(Circular) (Six-base) MAPSORT of: pFN21AE6282.seq Check: 8837 from: 1 to: 5473 pFN21AE6282 5473 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3283 278 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 3536 4920 5188 1632 Size: 443 1461 1384 268 1917 Fragments arranged by size: 1917 1461 1384 443 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 4372 5349 1203 Size: 3169 977 1327 Fragments arranged by size: 3169 1327 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5473 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2415 1184 Size: 1231 4242 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2148 2180 2148 Size: 32 5441 AlwNI CAG_nnn'CTG Cuts at: 2479 3216 4788 2479 Size: 737 1572 3164 Fragments arranged by size: 3164 1572 737 ApaLI G'TGCA_C Cuts at: 3530 4686 3530 Size: 1156 4317 ApoI r'AATT_y Cuts at: 1493 2189 2782 3008 3160 5269 1493 Size: 696 593 226 152 2109 1697 Fragments arranged by size: 2109 1697 696 593 226 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2798 1870 Size: 73 70 785 4545 Fragments arranged by size: 4545 785 73 70 AvrII C'CTAG_G Cuts at: 2071 2071 Size: 5473 BamHI G'GATC_C Cuts at: 2803 3170 2803 Size: 367 5106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 4249 618 Size: 325 217 627 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 325 217 BanII G_rGCy'C Cuts at: 1391 2792 1391 Size: 1401 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3664 3698 1903 Size: 34 50 34 1643 34 3678 Fragments arranged by size: 3678 1643 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 4084 136 Size: 107 122 71 631 605 370 2042 1525 Fragments arranged by size: 2042 1525 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 3534 4690 1790 Size: 1744 1156 2573 Fragments arranged by size: 2573 1744 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 3204 4162 Size: 416 53 83 399 24 839 948 958 Cuts at: 4162 442 Size: 1753 Fragments arranged by size: 1753 958 948 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4133 1239 Size: 2894 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2739 3598 4463 4761 2739 Size: 859 865 298 3451 Fragments arranged by size: 3451 865 859 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 4136 882 Size: 617 792 1845 2219 Fragments arranged by size: 2219 1845 792 617 BsaAI yAC'GTr Cuts at: 493 1204 2671 3264 493 Size: 711 1467 593 2702 Fragments arranged by size: 2702 1467 711 593 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3280 3662 4297 275 Size: 53 83 186 936 1747 382 635 1451 Fragments arranged by size: 1747 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2148 2178 1178 Size: 30 165 30 745 30 4473 Fragments arranged by size: 4473 745 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5473 BseYI C'CCAG_C Cuts at: 4676 5172 4676 Size: 496 4977 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2633 1163 Size: 573 897 4003 Fragments arranged by size: 4003 897 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2923 3684 4712 1540 Size: 275 186 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3534 3619 4690 3534 Size: 85 1071 4317 Fragments arranged by size: 4317 1071 85 BsmI GAATG_Cn' Cuts at: 2065 2452 2984 3077 2065 Size: 387 532 93 4461 Fragments arranged by size: 4461 532 387 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2792 3534 3619 4690 1391 Size: 399 1002 742 85 1071 2174 Fragments arranged by size: 2174 1071 1002 742 399 85 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 844 Size: 1984 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3360 1394 Size: 429 1537 3507 Fragments arranged by size: 3507 1537 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 2721 3968 4142 65 Size: 1183 1275 198 1247 174 1396 Fragments arranged by size: 1396 1275 1247 1183 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4117 4304 1700 Size: 110 2307 187 2869 Fragments arranged by size: 2869 2307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 2372 2674 794 Size: 1578 302 3593 Fragments arranged by size: 3593 1578 302 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468 Size: 831 367 385 17 1452 86 12 346 Cuts at: 5468 1972 Size: 1977 Fragments arranged by size: 1977 1452 831 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2184 2184 Size: 5473 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4921 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4734 Fragments arranged by size: 4734 683 56 BtsI GCAGTG_nn' Cuts at: 2304 2989 3783 3803 2304 Size: 685 794 20 3974 Fragments arranged by size: 3974 794 685 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DraIII CAC_nnn'GTG Cuts at: 2674 2674 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 809 Size: 507 3164 1802 Fragments arranged by size: 3164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2920 3809 5438 Size: 54 1425 50 275 18 1090 889 1629 Cuts at: 5438 8 Size: 43 Fragments arranged by size: 1629 1425 1090 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2920 1537 Size: 275 1108 4090 Fragments arranged by size: 4090 1108 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2406 3403 1353 Size: 795 258 997 3423 Fragments arranged by size: 3423 997 795 258 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2017 4060 4434 4580 387 Size: 1064 138 428 2043 374 146 1280 Fragments arranged by size: 2043 1280 1064 428 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 3536 4133 4920 5188 1239 Size: 393 443 1461 597 787 268 1524 Fragments arranged by size: 1524 1461 787 597 443 393 268 EcoO109I rG'GnC_Cy Cuts at: 2100 2228 2100 Size: 128 5345 EcoRI G'AATT_C Cuts at: 2189 2782 3160 2189 Size: 593 378 4502 Fragments arranged by size: 4502 593 378 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 2494 4620 1678 Size: 816 2126 2531 Fragments arranged by size: 2531 2126 816 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373 Size: 32 135 32 2542 32 42 32 2626 Fragments arranged by size: 2626 2542 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2817 3063 669 Size: 988 281 879 246 3079 Fragments arranged by size: 3079 988 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 2451 2709 3072 2451 Size: 258 363 4852 Fragments arranged by size: 4852 363 258 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2458 4587 4771 1305 Size: 1153 2129 184 2007 Fragments arranged by size: 2129 2007 1153 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3651 Fragments arranged by size: 3651 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3431 3790 3949 518 Size: 648 1362 903 359 159 2042 Fragments arranged by size: 2042 1362 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2889 3566 4714 4959 1606 Size: 436 847 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 436 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4921 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2831 4376 1132 Size: 97 1602 1545 2229 Fragments arranged by size: 2229 1602 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5473 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PmlI CAC'GTG Cuts at: 2671 2671 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2148 2180 3542 3574 5055 5087 2148 Size: 32 1362 32 1481 32 2534 Fragments arranged by size: 2534 1481 1362 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2228 2100 Size: 128 5345 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PstI C_TGCA'G Cuts at: 830 2825 830 Size: 1995 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SbfI CC_TGCA'GG Cuts at: 2825 2825 Size: 5473 ScaI AGT'ACT Cuts at: 2319 3721 5433 2319 Size: 1402 1712 2359 Fragments arranged by size: 2359 1712 1402 SfcI C'TryA_G Cuts at: 826 1045 2821 3956 4637 4828 826 Size: 219 1776 1135 681 191 1471 Fragments arranged by size: 1776 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2015 2800 1872 Size: 143 785 4545 Fragments arranged by size: 4545 785 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2754 3577 4478 4740 820 Size: 197 926 811 823 901 262 1553 Fragments arranged by size: 1553 926 901 823 811 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5473 StyI C'CwwG_G Cuts at: 513 1065 2071 2912 513 Size: 552 1006 841 3074 Fragments arranged by size: 3074 1006 841 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 3500 3517 3670 3855 4300 325 Size: 1255 1067 853 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1067 853 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2236 2317 3719 Size: 274 80 33 51 1443 259 81 1402 Cuts at: 3719 5431 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1402 274 259 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2112 2144 5433 5465 2112 Size: 32 3289 32 2120 Fragments arranged by size: 3289 2120 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5473 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2013 2798 1870 Size: 143 785 4545 Fragments arranged by size: 4545 785 143 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3281 276 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI