Restriction map for
pFN21AE5255
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Van91I XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcyI 141 GGCGCC G[GA]^CG[CT]C
AlwI 471 GATCC GGATC(4/5)
AlwNI 58 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 351 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 141 GGCGCC G^G[CT][GA]CC
BanII 42 GAGCCC G[GA]GC[CT]^C
BbeI 141 GGCGCC GGCGC^C
BbsI 274 GAAGAC GAAGAC(2/6)
BclI 119 TGATCA T^GATCA
BlpI 403 GCTAAGC GC^T[ACGT]AGC
BpmI 371 CTGGAG CTGGAG(16/14)
Bpu1102I 403 GCTAAGC GC^T[ACGT]AGC
BpuAI 274 GAAGAC GAAGAC(2/6)
BsaHI 141 GGCGCC G[GA]^CG[CT]C
BsgI 11 GTGCAG GTGCAG(16/14)
BspMI 13 GCAGGT ACCTGC(4/8)
BsrBI 162 CCGCTC CCGCTC(-3/-3)
BsrI 92 CCAGT ACTGG(1/-1)
BssKI 7 CCAGG ^CC[ACGT]GG
BstF5I 245 GGATG GGATG(2/0)
BstNI 7 CCAGG CC^[AT]GG
CelII 403 GCTAAGC GC^T[ACGT]AGC
DraII 349 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 345 CCGCGG C^C[GA][CT]GG
EcoNI 51 CCTTCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 349 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 7 CCAGG ^CC[AT]GG
EcoRV 378 GATATC GAT^ATC
EcoT14I 257 CCTTGG C^C[AT][AT]GG
FbaI 119 TGATCA T^GATCA
FokI 245 GGATG GGATG(9/13)
HaeII 141 GGCGCC [GA]GCGC^[CT]
HapII 226 CCGG C^CGG
HgaI 168 GCGTC GACGC(5/10)
Hin1I 141 GGCGCC G[GA]^CG[CT]C
HpaII 226 CCGG C^CGG
KasI 141 GGCGCC G^GCGCC
KspI 345 CCGCGG CCGC^GG
MseI 332 TTAA T^TAA
MslI 489 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 226 CCGG C^CGG
MvaI 7 CCAGG CC^[AT]GG
NarI 141 GGCGCC GG^CGCC
NlaIII 367 CATG CATG^
NspI 366 GCATGC [GA]CATG^[CT]
PflFI 351 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 305 GAGTC GAGTC(4/5)
PpuMI 349 GGGACCC [GA]G^G[AT]CC[CT]
SacII 345 CCGCGG CCGC^GG
ScrFI 7 CCAGG CC^[ACGT]GG
SfaNI 488 GCATC GCATC(5/9)
SfoI 141 GGCGCC GGC^GCC
SphI 366 GCATGC GCATG^C
StuI 182 AGGCCT AGG^CCT
StyI 257 CCTTGG C^C[AT][AT]GG
TaqI 23 TCGA T^CGA
Tru9I 332 TTAA T^TAA
Tsp509I 124 AATT ^AATT
Tth111I 351 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 23 TCGA T^CGA
XbaI 444 TCTAGA T^CTAGA
XcmI 474 CCAGAAACACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AluI 402 AGCT AG^CT
407 AGCT
Alw44I 312 GTGCAC G^TGCAC
502 GTGCAC
ApaLI 312 GTGCAC G^TGCAC
502 GTGCAC
AspHI 312 GTGCAC G[AT]GC[AT]^C
502 GTGCAC
AvaII 152 GGTCC G^G[AT]CC
350 GGACC
BbvI 264 GCAGC GCAGC(8/12)
400 GCAGC
BfaI 388 CTAG C^TAG
445 CTAG
BsaJI 257 CCTTGG C^C[ACGT][ACGT]GG
345 CCGCGG
BsiHKAI 312 GTGCAC G[AT]GC[AT]^C
502 GTGCAC
BsmFI 349 GGGAC GGGAC(10/14)
130 GTCCC
BstEII 16 GGTCACC G^GT[ACGT]ACC
28 GGTCACC
BstPI 16 GGTCACC G^GT[ACGT]ACC
28 GGTCACC
Cfr13I 152 GGTCC G^G[ACGT]CC
350 GGACC
DpnII 120 GATC ^GATC
471 GATC
EcoO65I 16 GGTCACC G^GT[ACGT]ACC
28 GGTCACC
HaeIII 183 GGCC GG^CC
513 GGCC
HphI 18 TCACC GGTGA(8/7)
30 TCACC
MaeI 388 CTAG C^TAG
445 CTAG
MaeII 108 ACGT A^CGT
201 ACGT
MboI 120 GATC ^GATC
471 GATC
MboII 274 GAAGA GAAGA(8/7)
285 GAAGA
MspA1I 211 CCGCTG C[AC]G^C[GT]G
345 CCGCGG
NdeII 120 GATC ^GATC
471 GATC
Sau3AI 120 GATC ^GATC
471 GATC
Sau96I 152 GGTCC G^G[ACGT]CC
350 GGACC
TseI 264 GCAGC G^C[AT]GC
400 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AccII AciI BmyI BsiYI BslI
Bsp1286I BstUI Cac8I CfoI DdeI
FauI Fnu4HI HhaI HinP1I HinfI
ItaI MaeIII MnlI MvnI MwoI
NlaIV TfiI Tsp45I TspRI