(Linear) (Six-base) MAPSORT of: KIEE4183.seq Check: 8005 from: 1 to: 1965 KIEE4183.orf With 182 enzymes: SgfI * March 22, 2012 17:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1442 1642 1676 1965 Size: 1442 200 34 289 Fragments arranged by size: 1442 289 200 34 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 867 961 1965 Size: 867 94 1004 Fragments arranged by size: 1004 867 94 AfeI AGC'GCT Cuts at: 0 178 661 1865 1965 Size: 178 483 1204 100 Fragments arranged by size: 1204 483 178 100 AflIII A'CryG_T Cuts at: 0 842 1965 Size: 842 1123 AleI CACnn'nnGTG Cuts at: 0 598 1965 Size: 598 1367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 661 1965 Size: 661 1304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 199 231 1570 1602 1912 1944 1965 Size: 199 32 1339 32 310 32 21 Fragments arranged by size: 1339 310 199 32 32 32 21 AlwNI CAG_nnn'CTG Cuts at: 0 918 1198 1965 Size: 918 280 767 Fragments arranged by size: 918 767 280 AvaI C'yCGr_G Cuts at: 0 35 262 630 1965 Size: 35 227 368 1335 Fragments arranged by size: 1335 368 227 35 BamHI G'GATC_C Cuts at: 0 923 1286 1965 Size: 923 363 679 Fragments arranged by size: 923 679 363 BanI G'GyrC_C Cuts at: 0 612 850 1119 1372 1965 Size: 612 238 269 253 593 Fragments arranged by size: 612 593 269 253 238 BanII G_rGCy'C Cuts at: 0 1036 1346 1718 1965 Size: 1036 310 372 247 Fragments arranged by size: 1036 372 310 247 BbeI G_GCGC'C Cuts at: 0 854 1965 Size: 854 1111 BbsI GAAGACnn'nnnn_ Cuts at: 0 543 864 873 1663 1965 Size: 543 321 9 790 302 Fragments arranged by size: 790 543 321 302 9 BciVI GTATCCnnnnn_n' Cuts at: 0 1099 1965 Size: 1099 866 BfrBI ATG'CAT Cuts at: 0 1318 1965 Size: 1318 647 BglI GCCn_nnn'nGGC Cuts at: 0 1619 1965 Size: 1619 346 BglII A'GATC_T Cuts at: 0 1142 1965 Size: 1142 823 BlpI GC'TnA_GC Cuts at: 0 487 1717 1965 Size: 487 1230 248 Fragments arranged by size: 1230 487 248 Bme1580I G_kGCm'C Cuts at: 0 1263 1375 1965 Size: 1263 112 590 Fragments arranged by size: 1263 590 112 BmgBI CAC'GTC Cuts at: 0 789 843 1965 Size: 789 54 1122 Fragments arranged by size: 1122 789 54 BmrI ACTGGGnnnn_n' Cuts at: 0 970 1111 1469 1965 Size: 970 141 358 496 Fragments arranged by size: 970 496 358 141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 348 681 1050 1767 1965 Size: 348 333 369 717 198 Fragments arranged by size: 717 369 348 333 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 180 193 1381 1755 1965 Size: 180 13 1188 374 210 Fragments arranged by size: 1188 374 210 180 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1185 1965 Size: 1185 780 BsaI GGTCTCn'nnnn_ Cuts at: 0 1797 1959 1965 Size: 1797 162 6 Fragments arranged by size: 1797 162 6 BsaAI yAC'GTr Cuts at: 0 561 1965 Size: 561 1404 BsaBI GATnn'nnATC Cuts at: 0 1729 1965 Size: 1729 236 BsaHI Gr'CG_yC Cuts at: 0 851 1172 1965 Size: 851 321 793 Fragments arranged by size: 851 793 321 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 199 229 634 664 1103 1133 1965 Size: 199 30 405 30 439 30 832 Fragments arranged by size: 832 439 405 199 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 1026 1336 1399 1965 Size: 415 611 310 63 566 Fragments arranged by size: 611 566 415 310 63 BseYI C'CCAG_C Cuts at: 0 195 745 1860 1965 Size: 195 550 1115 105 Fragments arranged by size: 1115 550 195 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 1309 1423 1965 Size: 292 1017 114 542 Fragments arranged by size: 1017 542 292 114 BsiEI CG_ry'CG Cuts at: 0 8 95 255 1965 Size: 8 87 160 1710 Fragments arranged by size: 1710 160 87 8 BsiHKAI G_wGCw'C Cuts at: 0 284 1391 1718 1965 Size: 284 1107 327 247 Fragments arranged by size: 1107 327 284 247 BsmI GAATG_Cn' Cuts at: 0 1320 1965 Size: 1320 645 BsmBI CGTCTCn'nnnn_ Cuts at: 0 601 1965 Size: 601 1364 Bsp1286I G_dGCh'C Cuts at: 0 284 1036 1263 1346 1375 1391 1718 1965 Size: 284 752 227 83 29 16 327 247 Fragments arranged by size: 752 327 284 247 227 83 29 16 BspHI T'CATG_A Cuts at: 0 701 773 824 1965 Size: 701 72 51 1141 Fragments arranged by size: 1141 701 72 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1331 1442 1642 1676 1691 1965 Size: 1331 111 200 34 15 274 Fragments arranged by size: 1331 274 200 111 34 15 BsrBI CCG'CTC Cuts at: 0 435 1965 Size: 435 1530 BsrDI GCAATG_nn' Cuts at: 0 1181 1947 1965 Size: 1181 766 18 Fragments arranged by size: 1181 766 18 BsrFI r'CCGG_y Cuts at: 0 60 1965 Size: 60 1905 BssSI C'ACGA_G Cuts at: 0 1522 1965 Size: 1522 443 BstBI TT'CG_AA Cuts at: 0 1312 1965 Size: 1312 653 BstXI CCAn_nnnn'nTGG Cuts at: 0 84 540 603 1965 Size: 84 456 63 1362 Fragments arranged by size: 1362 456 84 63 BstYI r'GATC_y Cuts at: 0 887 923 1142 1286 1965 Size: 887 36 219 144 679 Fragments arranged by size: 887 679 219 144 36 BtgI C'CryG_G Cuts at: 0 713 986 1965 Size: 713 273 979 Fragments arranged by size: 979 713 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 561 1965 Size: 561 1404 BtsI GCAGTG_nn' Cuts at: 0 1440 1746 1965 Size: 1440 306 219 Fragments arranged by size: 1440 306 219 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 120 155 1965 Size: 120 35 1810 Fragments arranged by size: 1810 120 35 DraIII CAC_nnn'GTG Cuts at: 0 695 1965 Size: 695 1270 EaeI y'GGCC_r Cuts at: 0 5 44 65 92 794 989 1040 1295 Size: 5 39 21 27 702 195 51 255 Cuts at: 1295 1888 1965 Size: 593 77 Fragments arranged by size: 702 593 255 195 77 51 39 27 21 5 EagI C'GGCC_G Cuts at: 0 5 92 1965 Size: 5 87 1873 Fragments arranged by size: 1873 87 5 EarI CTCTTCn'nnn_ Cuts at: 0 739 937 979 1376 1965 Size: 739 198 42 397 589 Fragments arranged by size: 739 589 397 198 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 159 484 1947 1965 Size: 159 325 1463 18 Fragments arranged by size: 1463 325 159 18 EcoICRI GAG'CTC Cuts at: 0 1716 1965 Size: 1716 249 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 180 193 867 961 1381 1755 1965 Size: 180 13 674 94 420 374 210 Fragments arranged by size: 674 420 374 210 180 94 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 1351 1965 Size: 1351 614 EcoO109I rG'GnC_Cy Cuts at: 0 258 915 1909 1965 Size: 258 657 994 56 Fragments arranged by size: 994 657 258 56 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 234 261 1965 Size: 234 27 1704 Fragments arranged by size: 1704 234 27 FspI TGC'GCA Cuts at: 0 1702 1965 Size: 1702 