################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI BamHI BbeI BbrPI BbsI BcgI BclI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MflI MluI MroI MslI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1328 GGTACC G^GTACC AccI 931 GTCTAC GT^[AC][GT]AC AflII 1447 CTTAAG C^TTAAG AlwNI 1273 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1341 GGGCCC GGGCC^C Asp700I 173 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1328 GGTACC G^GTACC AviII 1226 TGCGCA TGC^GCA AvrII 1168 CCTAGG C^CTAGG BbvCI 1129 GCTGAGG CCTCAGC(-5/-2) BfrI 1447 CTTAAG C^TTAAG BglI 1177 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1168 CCTAGG C^CTAGG BsaI 220 GAGACC GGTCTC(1/5) BsaWI 638 ACCGGA [AT]^CCGG[AT] BsmBI 1120 CGTCTC CGTCTC(1/5) BsmI 469 GCATTC GAATGC(1/-1) BsrBI 765 CCGCTC CCGCTC(-3/-3) BsrDI 273 CATTGC GCAATG(2/0) BstBI 48 TTCGAA TT^CGAA BstXI 908 CCAAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG FspI 1226 TGCGCA TGC^GCA HgaI 1119 GCGTC GACGC(5/10) KpnI 1328 GGTACC GGTAC^C MseI 1448 TTAA T^TAA NdeI 913 CATATG CA^TATG NspI 533 ACATGC [GA]CATG^[CT] NspV 48 TTCGAA TT^CGAA PpuMI 496 GGGACCT [GA]G^G[AT]CC[CT] PshAI 89 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 724 GAGCTC GAGCT^C SfiI 1176 GGCCGCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 48 TTCGAA TT^CGAA SmaI 974 CCCGGG CCC^GGG TaqI 49 TCGA T^CGA Tru9I 1448 TTAA T^TAA TthHB8I 49 TCGA T^CGA XcmI 710 CCATCGTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 974 CCCGGG C^CCGGG XmaI 974 CCCGGG C^CCGGG XmnI 173 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 1221 GTAC GT^AC 1329 GTAC AlwI 405 GATCC GGATC(4/5) 281 GGATC AspHI 250 GAGCAC G[AT]GC[AT]^C 724 GAGCTC AvaI 632 CTCGGG C^[CT]CG[GA]G 974 CCCGGG BanI 241 GGCACC G^G[CT][GA]CC 1328 GGTACC BciVI 3 GGATAC GTATCC(6/5) 812 GTATCC BsiHKAI 250 GAGCAC G[AT]GC[AT]^C 724 GAGCTC BsoBI 632 CTCGGG C^[CT]CG[GA]G 974 CCCGGG BspMI 1223 ACCTGC ACCTGC(4/8) 756 GCAGGT CfoI 1002 GCGC GCG^C 1227 GCGC DpnII 282 GATC ^GATC 405 GATC DraII 496 GGGACCT [GA]G^G[ACGT]CC[CT] 1341 GGGCCCC EcoO109I 496 GGGACCT [GA]G^G[ACGT]CC[CT] 1341 GGGCCCC FauI 785 CCCGC CCCGC(4/6) 1339 GCGGG HhaI 1002 GCGC GCG^C 1227 GCGC HinP1I 1002 GCGC G^CGC 1227 GCGC MboI 282 GATC ^GATC 405 GATC NcoI 464 CCATGG C^CATGG 1199 CCATGG NdeII 282 GATC ^GATC 405 GATC PflMI 699 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1251 CCACAGCATGG PvuII 733 CAGCTG CAG^CTG 992 CAGCTG RsaI 1221 GTAC GT^AC 1329 GTAC Sau3AI 282 GATC ^GATC 405 GATC SmlI 1011 CTCAAG C^T[CT][GA]AG 1447 CTTAAG StuI 1100 AGGCCT AGG^CCT 1313 AGGCCT TfiI 10 GAATC G^A[AT]TC 178 GATTC Van91I 699 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1251 CCACAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PstI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI