################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AfaI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseRI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 150 TCCGGA T^CCGGA AcyI 31 GACGCC G[GA]^CG[CT]C AflIII 188 ACATGT A^C[GA][CT]GT AlwNI 429 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 144 AGCGCT AGC^GCT AspHI 61 GAGCAC G[AT]GC[AT]^C AvaII 153 GGACC G^G[AT]CC BmyI 61 GAGCAC G[AGT]GC[ACT]^C BpmI 46 CTGGAG CTGGAG(16/14) BsaHI 31 GACGCC G[GA]^CG[CT]C BsaJI 278 CCTTGG C^C[ACGT][ACGT]GG BsaWI 150 TCCGGA [AT]^CCGG[AT] BseAI 150 TCCGGA T^CCGGA BsiEI 71 CGGCCG CG[GA][CT]^CG BsiHKAI 61 GAGCAC G[AT]GC[AT]^C Bsp1286I 61 GAGCAC G[AGT]GC[ACT]^C BspEI 150 TCCGGA T^CCGGA BspHI 311 TCATGA T^CATGA BspLU11I 188 ACATGT A^CATGT BsrFI 110 ACCGGC [GA]^CCGG[CT] BssKI 291 CCAGG ^CC[ACGT]GG BstNI 291 CCAGG CC^[AT]GG BstYI 20 AGATCC [GA]^GATC[CT] Cfr10I 110 ACCGGC [GA]^CCGG[CT] Cfr13I 153 GGACC G^G[ACGT]CC EaeI 71 CGGCCG [CT]^GGCC[GA] EagI 71 CGGCCG C^GGCCG EarI 569 CTCTTC CTCTTC(1/4) EclXI 71 CGGCCG C^GGCCG Eco47III 144 AGCGCT AGC^GCT Eco52I 71 CGGCCG C^GGCCG EcoRII 291 CCAGG ^CC[AT]GG EcoT14I 278 CCTTGG C^C[AT][AT]GG EcoT22I 170 ATGCAT ATGCA^T HaeIII 72 GGCC GG^CC Hin1I 31 GACGCC G[GA]^CG[CT]C HphI 390 TCACC GGTGA(8/7) Ksp632I 569 CTCTTC CTCTTC(1/4) MaeIII 336 GTTAC ^GT[ACGT]AC MflI 20 AGATCC [GA]^GATC[CT] MroI 150 TCCGGA T^CCGGA MseI 343 TTAA T^TAA MslI 289 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 291 CCAGG CC^[AT]GG NotI 70 GCGGCCGC GC^GGCCGC NsiI 170 ATGCAT ATGCA^T NspI 188 ACATGT [GA]CATG^[CT] PstI 404 CTGCAG CTGCA^G RcaI 311 TCATGA T^CATGA Sau96I 153 GGACC G^G[ACGT]CC ScrFI 291 CCAGG CC^[ACGT]GG SfcI 404 CTGCAG C^T[GA][CT]AG SgrAI 109 CACCGGCG C[GA]^CCGG[CT]G StyI 278 CCTTGG C^C[AT][AT]GG Tru9I 343 TTAA T^TAA XcmI 35 CCAAGACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 20 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 21 GATCC GGATC(4/5) 90 GGATC BsgI 6 CTGCAC GTGCAG(16/14) 106 CTGCAC BsmFI 139 GGGAC GGGAC(10/14) 219 GTCCC CfoI 103 GCGC GCG^C 145 GCGC DpnII 21 GATC ^GATC 91 GATC HaeII 102 GGCGCT [GA]GCGC^[CT] 144 AGCGCT HapII 111 CCGG C^CGG 151 CCGG HgaI 31 GACGC GACGC(5/10) 39 GACGC HhaI 103 GCGC GCG^C 145 GCGC HinP1I 103 GCGC G^CGC 145 GCGC HpaII 111 CCGG C^CGG 151 CCGG MboI 21 GATC ^GATC 91 GATC MboII 18 GAAGA GAAGA(8/7) 570 TCTTC MspI 111 CCGG C^CGG 151 CCGG NdeII 21 GATC ^GATC 91 GATC NlaIII 189 CATG CATG^ 312 CATG Sau3AI 21 GATC ^GATC 91 GATC TaqI 14 TCGA T^CGA 540 TCGA TfiI 431 GAATC G^A[AT]TC 495 GATTC TspRI 561 CAGTG CA[GC]TG[ACGT][ACGT]^ 600 CACTG TthHB8I 14 TCGA T^CGA 540 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AciI AluI BbvI BsmAI BsrI BstF5I FauI Fnu4HI FokI HinfI ItaI MnlI MwoI PleI SfaNI TseI Tsp509I