Restriction map for
pFN21AB6255
(Circular) (Six-base) MAPSORT of: pFN21AB6255.seq Check: 135 from: 1 to: 6991
pFN21AB6255 6991 bp
With 182 enzymes: SgfI *
March 28, 2012 12:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4801 278
Size: 53 83 186 936 3265 2468
Fragments arranged by size:
3265 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2444 2719 4334 1937
Size: 507 275 1615 4594
Fragments arranged by size:
4594 1615 507 275
Acc65I G'GTAC_C
Cuts at: 2037 4312 2037
Size: 2275 4716
AclI AA'CG_TT
Cuts at: 1563 5118 5491 1563
Size: 3555 373 3063
Fragments arranged by size:
3555 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3028 3275 3682 5054 6438 6706 1632
Size: 1396 247 407 1372 1384 268 1917
Fragments arranged by size:
1917 1396 1384 1372 407 268 247
AflII C'TTAA_G
Cuts at: 820 1017 3755 4064 820
Size: 197 2738 309 3747
Fragments arranged by size:
3747 2738 309 197
AflIII A'CryG_T
Cuts at: 1203 3093 3144 5890 6867 1203
Size: 1890 51 2746 977 1327
Fragments arranged by size:
2746 1890 1327 977 51
AgeI A'CCGG_T
Cuts at: 5822 5822
Size: 6991
AhdI GACnn_n'nnGTC
Cuts at: 5720 5720
Size: 6991
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6991
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6991
AlwNI CAG_nnn'CTG
Cuts at: 3439 4734 6306 3439
Size: 1295 1572 4124
Fragments arranged by size:
4124 1572 1295
ApaI G_GGCC'C
Cuts at: 3214 3214
Size: 6991
ApaLI G'TGCA_C
Cuts at: 5048 6204 5048
Size: 1156 5835
ApoI r'AATT_y
Cuts at: 1493 2205 2759 3026 3295 4300 4526 4678 6787
Size: 712 554 267 269 1005 226 152 2109
Cuts at: 6787 1493
Size: 1697
Fragments arranged by size:
2109 1697 1005 712 554 269 267 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2645 3600 4053 5545 160
Size: 872 1613 955 453 1492 1606
Fragments arranged by size:
1613 1606 1492 955 872 453
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6991
AvaI C'yCGr_G
Cuts at: 1870 1943 4316 1870
Size: 73 2373 4545
Fragments arranged by size:
4545 2373 73
BamHI G'GATC_C
Cuts at: 4321 4688 4321
Size: 367 6624
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2946 3562 4312 5767
Size: 325 217 627 250 909 616 750 1455
Cuts at: 5767 618
Size: 1842
Fragments arranged by size:
1842 1455 909 750 627 616 325 250
217
BanII G_rGCy'C
Cuts at: 1391 3056 3214 4310 1391
Size: 1665 158 1096 4072
Fragments arranged by size:
4072 1665 1096 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6020
BbvCI CC'TCA_GC
Cuts at: 3534 3534
Size: 6991
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5182 5216 1903
Size: 34 3245 34 3678
Fragments arranged by size:
3678 3245 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3853 4883 6093 3853
Size: 1030 1210 4751
Fragments arranged by size:
4751 1210 1030
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6991
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5602 136
Size: 107 122 71 631 605 3930 1525
Fragments arranged by size:
3930 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3540 6986 3540
Size: 3446 3545
BlpI GC'TnA_GC
Cuts at: 4408 4408
Size: 6991
Bme1580I G_kGCm'C
Cuts at: 1790 3214 3565 5052 6208 1790
Size: 1424 351 1487 1156 2573
Fragments arranged by size:
2573 1487 1424 1156 351
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6991
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3946 4004 4722
Size: 416 53 83 399 24 2529 58 718
Cuts at: 4722 5680 442
Size: 958 1753
Fragments arranged by size:
2529 1753 958 718 416 399 83 58
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5651 1239
Size: 4412 2579
Bpu10I CC'TnA_GC
Cuts at: 3534 3891 3534
Size: 357 6634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2881 5116 5981 6279 2881
Size: 2235 865 298 3593
Fragments arranged by size:
3593 2235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5654 882
Size: 617 4155 2219
Fragments arranged by size:
4155 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4782 493
Size: 711 3578 2702
Fragments arranged by size:
3578 2702 711
BsaBI GATnn'nnATC
Cuts at: 6985 6985
Size: 6991
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4798 5180 5815 275
Size: 53 83 186 936 3265 382 635 1451
Fragments arranged by size:
3265 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5424 5822 6096 6243 6654 5424
Size: 398 274 147 411 5761
Fragments arranged by size:
5761 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2701 2731 1178
Size: 30 165 30 1298 30 5438
Fragments arranged by size:
5438 1298 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2536 2722 3551 1186
Size: 1350 186 829 4626
Fragments arranged by size:
4626 1350 829 186
BseYI C'CCAG_C
Cuts at: 3439 6194 6690 3439
Size: 2755 496 3740
Fragments arranged by size:
3740 2755 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6418
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4441 5202 6230 1540
Size: 275 186 2440 761 1028 2301
Fragments arranged by size:
2440 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3892 5052 5137 6208 3892
Size: 1160 85 1071 4675
Fragments arranged by size:
4675 1160 1071 85
BsmI GAATG_Cn'
Cuts at: 4502 4595 4502
Size: 93 6898
BsmBI CGTCTCn'nnnn_
Cuts at: 3802 3802
Size: 6991
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3056 3214 3565 3892 4310 5052 5137
Size: 399 1266 158 351 327 418 742 85
Cuts at: 5137 6208 1391
Size: 1071 2174
Fragments arranged by size:
2174 1266 1071 742 418 399 351 327
158 85
BspEI T'CCGG_A
Cuts at: 6654 6654
Size: 6991
BspHI T'CATG_A
Cuts at: 3393 3642 4880 6610 3393
Size: 249 1238 1730 3774
Fragments arranged by size:
3774 1730 1238 249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3168 4346 844
Size: 2324 1178 3489
Fragments arranged by size:
3489 2324 1178
BsrBI CCG'CTC
Cuts at: 1394 1823 4878 1394
Size: 429 3055 3507
Fragments arranged by size:
3507 3055 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4030 5486 5660 65
Size: 1183 2782 1456 174 1396
Fragments arranged by size:
2782 1456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3180 5635 5822 1700
Size: 110 1370 2455 187 2869
Fragments arranged by size:
2869 2455 1370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6991
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6991
BssSI C'ACGA_G
Cuts at: 5051 6063 5051
Size: 1012 5979
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6991
BstBI TT'CG_AA
Cuts at: 5795 5795
Size: 6991
BstEII G'GTnAC_C
Cuts at: 1184 2284 1184
Size: 1100 5891
BstYI r'GATC_y
Cuts at: 1972 3540 4321 4688 5073 5090 6542 6628 6640
Size: 1568 781 367 385 17 1452 86 12
Cuts at: 6640 6986 1972
Size: 346 1977
Fragments arranged by size:
1977 1568 1452 781 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2210 513
Size: 552 939 206 5294
Fragments arranged by size:
5294 939 552 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6252
Fragments arranged by size:
6252 683 56
BtsI GCAGTG_nn'
Cuts at: 3788 4507 5301 5321 3788
Size: 719 794 20 5458
Fragments arranged by size:
5458 794 719 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6991
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6956
DraI TTT'AAA
Cuts at: 4295 4295
Size: 6991
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5998 809
Size: 507 4682 1802
Fragments arranged by size:
4682 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4438 5327 6956
Size: 54 1425 50 275 18 2608 889 1629
Cuts at: 6956 8
Size: 43
Fragments arranged by size:
2608 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4438 1537
Size: 275 2626 4090
Fragments arranged by size:
4090 2626 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3097 3497 3701 4921 1353
Size: 1744 400 204 1220 3423
Fragments arranged by size:
3423 1744 1220 400 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5578 5952 6098 387
Size: 1064 138 3989 374 146 1280
Fragments arranged by size:
3989 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3028 3275 3682 5054 5651 6438 6706
Size: 393 1396 247 407 1372 597 787 268
Cuts at: 6706 1239
Size: 1524
Fragments arranged by size:
1524 1396 1372 787 597 407 393 268
247
EcoO109I rG'GnC_Cy
Cuts at: 2892 3210 2892
Size: 318 6673
EcoRI G'AATT_C
Cuts at: 3295 4300 4678 3295
Size: 1005 378 5608
Fragments arranged by size:
5608 1005 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 4115 2167
Size: 1948 5043
FspI TGC'GCA
Cuts at: 5497 5497
Size: 6991
HaeII r_GCGC'y
Cuts at: 1678 6138 1678
Size: 4460 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2701 2733 2813
Size: 32 135 32 443 32 654 32 80
Cuts at: 2813 2845 3167 3199 3532 3564 4171 4203 4235
Size: 32 322 32 333 32 607 32 32
Cuts at: 4235 5632 5664 5706 5738 1373
Size: 1397 32 42 32 2626
Fragments arranged by size:
2626 1397 654 607 443 333 322 135
80 42 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2181 2720 4335 4581 669
Size: 988 281 226 17 539 1615 246 3079
Fragments arranged by size:
3079 1615 988 539 281 246 226 17
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6991
HpaI GTT'AAC
Cuts at: 4581 4581
Size: 6991
KpnI G_GTAC'C
Cuts at: 2041 4316 2041
Size: 2275 4716
MfeI C'AATT_G
Cuts at: 2269 2343 4590 2269
Size: 74 2247 4670
Fragments arranged by size:
4670 2247 74
MluI A'CGCG_T
Cuts at: 6867 6867
Size: 6991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3354 6105 6289 1305
Size: 2049 2751 184 2007
Fragments arranged by size:
2751 2049 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5169
Fragments arranged by size:
5169 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3392 4949 5308 5467 518
Size: 648 2226 1557 359 159 2042
Fragments arranged by size:
2226 2042 1557 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3533 4407 5084 6232 6477 1606
Size: 1927 874 677 1148 245 2120
Fragments arranged by size:
2120 1927 1148 874 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2210 513
Size: 552 939 206 5294
Fragments arranged by size:
5294 939 552 206
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6991
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6991
NotI GC'GGCC_GC
Cuts at: 4438 4438
Size: 6991
NspI r_CATG'y
Cuts at: 1132 1229 3097 3148 4349 5894 1132
Size: 97 1868 51 1201 1545 2229
Fragments arranged by size:
2229 1868 1545 1201 97 51
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6991
PciI A'CATG_T
Cuts at: 3093 3144 5890 3093
Size: 51 2746 4194
Fragments arranged by size:
4194 2746 51
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6991
PmeI GTTT'AAAC
Cuts at: 4295 4295
Size: 6991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5060 5092 6573 6605 5060
Size: 32 1481 32 5446
Fragments arranged by size:
5446 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6991
PsiI TTA'TAA
Cuts at: 3933 4244 4561 3933
Size: 311 317 6363
Fragments arranged by size:
6363 317 311
PspOMI G'GGCC_C
Cuts at: 3210 3210
Size: 6991
PstI C_TGCA'G
Cuts at: 830 4343 830
Size: 3513 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6991
PvuII CAG'CTG
Cuts at: 3533 3533
Size: 6991
SalI G'TCGA_C
Cuts at: 1936 2718 4333 1936
Size: 782 1615 4594
Fragments arranged by size:
4594 1615 782
SapI GCTCTTCn'nnn_
Cuts at: 3497 3497
Size: 6991
SbfI CC_TGCA'GG
Cuts at: 4343 4343
Size: 6991
ScaI AGT'ACT
Cuts at: 2388 5239 6951 2388
Size: 2851 1712 2428
Fragments arranged by size:
2851 2428 1712
SfcI C'TryA_G
Cuts at: 826 1045 4339 5474 6155 6346 826
Size: 219 3294 1135 681 191 1471
Fragments arranged by size:
3294 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6991
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6991
SmaI CCC'GGG
Cuts at: 1872 4318 1872
Size: 2446 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2896 3755 4064 5095 5996 6258
Size: 197 926 953 859 309 1031 901 262
Cuts at: 6258 820
Size: 1553
Fragments arranged by size:
1553 1031 953 926 901 859 309 262
197
SnaBI TAC'GTA
Cuts at: 493 4782 493
Size: 4289 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6991
SphI G_CATG'C
Cuts at: 1132 4349 1132
Size: 3217 3774
SspI AAT'ATT
Cuts at: 5 52 3772 4035 4915 5
Size: 47 3720 263 880 2081
Fragments arranged by size:
3720 2081 880 263 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6991
StyI C'CwwG_G
Cuts at: 513 1065 2004 2210 3901 4430 513
Size: 552 939 206 1691 529 3074
Fragments arranged by size:
3074 1691 939 552 529 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5018 5035 5188 5373 5818 325
Size: 1255 3438 17 153 185 445 1498
Fragments arranged by size:
3438 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 5237 6949
Size: 274 80 33 51 1443 409 2851 1712
Cuts at: 6949 96
Size: 138
Fragments arranged by size:
2851 1712 1443 409 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3229 3261 6951 6983 3229
Size: 32 3690 32 3237
Fragments arranged by size:
3690 3237 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2715 1316
Size: 1399 5592
XbaI T'CTAG_A
Cuts at: 3056 4327 3056
Size: 1271 5720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 2048
Size: 6991
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6991
XmaI C'CCGG_G
Cuts at: 1870 4316 1870
Size: 2446 4545
XmnI GAAnn'nnTTC
Cuts at: 5120 5120
Size: 6991
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4799 276
Size: 53 83 186 936 3265 2468
Fragments arranged by size:
3265 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI