(Circular) (Six-base) MAPSORT of: pFN21AE3879.seq Check: 4497 from: 1 to: 7633 pFN21AE3879 7633 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5443 278 Size: 53 83 186 936 3907 2468 Fragments arranged by size: 3907 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4976 1937 Size: 3039 4594 Acc65I G'GTAC_C Cuts at: 4954 4954 Size: 7633 AclI AA'CG_TT Cuts at: 1563 5760 6133 1563 Size: 4197 373 3063 Fragments arranged by size: 4197 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 3093 3396 3842 4853 5696 7080 7348 Size: 519 942 303 446 1011 843 1384 268 Cuts at: 7348 1632 Size: 1917 Fragments arranged by size: 1917 1384 1011 942 843 519 446 303 268 AflII C'TTAA_G Cuts at: 820 1017 3536 820 Size: 197 2519 4917 Fragments arranged by size: 4917 2519 197 AflIII A'CryG_T Cuts at: 1203 6532 7509 1203 Size: 5329 977 1327 Fragments arranged by size: 5329 1327 977 AgeI A'CCGG_T Cuts at: 6464 6464 Size: 7633 AhdI GACnn_n'nnGTC Cuts at: 6362 6362 Size: 7633 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7633 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7633 AlwNI CAG_nnn'CTG Cuts at: 2086 2201 2378 2455 5376 6948 2086 Size: 115 177 77 2921 1572 2771 Fragments arranged by size: 2921 2771 1572 177 115 77 ApaLI G'TGCA_C Cuts at: 5690 6846 5690 Size: 1156 6477 ApoI r'AATT_y Cuts at: 1493 2296 2704 3140 3971 4419 4471 4664 4942 Size: 803 408 436 831 448 52 193 278 Cuts at: 4942 5168 5320 7429 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 803 448 436 408 278 226 193 152 52 AseI AT'TA_AT Cuts at: 160 1032 3648 4674 6187 160 Size: 872 2616 1026 1513 1606 Fragments arranged by size: 2616 1606 1513 1026 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7633 AvaI C'yCGr_G Cuts at: 1870 1943 4958 1870 Size: 73 3015 4545 Fragments arranged by size: 4545 3015 73 BamHI G'GATC_C Cuts at: 4963 5330 4963 Size: 367 7266 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4954 6409 618 Size: 325 217 627 3167 1455 1842 Fragments arranged by size: 3167 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3594 4952 1391 Size: 2203 1358 4072 Fragments arranged by size: 4072 2203 1358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3404 4583 928 Size: 971 1505 1179 3978 Fragments arranged by size: 3978 1505 1179 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5824 5858 1903 Size: 34 3887 34 3678 Fragments arranged by size: 3887 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5525 6735 5525 Size: 1210 6423 BclI T'GATC_A Cuts at: 1548 4486 1548 Size: 2938 4695 BfrBI ATG'CAT Cuts at: 2722 2722 Size: 7633 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6244 136 Size: 107 122 71 631 605 4572 1525 Fragments arranged by size: 4572 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2579 3505 7628 2579 Size: 926 4123 2584 Fragments arranged by size: 4123 2584 926 BlpI GC'TnA_GC Cuts at: 5050 5050 Size: 7633 Bme1580I G_kGCm'C Cuts at: 1790 5694 6850 1790 Size: 3904 1156 2573 Fragments arranged by size: 3904 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7633 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5364 6322 442 Size: 416 53 83 399 24 3947 958 1753 Fragments arranged by size: 3947 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7633 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6293 1239 Size: 5054 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2255 3611 5758 6623 6921 2255 Size: 1356 2147 865 298 2967 Fragments arranged by size: 2967 2147 1356 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6296 882 Size: 617 4797 2219 Fragments arranged by size: 4797 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5424 493 Size: 711 4220 2702 Fragments arranged by size: 4220 2702 711 BsaBI GATnn'nnATC Cuts at: 2196 2825 7627 2196 Size: 629 4802 2202 Fragments arranged by size: 4802 2202 629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5440 5822 6457 275 Size: 53 83 186 936 3907 382 635 1451 Fragments arranged by size: 3907 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6066 6464 6738 6885 7296 6066 Size: 398 274 147 411 6403 Fragments arranged by size: 6403 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7408 Fragments arranged by size: 7408 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7633 BseYI C'CCAG_C Cuts at: 6836 7332 6836 Size: 496 7137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7060 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5083 5844 6872 1540 Size: 275 186 3082 761 1028 2301 Fragments arranged by size: 3082 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3594 5694 5779 6850 3594 Size: 2100 85 1071 4377 Fragments arranged by size: 4377 2100 1071 85 BsmI GAATG_Cn' Cuts at: 5144 5237 5144 Size: 93 7540 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3594 4952 5694 5779 6850 1391 Size: 399 1804 1358 742 85 1071 2174 Fragments arranged by size: 2174 1804 1358 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7296 7296 Size: 7633 BspHI T'CATG_A Cuts at: 5522 7252 5522 Size: 1730 5903 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 2794 4988 844 Size: 1386 564 2194 3489 Fragments arranged by size: 3489 2194 1386 564 BsrBI CCG'CTC Cuts at: 1394 1823 5520 1394 Size: 429 3697 3507 Fragments arranged by size: 3697 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4553 6128 6302 65 Size: 1183 3305 1575 174 1396 Fragments arranged by size: 3305 1575 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6277 6464 1700 Size: 110 4467 187 2869 Fragments arranged by size: 4467 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7633 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7633 BssSI C'ACGA_G Cuts at: 5693 6705 5693 Size: 1012 6621 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7633 BstBI TT'CG_AA Cuts at: 6437 6437 Size: 7633 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7633 BstYI r'GATC_y Cuts at: 1972 2197 2579 2641 3505 4823 4963 5330 5715 Size: 225 382 62 864 1318 140 367 385 Cuts at: 5715 5732 7184 7270 7282 7628 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1318 864 385 382 367 346 225 140 86 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3231 513 Size: 552 939 1227 4915 Fragments arranged by size: 4915 1227 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4123 487 Size: 683 56 2897 3997 Fragments arranged by size: 3997 2897 683 56 BtsI GCAGTG_nn' Cuts at: 3559 5149 5943 5963 3559 Size: 1590 794 20 5229 Fragments arranged by size: 5229 1590 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7633 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7598 DraI TTT'AAA Cuts at: 4937 4937 Size: 7633 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6640 809 Size: 507 5324 1802 Fragments arranged by size: 5324 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5080 5969 7598 Size: 54 1425 50 275 18 3250 889 1629 Cuts at: 7598 8 Size: 43 Fragments arranged by size: 3250 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5080 1537 Size: 275 3268 4090 Fragments arranged by size: 4090 3268 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3258 3741 4038 4314 4719 5563 1353 Size: 1905 483 297 276 405 844 3423 Fragments arranged by size: 3423 1905 844 483 405 297 276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6220 6594 6740 387 Size: 1064 138 4631 374 146 1280 Fragments arranged by size: 4631 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3592 3592 Size: 7633 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 3093 3396 3842 4853 5696 6293 Size: 393 519 942 303 446 1011 843 597 Cuts at: 6293 7080 7348 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1011 942 843 787 597 519 446 393 303 268 EcoO109I rG'GnC_Cy Cuts at: 4798 4798 Size: 7633 EcoRI G'AATT_C Cuts at: 2296 2704 4942 5320 2296 Size: 408 2238 378 4609 Fragments arranged by size: 4609 2238 408 378 EcoRV GAT'ATC Cuts at: 4023 4023 Size: 7633 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3317 4052 4298 3317 Size: 735 246 6652 Fragments arranged by size: 6652 735 246 FspI TGC'GCA Cuts at: 6139 6139 Size: 7633 HaeII r_GCGC'y Cuts at: 1678 6780 1678 Size: 5102 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6274 6306 6348 6380 1373 Size: 32 135 32 4702 32 42 32 2626 Fragments arranged by size: 4702 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3459 4115 4977 5223 669 Size: 988 281 1521 656 862 246 3079 Fragments arranged by size: 3079 1521 988 862 656 281 246 HindIII A'AGCT_T Cuts at: 748 3275 3533 3569 748 Size: 2527 258 36 4812 Fragments arranged by size: 4812 2527 258 36 HpaI GTT'AAC Cuts at: 3459 5223 3459 Size: 1764 5869 KpnI G_GTAC'C Cuts at: 4958 4958 Size: 7633 MfeI C'AATT_G Cuts at: 2943 3215 5232 2943 Size: 272 2017 5344 Fragments arranged by size: 5344 2017 272 MluI A'CGCG_T Cuts at: 7509 7509 Size: 7633 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2779 6747 6931 1305 Size: 1474 3968 184 2007 Fragments arranged by size: 3968 2007 1474 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5811 Fragments arranged by size: 5811 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4164 4776 5591 5950 6109 518 Size: 648 2998 612 815 359 159 2042 Fragments arranged by size: 2998 2042 815 648 612 359 159 MspA1I CmG'CkG Cuts at: 1606 5049 5726 6874 7119 1606 Size: 3443 677 1148 245 2120 Fragments arranged by size: 3443 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3231 513 Size: 552 939 1227 4915 Fragments arranged by size: 4915 1227 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7633 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7633 NotI GC'GGCC_GC Cuts at: 5080 5080 Size: 7633 NsiI A_TGCA'T Cuts at: 2724 2724 Size: 7633 NspI r_CATG'y Cuts at: 1132 1229 4991 6536 1132 Size: 97 3762 1545 2229 Fragments arranged by size: 3762 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2086 1348 Size: 738 6895 PciI A'CATG_T Cuts at: 6532 6532 Size: 7633 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7633 PmeI GTTT'AAAC Cuts at: 4937 4937 Size: 7633 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5702 5734 7215 7247 5702 Size: 32 1481 32 6088 Fragments arranged by size: 6088 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7633 PsiI TTA'TAA Cuts at: 4209 5203 4209 Size: 994 6639 PstI C_TGCA'G Cuts at: 830 2587 3161 4985 830 Size: 1757 574 1824 3478 Fragments arranged by size: 3478 1824 1757 574 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7633 SacI G_AGCT'C Cuts at: 3594 3594 Size: 7633 SalI G'TCGA_C Cuts at: 1936 4975 1936 Size: 3039 4594 SapI GCTCTTCn'nnn_ Cuts at: 3741 3741 Size: 7633 SbfI CC_TGCA'GG Cuts at: 4985 4985 Size: 7633 ScaI AGT'ACT Cuts at: 3013 5881 7593 3013 Size: 2868 1712 3053 Fragments arranged by size: 3053 2868 1712 SfcI C'TryA_G Cuts at: 826 1045 2583 3157 4598 4981 6116 6797 6988 Size: 219 1538 574 1441 383 1135 681 191 Cuts at: 6988 826 Size: 1471 Fragments arranged by size: 1538 1471 1441 1135 681 574 383 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7633 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7633 SmaI CCC'GGG Cuts at: 1872 4960 1872 Size: 3088 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 3536 3626 5737 6638 6900 Size: 197 926 291 1302 90 2111 901 262 Cuts at: 6900 820 Size: 1553 Fragments arranged by size: 2111 1553 1302 926 901 291 262 197 90 SnaBI TAC'GTA Cuts at: 493 5424 493 Size: 4931 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7633 SphI G_CATG'C Cuts at: 1132 4991 1132 Size: 3859 3774 SspI AAT'ATT Cuts at: 5 52 5557 5 Size: 47 5505 2081 Fragments arranged by size: 5505 2081 47 StuI AGG'CCT Cuts at: 1517 3875 1517 Size: 2358 5275 StyI C'CwwG_G Cuts at: 513 1065 2004 3231 5072 513 Size: 552 939 1227 1841 3074 Fragments arranged by size: 3074 1841 1227 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5660 5677 5830 6015 6460 325 Size: 1255 4080 17 153 185 445 1498 Fragments arranged by size: 4080 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2126 2369 3011 Size: 274 80 33 51 1443 149 243 642 Cuts at: 3011 3436 5879 7591 96 Size: 425 2443 1712 138 Fragments arranged by size: 2443 1712 1443 642 425 274 243 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7593 7625 7593 Size: 32 7601 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7633 XbaI T'CTAG_A Cuts at: 4969 4969 Size: 7633 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7633 XmaI C'CCGG_G Cuts at: 1870 4958 1870 Size: 3088 4545 XmnI GAAnn'nnTTC Cuts at: 3696 3855 5762 3696 Size: 159 1907 5567 Fragments arranged by size: 5567 1907 159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5441 276 Size: 53 83 186 936 3907 2468 Fragments arranged by size: 3907 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI