(Linear) (Six-base) MAPSORT of: KIEE1961.seq Check: 4309 from: 1 to: 1572 KIEE1961.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 747 1572 Size: 747 825 AccI GT'mk_AC Cuts at: 0 932 1572 Size: 932 640 Acc65I G'GTAC_C Cuts at: 0 1328 1572 Size: 1328 244 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1446 1529 1572 Size: 1446 83 43 Fragments arranged by size: 1446 83 43 AflII C'TTAA_G Cuts at: 0 1447 1572 Size: 1447 125 AlwNI CAG_nnn'CTG Cuts at: 0 1278 1572 Size: 1278 294 ApaI G_GGCC'C Cuts at: 0 1345 1572 Size: 1345 227 ApoI r'AATT_y Cuts at: 0 895 1487 1537 1572 Size: 895 592 50 35 Fragments arranged by size: 895 592 50 35 AvaI C'yCGr_G Cuts at: 0 632 974 1572 Size: 632 342 598 Fragments arranged by size: 632 598 342 AvrII C'CTAG_G Cuts at: 0 1168 1572 Size: 1168 404 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 795 828 1320 1353 1572 Size: 795 33 492 33 219 Fragments arranged by size: 795 492 219 33 33 BanI G'GyrC_C Cuts at: 0 241 1328 1572 Size: 241 1087 244 Fragments arranged by size: 1087 244 241 BanII G_rGCy'C Cuts at: 0 164 455 728 1212 1345 1572 Size: 164 291 273 484 133 227 Fragments arranged by size: 484 291 273 227 164 133 BbvCI CC'TCA_GC Cuts at: 0 1130 1572 Size: 1130 442 BciVI GTATCCnnnnn_n' Cuts at: 0 823 1572 Size: 823 749 BglI GCCn_nnn'nGGC Cuts at: 0 1183 1572 Size: 1183 389 Bme1580I G_kGCm'C Cuts at: 0 507 561 1345 1572 Size: 507 54 784 227 Fragments arranged by size: 784 507 227 54 BmrI ACTGGGnnnn_n' Cuts at: 0 222 944 1572 Size: 222 722 628 Fragments arranged by size: 722 628 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 445 742 1108 1198 1572 Size: 176 269 297 366 90 374 Fragments arranged by size: 374 366 297 269 176 90 Bpu10I CC'TnA_GC Cuts at: 0 727 1130 1572 Size: 727 403 442 Fragments arranged by size: 727 442 403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 996 1572 Size: 996 576 BsaI GGTCTCn'nnnn_ Cuts at: 0 214 1572 Size: 214 1358 BsaWI w'CCGG_w Cuts at: 0 638 1572 Size: 638 934 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 469 679 1147 1572 Size: 469 210 468 425 Fragments arranged by size: 469 468 425 210 BsiHKAI G_wGCw'C Cuts at: 0 254 728 1572 Size: 254 474 844 Fragments arranged by size: 844 474 254 BsmI GAATG_Cn' Cuts at: 0 469 1572 Size: 469 1103 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1126 1572 Size: 1126 446 Bsp1286I G_dGCh'C Cuts at: 0 164 254 455 507 561 728 1212 1345 Size: 164 90 201 52 54 167 484 133 Cuts at: 1345 1572 Size: 227 Fragments arranged by size: 484 227 201 167 164 133 90 54 52 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 747 1232 1572 Size: 747 485 340 Fragments arranged by size: 747 485 340 BsrBI CCG'CTC Cuts at: 0 767 1572 Size: 767 805 BsrDI GCAATG_nn' Cuts at: 0 272 1572 Size: 272 1300 BstBI TT'CG_AA Cuts at: 0 49 1572 Size: 49 1523 BstXI CCAn_nnnn'nTGG Cuts at: 0 915 1572 Size: 915 657 BtgI C'CryG_G Cuts at: 0 227 464 538 1199 1572 Size: 227 237 74 661 373 Fragments arranged by size: 661 373 237 227 74 BtsI GCAGTG_nn' Cuts at: 0 1097 1572 Size: 1097 475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1064 1099 1572 Size: 1064 35 473 Fragments arranged by size: 1064 473 35 EaeI y'GGCC_r Cuts at: 0 230 707 917 966 1202 1572 Size: 230 477 210 49 236 370 Fragments arranged by size: 477 370 236 230 210 49 EarI CTCTTCn'nnn_ Cuts at: 0 697 1031 1091 1505 1572 Size: 697 334 60 414 67 Fragments arranged by size: 697 414 334 67 60 EcoICRI GAG'CTC Cuts at: 0 726 1572 Size: 726 846 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 445 742 1108 1198 1446 1529 1572 Size: 176 269 297 366 90 248 83 43 Fragments arranged by size: 366 297 269 248 176 90 83 43 EcoO109I rG'GnC_Cy Cuts at: 0 497 1342 1572 Size: 497 845 230 Fragments arranged by size: 845 497 230 FspI TGC'GCA Cuts at: 0 1228 1572 Size: 1228 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 27 766 798 1513 1545 1572 Size: 27 739 32 715 32 27 Fragments arranged by size: 739 715 32 32 27 27 KpnI G_GTAC'C Cuts at: 0 1332 1572 Size: 1332 240 MscI TGG'CCA Cuts at: 0 709 968 1204 1572 Size: 709 259 236 368 Fragments arranged by size: 709 368 259 236 MspA1I CmG'CkG Cuts at: 0 735 994 1370 1572 Size: 735 259 376 202 Fragments arranged by size: 735 376 259 202 NcoI C'CATG_G Cuts at: 0 464 1199 1572 Size: 464 735 373 Fragments arranged by size: 735 464 373 NdeI CA'TA_TG Cuts at: 0 914 1572 Size: 914 658 NspI r_CATG'y Cuts at: 0 537 1572 Size: 537 1035 PasI CC'CwG_GG Cuts at: 0 1150 1572 Size: 1150 422 PflMI CCAn_nnn'nTGG Cuts at: 0 705 1257 1572 Size: 705 552 315 Fragments arranged by size: 705 552 315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1316 1348 1572 Size: 1316 32 224 Fragments arranged by size: 1316 224 32 PpuMI rG'GwC_Cy Cuts at: 0 497 1572 Size: 497 1075 PshAI GACnn'nnGTC Cuts at: 0 93 1572 Size: 93 1479 PspOMI G'GGCC_C Cuts at: 0 1341 1572 Size: 1341 231 PstI C_TGCA'G Cuts at: 0 740 758 993 1274 1572 Size: 740 18 235 281 298 Fragments arranged by size: 740 298 281 235 18 PvuII CAG'CTG Cuts at: 0 735 994 1572 Size: 735 259 578 Fragments arranged by size: 735 578 259 SacI G_AGCT'C Cuts at: 0 728 1572 Size: 728 844 SfcI C'TryA_G Cuts at: 0 736 754 792 989 1270 1572 Size: 736 18 38 197 281 302 Fragments arranged by size: 736 302 281 197 38 18 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1183 1572 Size: 1183 389 SmaI CCC'GGG Cuts at: 0 976 1572 Size: 976 596 SmlI C'TyrA_G Cuts at: 0 1011 1447 1572 Size: 1011 436 125 Fragments arranged by size: 1011 436 125 StuI AGG'CCT Cuts at: 0 1102 1315 1572 Size: 1102 213 257 Fragments arranged by size: 1102 257 213 StyI C'CwwG_G Cuts at: 0 464 1168 1199 1310 1572 Size: 464 704 31 111 262 Fragments arranged by size: 704 464 262 111 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 244 276 497 529 1572 Size: 244 32 221 32 1043 Fragments arranged by size: 1043 244 221 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 717 1572 Size: 717 855 XmaI C'CCGG_G Cuts at: 0 974 1572 Size: 974 598 XmnI GAAnn'nnTTC Cuts at: 0 177 1572 Size: 177 1395 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AlwNI ApaI ApoI AvaI AvrII BaeI BanI BanII BbvCI BciVI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstBI BstXI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I FspI Hin4I KpnI MscI MspA1I NcoI NdeI NspI PasI PflMI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SfcI SfiI SmaI SmlI StuI StyI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI BamHI BbeI BbsI BcgI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI MslI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI ZraI