(Circular) (Six-base) MAPSORT of: pFN21AE3960.seq Check: 1798 from: 1 to: 6391 pFN21AE3960 6391 bp With 182 enzymes: SgfI * March 28, 2012 11:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3670 3734 1937 Size: 1733 64 4594 Fragments arranged by size: 4594 1733 64 Acc65I G'GTAC_C Cuts at: 3549 3712 3549 Size: 163 6228 AclI AA'CG_TT Cuts at: 1563 3264 4518 4891 1563 Size: 1701 1254 373 3063 Fragments arranged by size: 3063 1701 1254 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 4454 5838 6106 1632 Size: 1280 1542 1384 268 1917 Fragments arranged by size: 1917 1542 1384 1280 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 2156 5290 6267 1203 Size: 953 3134 977 1327 Fragments arranged by size: 3134 1327 977 953 AgeI A'CCGG_T Cuts at: 5222 5222 Size: 6391 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 2522 2673 1166 Size: 1356 151 4884 Fragments arranged by size: 4884 1356 151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3166 1184 Size: 1982 4409 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3249 3281 3249 Size: 32 6359 AlwNI CAG_nnn'CTG Cuts at: 2401 2965 4134 5706 2401 Size: 564 1169 1572 3086 Fragments arranged by size: 3086 1572 1169 564 ApaI G_GGCC'C Cuts at: 2917 2917 Size: 6391 ApaLI G'TGCA_C Cuts at: 4448 5604 4448 Size: 1156 5235 ApoI r'AATT_y Cuts at: 1493 2926 3165 3614 3700 3926 4078 6187 1493 Size: 1433 239 449 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 1433 449 239 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3186 3716 1870 Size: 73 1243 530 4545 Fragments arranged by size: 4545 1243 530 73 AvrII C'CTAG_G Cuts at: 3553 3553 Size: 6391 BamHI G'GATC_C Cuts at: 3721 4088 3721 Size: 367 6024 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3533 3549 3712 5167 618 Size: 325 217 627 1746 16 163 1455 1842 Fragments arranged by size: 1842 1746 1455 627 325 217 163 16 BanII G_rGCy'C Cuts at: 1391 2178 2727 2917 3187 3422 3710 1391 Size: 787 549 190 270 235 288 4072 Fragments arranged by size: 4072 787 549 288 270 235 190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2910 928 Size: 971 1011 4409 Fragments arranged by size: 4409 1011 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4582 4616 1903 Size: 34 2645 34 3678 Fragments arranged by size: 3678 2645 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4283 5493 4283 Size: 1210 5181 BclI T'GATC_A Cuts at: 1548 1548 Size: 6391 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5002 136 Size: 107 122 71 631 605 3330 1525 Fragments arranged by size: 3330 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6386 6386 Size: 6391 BlpI GC'TnA_GC Cuts at: 3808 3808 Size: 6391 Bme1580I G_kGCm'C Cuts at: 1790 2800 2917 4452 5608 1790 Size: 1010 117 1535 1156 2573 Fragments arranged by size: 2573 1535 1156 1010 117 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3299 4122 5080 Size: 416 53 83 399 24 1882 823 958 Cuts at: 5080 442 Size: 1753 Fragments arranged by size: 1882 1753 958 823 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2192 2393 2780 2786 3003 3123 3478 Size: 944 9 201 387 6 217 120 355 Cuts at: 3478 5051 1239 Size: 1573 2579 Fragments arranged by size: 2579 1573 944 387 355 217 201 120 9 6 Bpu10I CC'TnA_GC Cuts at: 2301 3065 2301 Size: 764 5627 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2231 4516 5381 5679 2231 Size: 2285 865 298 2943 Fragments arranged by size: 2943 2285 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5054 882 Size: 617 3555 2219 Fragments arranged by size: 3555 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2157 2384 2749 4182 493 Size: 711 953 227 365 1433 2702 Fragments arranged by size: 2702 1433 953 711 365 227 BsaBI GATnn'nnATC Cuts at: 2276 3035 6385 2276 Size: 759 3350 2282 Fragments arranged by size: 3350 2282 759 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3375 4198 4580 5215 Size: 53 83 186 936 1842 823 382 635 Cuts at: 5215 275 Size: 1451 Fragments arranged by size: 1842 1451 936 823 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2835 4824 5222 5496 5643 6054 2835 Size: 1989 398 274 147 411 3172 Fragments arranged by size: 3172 1989 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3249 3279 1178 Size: 30 165 30 1846 30 4290 Fragments arranged by size: 4290 1846 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2717 2885 3270 3457 3679 1186 Size: 1531 168 385 187 222 3898 Fragments arranged by size: 3898 1531 385 222 187 168 BseYI C'CCAG_C Cuts at: 2029 2949 5594 6090 2029 Size: 920 2645 496 2330 Fragments arranged by size: 2645 2330 920 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2046 3434 3466 1163 Size: 573 310 1388 32 4088 Fragments arranged by size: 4088 1388 573 310 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3841 4602 5630 1540 Size: 275 186 1840 761 1028 2301 Fragments arranged by size: 2301 1840 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2727 3003 4452 4537 5608 2727 Size: 276 1449 85 1071 3510 Fragments arranged by size: 3510 1449 1071 276 85 BsmI GAATG_Cn' Cuts at: 3638 3902 3995 3638 Size: 264 93 6034 Fragments arranged by size: 6034 264 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2727 2800 2917 3003 3187 3422 Size: 399 388 549 73 117 86 184 235 Cuts at: 3422 3710 4452 4537 5608 1391 Size: 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 549 399 388 288 235 184 117 86 85 73 BspEI T'CCGG_A Cuts at: 2835 6054 2835 Size: 3219 3172 BspHI T'CATG_A Cuts at: 4280 6010 4280 Size: 1730 4661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2459 3746 844 Size: 1475 140 1287 3489 Fragments arranged by size: 3489 1475 1287 140 BsrBI CCG'CTC Cuts at: 1394 1823 3515 4278 1394 Size: 429 1692 763 3507 Fragments arranged by size: 3507 1692 763 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2695 4886 5060 65 Size: 1183 1447 2191 174 1396 Fragments arranged by size: 2191 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2625 5035 5222 1700 Size: 110 815 2410 187 2869 Fragments arranged by size: 2869 2410 815 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 4451 5463 4451 Size: 1012 5379 BstAPI GCAn_nnn'nTGC Cuts at: 794 3489 794 Size: 2695 3696 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6391 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2705 3049 3155 2161 Size: 544 344 106 5397 Fragments arranged by size: 5397 544 344 106 BstYI r'GATC_y Cuts at: 1972 2778 3030 3721 4088 4473 4490 5942 6028 Size: 806 252 691 367 385 17 1452 86 Cuts at: 6028 6040 6386 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 806 691 385 367 346 252 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3132 3132 Size: 6391 BtgI C'CryG_G Cuts at: 513 1065 2316 2520 3154 3565 513 Size: 552 1251 204 634 411 3339 Fragments arranged by size: 3339 1251 634 552 411 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5652 Fragments arranged by size: 5652 683 56 BtsI GCAGTG_nn' Cuts at: 2086 2826 3475 3907 4701 4721 2086 Size: 740 649 432 794 20 3756 Fragments arranged by size: 3756 794 740 649 432 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DraIII CAC_nnn'GTG Cuts at: 2157 2157 Size: 6391 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3305 5398 809 Size: 507 1989 2093 1802 Fragments arranged by size: 2093 1989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 3562 3838 Size: 54 1425 50 275 18 522 1210 276 Cuts at: 3838 4727 6356 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1210 889 522 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3838 1537 Size: 275 2026 4090 Fragments arranged by size: 4090 2026 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 4321 1353 Size: 1079 1889 3423 Fragments arranged by size: 3423 1889 1079 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4978 5352 5498 387 Size: 1064 138 3389 374 146 1280 Fragments arranged by size: 3389 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2725 2725 Size: 6391 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2192 2393 2780 2786 2912 3003 Size: 393 551 9 201 387 6 126 91 Cuts at: 3003 3123 3478 4454 5051 5838 6106 1239 Size: 120 355 976 597 787 268 1524 Fragments arranged by size: 1524 976 787 597 551 393 387 355 268 201 126 120 91 9 6 EcoO109I rG'GnC_Cy Cuts at: 2398 2755 2398 Size: 357 6034 EcoRI G'AATT_C Cuts at: 3700 4078 3700 Size: 378 6013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3139 3139 Size: 6391 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 5538 1678 Size: 3860 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 3670 3702 5032 Size: 32 135 32 632 32 1434 32 1330 Cuts at: 5032 5064 5106 5138 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1434 1330 632 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3735 3981 669 Size: 988 281 1797 246 3079 Fragments arranged by size: 3079 1797 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6391 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KpnI G_GTAC'C Cuts at: 3553 3716 3553 Size: 163 6228 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5505 5689 1305 Size: 4200 184 2007 Fragments arranged by size: 4200 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3564 10 Size: 54 1425 343 1732 2837 Fragments arranged by size: 2837 1732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 2673 2703 4349 4708 4867 518 Size: 648 1356 151 30 1646 359 159 2042 Fragments arranged by size: 2042 1646 1356 648 359 159 151 30 MspA1I CmG'CkG Cuts at: 1606 2170 2828 2899 2938 2953 3015 3498 3807 Size: 564 658 71 39 15 62 483 309 Cuts at: 3807 4484 5632 5877 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 658 564 483 309 245 71 62 39 15 NcoI C'CATG_G Cuts at: 513 1065 2316 2520 3565 513 Size: 552 1251 204 1045 3339 Fragments arranged by size: 3339 1251 1045 552 204 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 2977 3413 3749 5294 1132 Size: 97 1748 436 336 1545 2229 Fragments arranged by size: 2229 1748 1545 436 336 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6391 PciI A'CATG_T Cuts at: 5290 5290 Size: 6391 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 2521 1108 Size: 1293 120 4978 Fragments arranged by size: 4978 1293 120 PfoI T'CCnGG_A Cuts at: 2238 3100 3321 2238 Size: 862 221 5308 Fragments arranged by size: 5308 862 221 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PmlI CAC'GTG Cuts at: 2157 2749 2157 Size: 592 5799 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3249 3281 4460 4492 5973 6005 3249 Size: 32 1179 32 1481 32 3635 Fragments arranged by size: 3635 1481 1179 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PspOMI G'GGCC_C Cuts at: 2913 2913 Size: 6391 PstI C_TGCA'G Cuts at: 830 2265 2593 2937 2943 3743 830 Size: 1435 328 344 6 800 3478 Fragments arranged by size: 3478 1435 800 344 328 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 PvuII CAG'CTG Cuts at: 2170 2828 2899 2938 2953 2170 Size: 658 71 39 15 5608 Fragments arranged by size: 5608 658 71 39 15 SacI G_AGCT'C Cuts at: 2727 2727 Size: 6391 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SfcI C'TryA_G Cuts at: 826 1045 2261 2548 2589 2933 2939 3739 4874 Size: 219 1216 287 41 344 6 800 1135 Cuts at: 4874 5555 5746 826 Size: 681 191 1471 Fragments arranged by size: 1471 1216 1135 800 681 344 287 219 191 41 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3188 3718 1872 Size: 1316 530 4545 Fragments arranged by size: 4545 1316 530 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 4495 5396 5658 820 Size: 197 926 303 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 303 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 2051 152 Size: 1899 4492 SphI G_CATG'C Cuts at: 1132 3749 1132 Size: 2617 3774 SspI AAT'ATT Cuts at: 5 52 2235 4315 5 Size: 47 2183 2080 2081 Fragments arranged by size: 2183 2081 2080 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2316 2520 3363 3553 3565 3830 513 Size: 552 1251 204 843 190 12 265 3074 Fragments arranged by size: 3074 1251 843 552 265 204 190 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2309 3597 4418 4435 4588 4773 5218 Size: 1255 729 1288 821 17 153 185 445 Cuts at: 5218 325 Size: 1498 Fragments arranged by size: 1498 1288 1255 821 729 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4637 6349 96 Size: 274 80 33 51 1443 2660 1712 138 Fragments arranged by size: 2660 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2702 2734 6351 6383 2702 Size: 32 3617 32 2710 Fragments arranged by size: 3617 2710 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6391 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 3049 2347 Size: 702 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3186 3716 1870 Size: 1316 530 4545 Fragments arranged by size: 4545 1316 530 XmnI GAAnn'nnTTC Cuts at: 4520 4520 Size: 6391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI