 1404 GCATGT PflMI 390 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 510 CCACACCATGG PmaCI 150 CACGTG CAC^GTG 742 CACGTG PmlI 150 CACGTG CAC^GTG 742 CACGTG Van91I 390 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 510 CCACACCATGG XcmI 335 CCATAGACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1037 CCAAGAGGGTGGTGG XhoII 773 AGATCC [GA]^GATC[CT] 1025 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BanII BbvI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI