Restriction map for
pFN21AE6365
(Circular) (Six-base) MAPSORT of: pFN21AE6365.seq Check: 6383 from: 1 to: 5980
pFN21AE6365 5980 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2292 2292
Size: 5980
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2313 3030 3323 1937
Size: 376 717 293 4594
Fragments arranged by size:
4594 717 376 293
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2126 2437 2522 3005 3183 4043 5427
Size: 433 61 311 85 483 178 860 1384
Cuts at: 5427 5695 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 860 483 433 311 268 178
85 61
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 2157 2265 4879 5856 1203
Size: 954 108 2614 977 1327
Fragments arranged by size:
2614 1327 977 954 108
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 3723 5295 3723
Size: 1572 4408
ApaLI G'TGCA_C
Cuts at: 2603 4037 5193 2603
Size: 1434 1156 3390
Fragments arranged by size:
3390 1434 1156
ApoI r'AATT_y
Cuts at: 1493 3289 3515 3667 5776 1493
Size: 1796 226 152 2109 1697
Fragments arranged by size:
2109 1796 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2598 2778 3305 1870
Size: 73 655 180 527 4545
Fragments arranged by size:
4545 655 527 180 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 4756 618
Size: 325 217 627 1514 1455 1842
Fragments arranged by size:
1842 1514 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 2382 3299 1391
Size: 684 307 917 4072
Fragments arranged by size:
4072 917 684 307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2689 928
Size: 971 790 4219
Fragments arranged by size:
4219 971 790
BbvCI CC'TCA_GC
Cuts at: 2178 2178
Size: 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4171 4205 1903
Size: 34 50 34 2150 34 3678
Fragments arranged by size:
3678 2150 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2507 3872 5082 2507
Size: 1365 1210 3405
Fragments arranged by size:
3405 1365 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BfrBI ATG'CAT
Cuts at: 2299 2299
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2559 5975 2559
Size: 3416 2564
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2607 2658 4041 5197 1790
Size: 817 51 1383 1156 2573
Fragments arranged by size:
2573 1383 1156 817 51
BmgBI CAC'GTC
Cuts at: 1318 2160 2268 1318
Size: 842 108 5030
Fragments arranged by size:
5030 842 108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2014 2032 2051 2153 2290 4640 1239
Size: 775 18 19 102 137 2350 2579
Fragments arranged by size:
2579 2350 775 137 102 19 18
Bpu10I CC'TnA_GC
Cuts at: 2178 2376 2178
Size: 198 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2897 4105 4970 5268 2897
Size: 1208 865 298 3609
Fragments arranged by size:
3609 1208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2468 2474 4643 882
Size: 617 969 6 2169 2219
Fragments arranged by size:
2219 2169 969 617 6
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 2924 5974 2924
Size: 3050 2930
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 3215 3245 1178
Size: 30 165 30 1296 30 486 30 3913
Fragments arranged by size:
3913 1296 486 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 1186
Size: 1492 4488
BseYI C'CCAG_C
Cuts at: 2075 2114 2173 5183 5679 2075
Size: 39 59 3010 496 2376
Fragments arranged by size:
3010 2376 496 59 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 1163
Size: 573 468 4939
Fragments arranged by size:
4939 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 3430 4191 5219 1540
Size: 275 186 141 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186 141
BsiHKAI G_wGCw'C
Cuts at: 2205 2382 2607 4041 4126 5197 2205
Size: 177 225 1434 85 1071 2988
Fragments arranged by size:
2988 1434 1071 225 177 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2205 2382 2607 2658 3299 4041
Size: 399 285 130 177 225 51 641 742
Cuts at: 4041 4126 5197 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 399 285 225 177
130 85 51
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 2871 3335 844
Size: 1448 579 464 3489
Fragments arranged by size:
3489 1448 579 464
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2329 4475 4649 65
Size: 1183 1081 2146 174 1396
Fragments arranged by size:
2146 1396 1183 1081 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2209 794
Size: 1415 4565
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2559 2941 3310 3677 4062 4079 5531 5617
Size: 587 382 369 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 587 385 382 369 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2933 3202 487
Size: 683 56 1707 269 3265
Fragments arranged by size:
3265 1707 683 269 56
BtsI GCAGTG_nn'
Cuts at: 2868 3156 3496 4290 4310 2868
Size: 288 340 794 20 4538
Fragments arranged by size:
4538 794 340 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2341 2376 529
Size: 35 1777 35 4133
Fragments arranged by size:
4133 1777 35 35
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 4987 809
Size: 507 1236 2435 1802
Fragments arranged by size:
2435 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2362 3427
Size: 54 1425 50 275 18 309 223 1065
Cuts at: 3427 4316 5945 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1065 889 309 275 223 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2139 3427 1537
Size: 275 327 1288 4090
Fragments arranged by size:
4090 1288 327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2044 3910 1353
Size: 691 1866 3423
Fragments arranged by size:
3423 1866 691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 2992 4567 4941 5087 387
Size: 1064 138 522 881 1575 374 146 1280
Fragments arranged by size:
1575 1280 1064 881 522 374 146 138
EcoICRI GAG'CTC
Cuts at: 2380 2380
Size: 5980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2014 2032 2051 2065 2126 2153 2290
Size: 393 382 18 19 14 61 27 137
Cuts at: 2290 2437 2522 3005 3183 4043 4640 5427 5695
Size: 147 85 483 178 860 597 787 268
Cuts at: 5695 1239
Size: 1524
Fragments arranged by size:
1524 860 787 597 483 393 382 268
178 147 137 85 61 27 19 18
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2759 2759
Size: 5980
EcoO109I rG'GnC_Cy
Cuts at: 2110 2110
Size: 5980
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
EcoRV GAT'ATC
Cuts at: 2170 2803 2170
Size: 633 5347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 3271 2467
Size: 804 5176
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2281 5127 1678
Size: 603 2846 2531
Fragments arranged by size:
2846 2531 603
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2402 2434 2699
Size: 32 135 32 662 32 136 32 265
Cuts at: 2699 2731 2887 2919 4621 4653 4695 4727 1373
Size: 32 156 32 1702 32 42 32 2626
Fragments arranged by size:
2626 1702 662 265 156 136 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2885 5094 5278 1305
Size: 1580 2209 184 2007
Fragments arranged by size:
2209 2007 1580 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2691 3938 4297 4456 518
Size: 648 1525 1247 359 159 2042
Fragments arranged by size:
2042 1525 1247 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2230 3396 4073 5221 5466 1606
Size: 571 53 1166 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 571 245 53
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5428
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NsiI A_TGCA'T
Cuts at: 2301 2301
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2303 3338 4883 1132
Size: 97 1074 1035 1545 2229
Fragments arranged by size:
2229 1545 1074 1035 97
PasI CC'CwG_GG
Cuts at: 1348 2122 1348
Size: 774 5206
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5078
PfoI T'CCnGG_A
Cuts at: 2130 2745 2130
Size: 615 5365
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 4049 4081 5562 5594 2831
Size: 32 1186 32 1481 32 3217
Fragments arranged by size:
3217 1481 1186 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2110
Size: 5980
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 2229 2595 2875 3332 830
Size: 1399 366 280 457 3478
Fragments arranged by size:
3478 1399 457 366 280
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2177 2177
Size: 5980
SacI G_AGCT'C
Cuts at: 2382 2382
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2225 2238 2591 2871 3328 4463 5144
Size: 219 1180 13 353 280 457 1135 681
Cuts at: 5144 5335 826
Size: 191 1471
Fragments arranged by size:
1471 1180 1135 681 457 353 280 219
191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2600 3307 1872
Size: 728 707 4545
Fragments arranged by size:
4545 728 707
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2912 4084 4985 5247 820
Size: 197 926 969 1172 901 262 1553
Fragments arranged by size:
1553 1172 969 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 2027 2765 3168 1517
Size: 510 738 403 4329
Fragments arranged by size:
4329 738 510 403
StyI C'CwwG_G
Cuts at: 513 1065 2055 2288 2523 3234 3419 513
Size: 552 990 233 235 711 185 3074
Fragments arranged by size:
3074 990 711 552 235 233 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2182 2492 4007 4024 4177 4362 4807
Size: 1255 602 310 1515 17 153 185 445
Cuts at: 4807 325
Size: 1498
Fragments arranged by size:
1515 1498 1255 602 445 310 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3007 3039 3215 3247 5940 5972 3007
Size: 32 176 32 2693 32 3015
Fragments arranged by size:
3015 2693 176 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 2598 3305 1870
Size: 728 707 4545
Fragments arranged by size:
4545 728 707
XmnI GAAnn'nnTTC
Cuts at: 3254 4109 3254
Size: 855 5125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI