################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI Asp700I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BclI BcnI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 32 GGTACC G^GTACC AlwNI 1429 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1205 GGGCCC GGGCC^C Asp718I 32 GGTACC G^GTACC AspHI 1883 GTGCTC G[AT]GC[AT]^C AspI 707 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1528 GCTGAGG CCTCAGC(-5/-2) BbvI 1033 GCAGC GCAGC(8/12) BciVI 1837 GTATCC GTATCC(6/5) BglII 1535 AGATCT A^GATCT BsiHKAI 1883 GTGCTC G[AT]GC[AT]^C BsmBI 1803 GAGACG CGTCTC(1/5) BspMI 1154 ACCTGC ACCTGC(4/8) BsrDI 2018 GCAATG GCAATG(2/0) BsrFI 1175 GCCGGT [GA]^CCGG[CT] BstEII 279 GGTCACC G^GT[ACGT]ACC BstPI 279 GGTCACC G^GT[ACGT]ACC BstYI 1535 AGATCT [GA]^GATC[CT] Cfr10I 1175 GCCGGT [GA]^CCGG[CT] DsaI 205 CCATGG C^C[GA][CT]GG EcoO65I 279 GGTCACC G^GT[ACGT]ACC EcoRI 1290 GAATTC G^AATTC Fnu4HI 1033 GCAGC GC^[ACGT]GC HapII 1176 CCGG C^CGG HgaI 1977 GCGTC GACGC(5/10) HpaII 1176 CCGG C^CGG ItaI 1033 GCAGC GC^[ACGT]GC KpnI 32 GGTACC GGTAC^C MflI 1535 AGATCT [GA]^GATC[CT] MslI 1383 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1526 CAGCTG C[AC]G^C[GT]G MspI 1176 CCGG C^CGG NcoI 205 CCATGG C^CATGG PflFI 707 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 1526 CAGCTG CAG^CTG SalI 713 GTCGAC G^TCGAC SapI 1497 GAAGAGC GCTCTTC(1/4) ScaI 381 AGTACT AGT^ACT TseI 1033 GCAGC G^C[AT]GC Tth111I 707 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1051 TCTAGA T^CTAGA XcmI 36 CCAAAAGAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1535 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 438 GTCTAC GT^[AC][GT]AC 713 GTCGAC AciI 1116 CCGC CCGC(-3/-1) 993 GCGG AflII 1750 CTTAAG C^TTAAG 2059 CTTAAG AflIII 1088 ACATGT A^C[GA][CT]GT 1139 ACATGT AvaII 388 GGACC G^G[AT]CC 1232 GGACC BanII 1047 GGGCTC G[GA]GC[CT]^C 1205 GGGCCC BfrI 1750 CTTAAG C^TTAAG 2059 CTTAAG BspHI 1388 TCATGA T^CATGA 1637 TCATGA BspLU11I 1088 ACATGT A^CATGT 1139 ACATGT BssKI 1044 CCAGG ^CC[ACGT]GG 1712 CCAGG BstNI 1044 CCAGG CC^[AT]GG 1712 CCAGG DraII 886 GGGGCCT [GA]G^G[ACGT]CC[CT] 1204 GGGGCCC EcoO109I 886 GGGGCCT [GA]G^G[ACGT]CC[CT] 1204 GGGGCCC EcoRII 1044 CCAGG ^CC[AT]GG 1712 CCAGG EcoT14I 205 CCATGG C^C[AT][AT]GG 1896 CCAAGG FauI 1115 CCCGC CCCGC(4/6) 993 GCGGG MaeII 155 ACGT A^CGT 1239 ACGT MfeI 264 CAATTG C^AATTG 338 CAATTG MunI 264 CAATTG C^AATTG 338 CAATTG MvaI 1044 CCAGG CC^[AT]GG 1712 CCAGG NspI 1088 ACATGT [GA]CATG^[CT] 1139 ACATGT RcaI 1388 TCATGA T^CATGA 1637 TCATGA ScrFI 1044 CCAGG CC^[ACGT]GG 1712 CCAGG SspI 1765 AATATT AAT^ATT 2028 AATATT StyI 205 CCATGG C^C[AT][AT]GG 1896 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BanI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BstF5I Cfr13I DdeI DpnII EarI FokI HaeIII HincII HindII HinfI HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV PleI PshBI RsaI Sau3AI Sau96I SfaNI SmlI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I