(Circular) (Six-base) MAPSORT of: pFN21AE4183.seq Check: 3934 from: 1 to: 6670 pFN21AE4183 6670 bp With 182 enzymes: SgfI * March 28, 2012 13:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3447 3647 3681 3447 Size: 200 34 6436 Fragments arranged by size: 6436 200 34 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2872 2966 4733 6117 6385 1632 Size: 1240 94 1767 1384 268 1917 Fragments arranged by size: 1917 1767 1384 1240 268 94 AfeI AGC'GCT Cuts at: 2183 2666 3870 2183 Size: 483 1204 4983 Fragments arranged by size: 4983 1204 483 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 2847 5569 6546 1203 Size: 1644 2722 977 1327 Fragments arranged by size: 2722 1644 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 2603 1166 Size: 1437 5233 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2666 1184 Size: 1482 5188 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 3575 3607 3917 3949 2204 Size: 32 1339 32 310 32 4925 Fragments arranged by size: 4925 1339 310 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2923 3203 4413 5985 2923 Size: 280 1210 1572 3608 Fragments arranged by size: 3608 1572 1210 280 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 3979 4205 4357 6466 1493 Size: 2486 226 152 2109 1697 Fragments arranged by size: 2486 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2267 2635 3995 1870 Size: 73 97 227 368 1360 4545 Fragments arranged by size: 4545 1360 368 227 97 73 BamHI G'GATC_C Cuts at: 2928 3291 4000 4367 2928 Size: 363 709 367 5231 Fragments arranged by size: 5231 709 367 363 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2617 2855 3124 3377 3991 Size: 325 217 627 830 238 269 253 614 Cuts at: 3991 5446 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 830 627 614 325 269 253 238 217 BanII G_rGCy'C Cuts at: 1391 3041 3351 3723 3989 1391 Size: 1650 310 372 266 4072 Fragments arranged by size: 4072 1650 372 310 266 BbeI G_GCGC'C Cuts at: 2859 2859 Size: 6670 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2548 2869 2878 3668 928 Size: 971 649 321 9 790 3930 Fragments arranged by size: 3930 971 790 649 321 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4861 4895 1903 Size: 34 2924 34 3678 Fragments arranged by size: 3678 2924 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3104 4562 5772 3104 Size: 1458 1210 4002 Fragments arranged by size: 4002 1458 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BfrBI ATG'CAT Cuts at: 3323 3323 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3624 5281 Size: 107 122 71 631 605 337 1615 1657 Cuts at: 5281 136 Size: 1525 Fragments arranged by size: 1657 1615 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3147 6665 3147 Size: 3518 3152 BlpI GC'TnA_GC Cuts at: 2492 3722 4087 2492 Size: 1230 365 5075 Fragments arranged by size: 5075 1230 365 Bme1580I G_kGCm'C Cuts at: 1790 3268 3380 4731 5887 1790 Size: 1478 112 1351 1156 2573 Fragments arranged by size: 2573 1478 1351 1156 112 BmgBI CAC'GTC Cuts at: 1318 2794 2848 1318 Size: 1476 54 5140 Fragments arranged by size: 5140 1476 54 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2975 3116 3474 Size: 416 53 83 399 24 1558 141 358 Cuts at: 3474 4401 5359 442 Size: 927 958 1753 Fragments arranged by size: 1753 1558 958 927 416 399 358 141 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2686 3055 3772 2353 Size: 333 369 717 5251 Fragments arranged by size: 5251 717 369 333 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2185 2198 3386 3760 5330 1239 Size: 946 13 1188 374 1570 2579 Fragments arranged by size: 2579 1570 1188 946 374 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3190 4795 5660 5958 3190 Size: 1605 865 298 3902 Fragments arranged by size: 3902 1605 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3802 3964 5333 882 Size: 617 2303 162 1369 2219 Fragments arranged by size: 2303 2219 1369 617 162 BsaAI yAC'GTr Cuts at: 493 1204 2566 4461 493 Size: 711 1362 1895 2702 Fragments arranged by size: 2702 1895 1362 711 BsaBI GATnn'nnATC Cuts at: 3734 6664 3734 Size: 2930 3740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2856 3177 4477 4859 Size: 53 83 186 936 1323 321 1300 382 Cuts at: 4859 5494 275 Size: 635 1451 Fragments arranged by size: 1451 1323 1300 936 635 382 321 186 83 53 BsaWI w'CCGG_w Cuts at: 5103 5501 5775 5922 6333 5103 Size: 398 274 147 411 5440 Fragments arranged by size: 5440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 2639 2669 3108 Size: 30 165 30 801 30 405 30 439 Cuts at: 3108 3138 1178 Size: 30 4710 Fragments arranged by size: 4710 801 439 405 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 3031 3341 3404 1186 Size: 1234 611 310 63 4452 Fragments arranged by size: 4452 1234 611 310 63 BseYI C'CCAG_C Cuts at: 2200 2750 3865 5873 6369 2200 Size: 550 1115 2008 496 2501 Fragments arranged by size: 2501 2008 1115 550 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 3314 3428 1163 Size: 573 561 1017 114 4405 Fragments arranged by size: 4405 1017 573 561 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2100 2260 4120 4881 5909 Size: 275 186 12 87 160 1860 761 1028 Cuts at: 5909 1540 Size: 2301 Fragments arranged by size: 2301 1860 1028 761 275 186 160 87 12 BsiHKAI G_wGCw'C Cuts at: 2289 3396 3723 4731 4816 5887 2289 Size: 1107 327 1008 85 1071 3072 Fragments arranged by size: 3072 1107 1071 1008 327 85 BsmI GAATG_Cn' Cuts at: 3325 4181 4274 3325 Size: 856 93 5721 Fragments arranged by size: 5721 856 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2606 2606 Size: 6670 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 3041 3268 3351 3380 3396 3723 Size: 399 499 752 227 83 29 16 327 Cuts at: 3723 3989 4731 4816 5887 1391 Size: 266 742 85 1071 2174 Fragments arranged by size: 2174 1071 752 742 499 399 327 266 227 85 83 29 16 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 2706 2778 2829 4559 6289 2706 Size: 72 51 1730 1730 3087 Fragments arranged by size: 3087 1730 1730 72 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3336 3447 3647 3681 3696 4025 844 Size: 2492 111 200 34 15 329 3489 Fragments arranged by size: 3489 2492 329 200 111 34 15 BsrBI CCG'CTC Cuts at: 1394 1823 2440 4557 1394 Size: 429 617 2117 3507 Fragments arranged by size: 3507 2117 617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3186 3952 5165 5339 65 Size: 1183 1938 766 1213 174 1396 Fragments arranged by size: 1938 1396 1213 1183 766 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 5314 5501 1700 Size: 110 255 3249 187 2869 Fragments arranged by size: 3249 2869 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 3527 4730 5742 3527 Size: 1203 1012 4455 Fragments arranged by size: 4455 1203 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6670 BstBI TT'CG_AA Cuts at: 3317 5474 3317 Size: 2157 4513 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstXI CCAn_nnnn'nTGG Cuts at: 2089 2545 2608 2089 Size: 456 63 6151 Fragments arranged by size: 6151 456 63 BstYI r'GATC_y Cuts at: 1972 2892 2928 3147 3291 4000 4367 4752 4769 Size: 920 36 219 144 709 367 385 17 Cuts at: 4769 6221 6307 6319 6665 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 920 709 385 367 346 219 144 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2718 2991 513 Size: 552 939 714 273 4192 Fragments arranged by size: 4192 939 714 552 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2566 487 Size: 683 56 1340 4591 Fragments arranged by size: 4591 1340 683 56 BtsI GCAGTG_nn' Cuts at: 3445 3751 4186 4980 5000 3445 Size: 306 435 794 20 5115 Fragments arranged by size: 5115 794 435 306 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2125 2160 529 Size: 35 1561 35 5039 Fragments arranged by size: 5039 1561 35 35 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 2700 2700 Size: 6670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2049 2070 Size: 54 1425 50 275 18 180 39 21 Cuts at: 2070 2097 2799 2994 3045 3300 3893 4117 5006 Size: 27 702 195 51 255 593 224 889 Cuts at: 5006 6635 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 702 593 275 255 224 195 180 54 51 50 43 39 27 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 2097 4117 1537 Size: 275 198 87 2020 4090 Fragments arranged by size: 4090 2020 275 198 87 EarI CTCTTCn'nnn_ Cuts at: 1353 2744 2942 2984 3381 4600 1353 Size: 1391 198 42 397 1219 3423 Fragments arranged by size: 3423 1391 1219 397 198 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2164 2489 3952 5257 5631 5777 Size: 1064 138 575 325 1463 1305 374 146 Cuts at: 5777 387 Size: 1280 Fragments arranged by size: 1463 1305 1280 1064 575 374 325 146 138 EcoICRI GAG'CTC Cuts at: 3721 3721 Size: 6670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2185 2198 2872 2966 3386 3760 4733 Size: 393 553 13 674 94 420 374 973 Cuts at: 4733 5330 6117 6385 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 973 787 674 597 553 420 393 374 268 94 13 EcoNI CCTnn'n_nnAGG Cuts at: 3356 3356 Size: 6670 EcoO109I rG'GnC_Cy Cuts at: 2263 2920 3914 2263 Size: 657 994 5019 Fragments arranged by size: 5019 994 657 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2266 2239 Size: 27 6643 FspI TGC'GCA Cuts at: 3707 5176 3707 Size: 1469 5201 HaeII r_GCGC'y Cuts at: 1678 2185 2668 2859 3365 3872 5817 1678 Size: 507 483 191 506 507 1945 2531 Fragments arranged by size: 2531 1945 507 507 506 483 191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2834 2866 3415 Size: 32 135 32 872 32 358 32 549 Cuts at: 3415 3447 5311 5343 5385 5417 1373 Size: 32 1864 32 42 32 2626 Fragments arranged by size: 2626 1864 872 549 358 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4014 4260 669 Size: 988 281 2076 246 3079 Fragments arranged by size: 3079 2076 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6670 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KasI G'GCGC_C Cuts at: 2855 2855 Size: 6670 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3240 5784 5968 1305 Size: 1935 2544 184 2007 Fragments arranged by size: 2544 2007 1935 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 2801 2996 3302 10 Size: 54 1425 343 240 729 195 306 3378 Fragments arranged by size: 3378 1425 729 343 306 240 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 2792 3017 4628 4987 5146 518 Size: 648 1437 189 225 1611 359 159 2042 Fragments arranged by size: 2042 1611 1437 648 359 225 189 159 MspA1I CmG'CkG Cuts at: 1606 2280 4086 4763 5911 6156 1606 Size: 674 1806 677 1148 245 2120 Fragments arranged by size: 2120 1806 1148 677 674 245 NarI GG'CG_CC Cuts at: 2856 2856 Size: 6670 NcoI C'CATG_G Cuts at: 513 1065 2004 2991 513 Size: 552 939 987 4192 Fragments arranged by size: 4192 987 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NsiI A_TGCA'T Cuts at: 3325 3325 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 3715 4028 5573 1132 Size: 97 2486 313 1545 2229 Fragments arranged by size: 2486 2229 1545 313 97 PasI CC'CwG_GG Cuts at: 1348 2917 1348 Size: 1569 5101 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 2058 1108 Size: 950 5720 PfoI T'CCnGG_A Cuts at: 2376 2376 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2204 2236 4739 4771 6252 6284 2204 Size: 32 2503 32 1481 32 2590 Fragments arranged by size: 2590 2503 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 2263 Size: 6670 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PstI C_TGCA'G Cuts at: 830 4022 830 Size: 3192 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 SacI G_AGCT'C Cuts at: 3723 3723 Size: 6670 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SanDI GG'GwC_CC Cuts at: 2263 2263 Size: 6670 SapI GCTCTTCn'nnn_ Cuts at: 2744 2942 2744 Size: 198 6472 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 2249 4918 6630 2249 Size: 2669 1712 2289 Fragments arranged by size: 2669 2289 1712 SfcI C'TryA_G Cuts at: 826 1045 4018 5153 5834 6025 826 Size: 219 2973 1135 681 191 1471 Fragments arranged by size: 2973 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2857 2857 Size: 6670 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 2042 2637 3997 1872 Size: 170 595 1360 4545 Fragments arranged by size: 4545 1360 595 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 3169 4774 5675 5937 820 Size: 197 926 1226 1605 901 262 1553 Fragments arranged by size: 1605 1553 1226 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 3715 4028 1132 Size: 2583 313 3774 Fragments arranged by size: 3774 2583 313 SrfI GCCC'GGGC Cuts at: 2042 2042 Size: 6670 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 3627 1517 Size: 2110 4560 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2991 3966 4109 513 Size: 552 939 237 750 975 143 3074 Fragments arranged by size: 3074 975 939 750 552 237 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2581 2810 3636 4697 4714 4867 5052 Size: 1255 1001 229 826 1061 17 153 185 Cuts at: 5052 5497 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1061 1001 826 445 229 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 2409 4916 Size: 274 80 33 51 1443 270 162 2507 Cuts at: 4916 6628 96 Size: 1712 138 Fragments arranged by size: 2507 1712 1443 274 270 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2639 2671 2684 2716 3108 3140 3586 3618 3913 Size: 32 13 32 392 32 446 32 295 Cuts at: 3913 3945 6630 6662 2639 Size: 32 2685 32 2647 Fragments arranged by size: 2685 2647 446 392 295 32 32 32 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3103 3295 3103 Size: 192 6478 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 2040 2635 3995 1870 Size: 170 595 1360 4545 Fragments arranged by size: 4545 1360 595 170 XmnI GAAnn'nnTTC Cuts at: 2219 3149 4799 2219 Size: 930 1650 4090 Fragments arranged by size: 4090 1650 930 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SwaI