################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BbvCI BcgI BclI BfaI BfrI BlnI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KpnI KspI MaeI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI SexAI SfcI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 842 ACGTGT A^C[GA][CT]GT AviII 1700 TGCGCA TGC^GCA BbeI 850 GGCGCC GGCGC^C BciVI 1105 GGATAC GTATCC(6/5) BglI 1613 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1142 AGATCT A^GATCT BsaAI 559 TACGTG [CT]AC^GT[GA] BsaBI 1725 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 607 GAGACG CGTCTC(1/5) BsmI 1314 GAATGC GAATGC(1/-1) BsrBI 433 GAGCGG CCGCTC(-3/-3) BsrFI 60 GCCGGT [GA]^CCGG[CT] BssSI 1522 CACGAG CACGAG(-5/-1) BstBI 1311 TTCGAA TT^CGAA Cfr10I 60 GCCGGT [GA]^CCGG[CT] DraIII 690 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1347 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1316 ATGCAT ATGCA^T FspI 1700 TGCGCA TGC^GCA KasI 850 GGCGCC G^GCGCC MamI 1725 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1778 TTAA T^TAA MspA1I 273 CCGCTG C[AC]G^C[GT]G NarI 850 GGCGCC GG^CGCC NcoI 986 CCATGG C^CATGG NsiI 1316 ATGCAT ATGCA^T NspI 1706 GCATGC [GA]CATG^[CT] NspV 1311 TTCGAA TT^CGAA PflMI 47 CCATCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 257 GGGACCC [GA]G^G[AT]CC[CT] SacI 1714 GAGCTC GAGCT^C ScaI 242 AGTACT AGT^ACT SfoI 850 GGCGCC GGC^GCC SfuI 1311 TTCGAA TT^CGAA SmlI 1164 CTTGAG C^T[CT][GA]AG SphI 1706 GCATGC GCATG^C StuI 1620 AGGCCT AGG^CCT TfiI 1236 GAATC G^A[AT]TC Tru9I 1778 TTAA T^TAA Van91I 47 CCATCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 850 GGCGCC G[GA]^CG[CT]C 1171 GGCGTC AlwNI 913 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1193 CAGTCCCTG Asp700I 210 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1140 GAAGATCTTC BamHI 923 GGATCC G^GATCC 1286 GGATCC BlpI 486 GCTCAGC GC^T[ACGT]AGC 1716 GCTCAGC Bpu1102I 486 GCTCAGC GC^T[ACGT]AGC 1716 GCTCAGC BsaHI 850 GGCGCC G[GA]^CG[CT]C 1171 GGCGTC BsaI 1803 GAGACC GGTCTC(1/5) 1953 GGTCTC BsrDI 1182 CATTGC GCAATG(2/0) 1940 GCAATG CelII 486 GCTCAGC GC^T[ACGT]AGC 1716 GCTCAGC DsaI 713 CCGTGG C^C[GA][CT]GG 986 CCATGG EagI 5 CGGCCG C^GGCCG 92 CGGCCG EclXI 5 CGGCCG C^GGCCG 92 CGGCCG Eco52I 5 CGGCCG C^GGCCG 92 CGGCCG EcoT14I 236 CCAAGG C^C[AT][AT]GG 986 CCATGG Hin1I 850 GGCGCC G[GA]^CG[CT]C 1171 GGCGTC PleI 454 GACTC GAGTC(4/5) 472 GAGTC SapI 942 GAAGAGC GCTCTTC(1/4) 732 GCTCTTC SmaI 35 CCCGGG CCC^GGG 630 CCCGGG StyI 236 CCAAGG C^C[AT][AT]GG 986 CCATGG Tsp509I 1675 AATT ^AATT 1780 AATT XcmI 1091 CCATGACCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1283 CCAGGATCCGTGTGG XmaCI 35 CCCGGG C^CCGGG 630 CCCGGG XmaI 35 CCCGGG C^CCGGG 630 CCCGGG XmnI 210 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1140 GAAGATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AfaI AluI AlwI Aor51HI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco47III EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I XhoII