(Linear) (Six-base) MAPSORT of: KIEE6365.seq Check: 863 from: 1 to: 1275 KIEE6365.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 287 1275 Size: 287 988 AccI GT'mk_AC Cuts at: 0 308 1025 1275 Size: 308 717 250 Fragments arranged by size: 717 308 250 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 121 432 517 1000 1178 1275 Size: 60 61 311 85 483 178 97 Fragments arranged by size: 483 311 178 97 85 61 60 AflIII A'CryG_T Cuts at: 0 152 260 1275 Size: 152 108 1015 Fragments arranged by size: 1015 152 108 ApaLI G'TGCA_C Cuts at: 0 598 1275 Size: 598 677 AvaI C'yCGr_G Cuts at: 0 593 773 1275 Size: 593 180 502 Fragments arranged by size: 593 502 180 BanII G_rGCy'C Cuts at: 0 70 377 1275 Size: 70 307 898 Fragments arranged by size: 898 307 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 684 1275 Size: 684 591 BbvCI CC'TCA_GC Cuts at: 0 173 1275 Size: 173 1102 BciVI GTATCCnnnnn_n' Cuts at: 0 502 1275 Size: 502 773 BfrBI ATG'CAT Cuts at: 0 294 1275 Size: 294 981 BglII A'GATC_T Cuts at: 0 554 1275 Size: 554 721 Bme1580I G_kGCm'C Cuts at: 0 602 653 1275 Size: 602 51 622 Fragments arranged by size: 622 602 51 BmgBI CAC'GTC Cuts at: 0 155 263 1275 Size: 155 108 1012 Fragments arranged by size: 1012 155 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 27 46 148 285 1275 Size: 9 18 19 102 137 990 Fragments arranged by size: 990 137 102 19 18 9 Bpu10I CC'TnA_GC Cuts at: 0 173 371 1275 Size: 173 198 904 Fragments arranged by size: 904 198 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 892 1275 Size: 892 383 BsaI GGTCTCn'nnnn_ Cuts at: 0 463 469 1275 Size: 463 6 806 Fragments arranged by size: 806 463 6 BsaBI GATnn'nnATC Cuts at: 0 919 1275 Size: 919 356 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 694 724 1210 1240 1275 Size: 694 30 486 30 35 Fragments arranged by size: 694 486 35 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 673 1275 Size: 673 602 BseYI C'CCAG_C Cuts at: 0 70 109 168 1275 Size: 70 39 59 1107 Fragments arranged by size: 1107 70 59 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 1275 Size: 199 1076 BsiEI CG_ry'CG Cuts at: 0 137 1275 Size: 137 1138 BsiHKAI G_wGCw'C Cuts at: 0 200 377 602 1275 Size: 200 177 225 673 Fragments arranged by size: 673 225 200 177 Bsp1286I G_dGCh'C Cuts at: 0 70 200 377 602 653 1275 Size: 70 130 177 225 51 622 Fragments arranged by size: 622 225 177 130 70 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 287 866 1275 Size: 287 579 409 Fragments arranged by size: 579 409 287 BsrDI GCAATG_nn' Cuts at: 0 324 1275 Size: 324 951 BstAPI GCAn_nnn'nTGC Cuts at: 0 204 1275 Size: 204 1071 BstXI CCAn_nnnn'nTGG Cuts at: 0 117 1275 Size: 117 1158 BstYI r'GATC_y Cuts at: 0 554 936 1275 Size: 554 382 339 Fragments arranged by size: 554 382 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 928 1197 1275 Size: 928 269 78 Fragments arranged by size: 928 269 78 BtsI GCAGTG_nn' Cuts at: 0 863 1151 1275 Size: 863 288 124 Fragments arranged by size: 863 288 124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 336 371 1275 Size: 336 35 904 Fragments arranged by size: 904 336 35 DrdI GACnn_nn'nnGTC Cuts at: 0 547 1275 Size: 547 728 EaeI y'GGCC_r Cuts at: 0 134 357 1275 Size: 134 223 918 Fragments arranged by size: 918 223 134 EagI C'GGCC_G Cuts at: 0 134 1275 Size: 134 1141 EarI CTCTTCn'nnn_ Cuts at: 0 39 1275 Size: 39 1236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 106 987 1275 Size: 106 881 288 Fragments arranged by size: 881 288 106 EcoICRI GAG'CTC Cuts at: 0 375 1275 Size: 375 900 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 27 46 60 121 148 285 432 Size: 9 18 19 14 61 27 137 147 Cuts at: 432 517 1000 1178 1275 Size: 85 483 178 97 Fragments arranged by size: 483 178 147 137 97 85 61 27 19 18 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 754 1275 Size: 754 521 EcoO109I rG'GnC_Cy Cuts at: 0 105 1275 Size: 105 1170 EcoRV GAT'ATC Cuts at: 0 165 798 1275 Size: 165 633 477 Fragments arranged by size: 633 477 165 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 462 1266 1275 Size: 462 804 9 Fragments arranged by size: 804 462 9 HaeII r_GCGC'y Cuts at: 0 276 1275 Size: 276 999 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 229 261 397 429 694 726 882 914 Size: 229 32 136 32 265 32 156 32 Cuts at: 914 1275 Size: 361 Fragments arranged by size: 361 265 229 156 136 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 880 1275 Size: 880 395 MslI CAynn'nnrTG Cuts at: 0 686 1275 Size: 686 589 MspA1I CmG'CkG Cuts at: 0 172 225 1275 Size: 172 53 1050 Fragments arranged by size: 1050 172 53 NsiI A_TGCA'T Cuts at: 0 296 1275 Size: 296 979 NspI r_CATG'y Cuts at: 0 298 1275 Size: 298 977 PasI CC'CwG_GG Cuts at: 0 117 1275 Size: 117 1158 PfoI T'CCnGG_A Cuts at: 0 125 740 1275 Size: 125 615 535 Fragments arranged by size: 615 535 125 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 826 858 1275 Size: 826 32 417 Fragments arranged by size: 826 417 32 PpuMI rG'GwC_Cy Cuts at: 0 105 1275 Size: 105 1170 PstI C_TGCA'G Cuts at: 0 224 590 870 1275 Size: 224 366 280 405 Fragments arranged by size: 405 366 280 224 PvuII CAG'CTG Cuts at: 0 172 1275 Size: 172 1103 SacI G_AGCT'C Cuts at: 0 377 1275 Size: 377 898 SfcI C'TryA_G Cuts at: 0 220 233 586 866 1275 Size: 220 13 353 280 409 Fragments arranged by size: 409 353 280 220 13 SmaI CCC'GGG Cuts at: 0 595 1275 Size: 595 680 SmlI C'TyrA_G Cuts at: 0 907 1275 Size: 907 368 StuI AGG'CCT Cuts at: 0 22 760 1163 1275 Size: 22 738 403 112 Fragments arranged by size: 738 403 112 22 StyI C'CwwG_G Cuts at: 0 50 283 518 1229 1275 Size: 50 233 235 711 46 Fragments arranged by size: 711 235 233 50 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 177 487 1275 Size: 177 310 788 Fragments arranged by size: 788 310 177 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1002 1034 1210 1242 1275 Size: 1002 32 176 32 33 Fragments arranged by size: 1002 176 33 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 724 1275 Size: 724 551 XmaI C'CCGG_G Cuts at: 0 593 1275 Size: 593 682 XmnI GAAnn'nnTTC Cuts at: 0 1249 1275 Size: 1249 26 Enzymes that do cut: AarI AccI AcuI AflIII ApaLI AvaI BanII BbsI BbvCI BciVI BfrBI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I MmeI MslI MspA1I NsiI NspI PasI PfoI PpiI PpuMI PstI PvuII SacI SfcI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BclI BglI BlpI BmrI BmtI BplI BsaAI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI ClaI DraI DraIII EcoRI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI