################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI KspI MaeII MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 1531 CTTAAG C^TTAAG AspHI 1585 GAGCTC G[AT]GC[AT]^C AvaII 2859 GGACC G^G[AT]CC BanII 1585 GAGCTC G[GA]GC[CT]^C BclI 2481 TGATCA T^GATCA BfrI 1531 CTTAAG C^TTAAG BmyI 1585 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 1585 GAGCTC G[AT]GC[AT]^C BsiYI 167 CCAAAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 167 CCAAAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 1585 GAGCTC G[AGT]GC[ACT]^C BsrDI 2549 CATTGC GCAATG(2/0) BsrI 708 ACTGG ACTGG(1/-1) BstF5I 1670 GGATG GGATG(2/0) DraII 2792 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 1226 CCATGG C^C[GA][CT]GG EcoO109I 2792 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 2016 GATATC GAT^ATC EcoT14I 1226 CCATGG C^C[AT][AT]GG EcoT22I 715 ATGCAT ATGCA^T FauI 2853 GCGGG CCCGC(4/6) FbaI 2481 TGATCA T^GATCA FokI 1670 GGATG GGATG(9/13) HpaI 1452 GTTAAC GTT^AAC HphI 66 GGTGA GGTGA(8/7) NcoI 1226 CCATGG C^CATGG NlaIV 2793 GGCCCC GG[ACGT]^[ACGT]CC NsiI 715 ATGCAT ATGCA^T SacI 1585 GAGCTC GAGCT^C SapI 1741 GAAGAGC GCTCTTC(1/4) ScaI 1006 AGTACT AGT^ACT StuI 1868 AGGCCT AGG^CCT StyI 1226 CCATGG C^C[AT][AT]GG TaqI 2463 TCGA T^CGA Tsp45I 254 GTCAC ^GT[GC]AC TthHB8I 2463 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 64 GCGG CCGC(-3/-1) 2853 GCGG AseI 1642 ATTAAT AT^TAAT 2668 ATTAAT AsnI 1642 ATTAAT AT^TAAT 2668 ATTAAT Asp700I 1687 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1846 GAAGAACTTC BbsI 2571 GAAGAC GAAGAC(2/6) 1406 GTCTTC BglII 574 AGATCT A^GATCT 1500 AGATCT BpuAI 2571 GAAGAC GAAGAC(2/6) 1406 GTCTTC BsaBI 187 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 816 GATCCAAATC BspMI 234 GCAGGT ACCTGC(4/8) 798 GCAGGT Cac8I 690 GCAAGC GC[ACGT]^[ACGT]GC 1898 GCAAGC Cfr13I 2793 GGCCC G^G[ACGT]CC 2859 GGACC EcoRI 291 GAATTC G^AATTC 699 GAATTC HincII 1452 GTTAAC GT[CT]^[GA]AC 2108 GTCAAC HindII 1452 GTTAAC GT[CT]^[GA]AC 2108 GTCAAC MamI 187 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 816 GATCCAAATC MfeI 938 CAATTG C^AATTG 1210 CAATTG MslI 2155 CACCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2767 CATCTTTGTG MunI 938 CAATTG C^AATTG 1210 CAATTG PleI 277 GACTC GAGTC(4/5) 2185 GACTC PshBI 1642 ATTAAT AT^TAAT 2668 ATTAAT PstI 578 CTGCAG CTGCA^G 1152 CTGCAG Sau96I 2793 GGCCC G^G[ACGT]CC 2859 GGACC SfaNI 951 GCATC GCATC(5/9) 2239 GCATC XmnI 1687 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1846 GAAGAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI BbvI BfaI BsaJI BsmAI BssKI BstNI BstYI DdeI DpnII EarI EcoRII Fnu4HI HaeIII HindIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfcI SmlI TfiI Tru9I TseI Tsp509I TspRI XhoII