################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AvrII BbvCI BcgI BciVI BfrI BglII BlnI BsiWI BsmBI Bsp1407I BspDI BspHI BsrDI BsrGI BssHII Bst1107I BstXI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I HindIII HpaI KpnI MfeI MunI NdeI NheI NotI NruI NsiI PacI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 1811 TCCGGA T^CCGGA BamHI 651 GGATCC G^GATCC BbeI 595 GGCGCC GGCGC^C BbrPI 1483 CACGTG CAC^GTG BbsI 304 GTCTTC GAAGAC(2/6) BclI 2 TGATCA T^GATCA BfaI 674 CTAG C^TAG BpuAI 304 GTCTTC GAAGAC(2/6) BsaBI 111 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1811 TCCGGA [AT]^CCGG[AT] BseAI 1811 TCCGGA T^CCGGA BsgI 343 CTGCAC GTGCAG(16/14) BsmI 1851 GCATTC GAATGC(1/-1) BspEI 1811 TCCGGA T^CCGGA BspMI 935 ACCTGC ACCTGC(4/8) BssSI 574 CACGAG CACGAG(-5/-1) BstBI 376 TTCGAA TT^CGAA BstEII 1293 GGTCACC G^GT[ACGT]ACC BstPI 1293 GGTCACC G^GT[ACGT]ACC EagI 1492 CGGCCG C^GGCCG EclXI 1492 CGGCCG C^GGCCG Eco52I 1492 CGGCCG C^GGCCG EcoO65I 1293 GGTCACC G^GT[ACGT]ACC FbaI 2 TGATCA T^GATCA FseI 214 GGCCGGCC GGCCGG^CC HincII 1414 GTCAAC GT[CT]^[GA]AC HindII 1414 GTCAAC GT[CT]^[GA]AC KasI 595 GGCGCC G^GCGCC MaeI 674 CTAG C^TAG MamI 111 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1101 ACGCGT A^CGCGT MroI 1811 TCCGGA T^CCGGA MseI 752 TTAA T^TAA NarI 595 GGCGCC GG^CGCC NspV 376 TTCGAA TT^CGAA PaeR7I 1736 CTCGAG C^TCGAG PmaCI 1483 CACGTG CAC^GTG PmlI 1483 CACGTG CAC^GTG PstI 556 CTGCAG CTGCA^G SacI 385 GAGCTC GAGCT^C SfoI 595 GGCGCC GGC^GCC SfuI 376 TTCGAA TT^CGAA SmlI 1736 CTCGAG C^T[CT][GA]AG StuI 746 AGGCCT AGG^CCT Tru9I 752 TTAA T^TAA XhoI 1736 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 805 GTGCAC G^TGCAC 1367 GTGCAC AlwNI 559 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 1456 CAGAAGCTG AocI 1119 CCTGAGG CC^T[ACGT]AGG 1858 CCTGAGG ApaLI 805 GTGCAC G^TGCAC 1367 GTGCAC AspI 922 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1335 GACGTGGTC BglI 187 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 460 GCCGAGGAGGC BsaAI 313 TACGTG [CT]AC^GT[GA] 1483 CACGTG BsiEI 759 CGACCG CG[GA][CT]^CG 1492 CGGCCG BspLU11I 67 ACATGT A^CATGT 1322 ACATGT BstYI 651 GGATCC [GA]^GATC[CT] 776 AGATCC Bsu36I 1119 CCTGAGG CC^T[ACGT]AGG 1858 CCTGAGG DraIII 1087 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG 1361 CACTGTGTG Eco81I 1119 CCTGAGG CC^T[ACGT]AGG 1858 CCTGAGG MflI 651 GGATCC [GA]^GATC[CT] 776 AGATCC NcoI 591 CCATGG C^CATGG 1445 CCATGG PflFI 922 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1335 GACGTGGTC PvuII 1501 CAGCTG CAG^CTG 1757 CAGCTG SapI 372 GCTCTTC GCTCTTC(1/4) 642 GCTCTTC SfcI 556 CTGCAG C^T[GA][CT]AG 1557 CTACAG SphI 1346 GCATGC GCATG^C 1867 GCATGC Tth111I 922 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1335 GACGTGGTC XcmI 722 CCACTTTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1298 CCATGACGACACTGG XhoII 651 GGATCC [GA]^GATC[CT] 776 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI ApaI AspHI AvaI AviII BalI BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XmaCI XmaI