263 HaeII r_GCGC'y Cuts at: 0 180 663 854 1360 1867 1965 Size: 180 483 191 506 507 98 Fragments arranged by size: 507 506 483 191 180 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 439 471 829 861 1410 1442 1965 Size: 439 32 358 32 549 32 523 Fragments arranged by size: 549 523 439 358 32 32 32 KasI G'GCGC_C Cuts at: 0 850 1965 Size: 850 1115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1235 1965 Size: 1235 730 MscI TGG'CCA Cuts at: 0 67 796 991 1297 1965 Size: 67 729 195 306 668 Fragments arranged by size: 729 668 306 195 67 MslI CAynn'nnrTG Cuts at: 0 598 787 1012 1965 Size: 598 189 225 953 Fragments arranged by size: 953 598 225 189 MspA1I CmG'CkG Cuts at: 0 275 1965 Size: 275 1690 NarI GG'CG_CC Cuts at: 0 851 1965 Size: 851 1114 NcoI C'CATG_G Cuts at: 0 986 1965 Size: 986 979 NsiI A_TGCA'T Cuts at: 0 1320 1965 Size: 1320 645 NspI r_CATG'y Cuts at: 0 1710 1965 Size: 1710 255 PasI CC'CwG_GG Cuts at: 0 912 1965 Size: 912 1053 PflMI CCAn_nnn'nTGG Cuts at: 0 53 1965 Size: 53 1912 PfoI T'CCnGG_A Cuts at: 0 371 1965 Size: 371 1594 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 199 231 1965 Size: 199 32 1734 Fragments arranged by size: 1734 199 32 PpuMI rG'GwC_Cy Cuts at: 0 258 1965 Size: 258 1707 SacI G_AGCT'C Cuts at: 0 1718 1965 Size: 1718 247 SanDI GG'GwC_CC Cuts at: 0 258 1965 Size: 258 1707 SapI GCTCTTCn'nnn_ Cuts at: 0 739 937 1965 Size: 739 198 1028 Fragments arranged by size: 1028 739 198 ScaI AGT'ACT Cuts at: 0 244 1965 Size: 244 1721 SfoI GGC'GCC Cuts at: 0 852 1965 Size: 852 1113 SmaI CCC'GGG Cuts at: 0 37 632 1965 Size: 37 595 1333 Fragments arranged by size: 1333 595 37 SmlI C'TyrA_G Cuts at: 0 1164 1965 Size: 1164 801 SphI G_CATG'C Cuts at: 0 1710 1965 Size: 1710 255 SrfI GCCC'GGGC Cuts at: 0 37 1965 Size: 37 1928 StuI AGG'CCT Cuts at: 0 1622 1965 Size: 1622 343 StyI C'CwwG_G Cuts at: 0 236 986 1965 Size: 236 750 979 Fragments arranged by size: 979 750 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 576 805 1631 1965 Size: 576 229 826 334 Fragments arranged by size: 826 576 334 229 TatI w'GTAC_w Cuts at: 0 242 404 1965 Size: 242 162 1561 Fragments arranged by size: 1561 242 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 634 666 679 711 1103 1135 1581 1613 Size: 634 32 13 32 392 32 446 32 Cuts at: 1613 1908 1940 1965 Size: 295 32 25 Fragments arranged by size: 634 446 392 295 32 32 32 32 32 25 13 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1098 1290 1965 Size: 1098 192 675 Fragments arranged by size: 1098 675 192 XmaI C'CCGG_G Cuts at: 0 35 630 1965 Size: 35 595 1335 Fragments arranged by size: 1335 595 35 XmnI GAAnn'nnTTC Cuts at: 0 214 1144 1965 Size: 214 930 821 Fragments arranged by size: 930 821 214 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI AvaI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PasI PflMI PfoI PpiI PpuMI SacI SanDI SapI ScaI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BclI BmtI Bpu10I BsaWI BsiWI BspEI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI DraI DrdI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SbfI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI ZraI