Restriction map for
pFN21AE4175
(Linear) (Six-base) MAPSORT of: KIEE4175.seq Check: 1447 from: 1 to: 1905
KIEE4175.orf
With 182 enzymes: SgfI *
March 22, 2012 17:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1831 1905
Size: 94 1737 74
Fragments arranged by size:
1737 94 74
AflIII A'CryG_T
Cuts at: 0 67 359 1101 1322 1905
Size: 67 292 742 221 583
Fragments arranged by size:
742 583 292 221 67
AleI CACnn'nnGTG
Cuts at: 0 802 1905
Size: 802 1103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 1779 1905
Size: 175 1604 126
Fragments arranged by size:
1604 175 126
AlwNI CAG_nnn'CTG
Cuts at: 0 564 1461 1905
Size: 564 897 444
Fragments arranged by size:
897 564 444
ApaI G_GGCC'C
Cuts at: 0 518 571 1719 1905
Size: 518 53 1148 186
Fragments arranged by size:
1148 518 186 53
ApaLI G'TGCA_C
Cuts at: 0 805 1367 1905
Size: 805 562 538
Fragments arranged by size:
805 562 538
AvaI C'yCGr_G
Cuts at: 0 220 297 665 1235 1544 1580 1702 1736
Size: 220 77 368 570 309 36 122 34
Cuts at: 1736 1905
Size: 169
Fragments arranged by size:
570 368 309 220 169 122 77 36
34
BamHI G'GATC_C
Cuts at: 0 651 1905
Size: 651 1254
BanI G'GyrC_C
Cuts at: 0 293 595 1401 1905
Size: 293 302 806 504
Fragments arranged by size:
806 504 302 293
BanII G_rGCy'C
Cuts at: 0 389 490 518 571 836 1719 1905
Size: 389 101 28 53 265 883 186
Fragments arranged by size:
883 389 265 186 101 53 28
BbeI G_GCGC'C
Cuts at: 0 599 1905
Size: 599 1306
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 1905
Size: 297 1608
BclI T'GATC_A
Cuts at: 0 2 1905
Size: 2 1903
BglI GCCn_nnn'nGGC
Cuts at: 0 193 466 1905
Size: 193 273 1439
Fragments arranged by size:
1439 273 193
BlpI GC'TnA_GC
Cuts at: 0 991 1205 1774 1905
Size: 991 214 569 131
Fragments arranged by size:
991 569 214 131
Bme1580I G_kGCm'C
Cuts at: 0 518 571 809 1362 1371 1719 1905
Size: 518 53 238 553 9 348 186
Fragments arranged by size:
553 518 348 238 186 53 9
BmgBI CAC'GTC
Cuts at: 0 360 417 1023 1337 1905
Size: 360 57 606 314 568
Fragments arranged by size:
606 568 360 314 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 574 1318 1790 1905
Size: 574 744 472 115
Fragments arranged by size:
744 574 472 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1687 1905
Size: 1687 218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 901 1000 1525 1905
Size: 754 147 99 525 380
Fragments arranged by size:
754 525 380 147 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 217 403 717 730 1905
Size: 136 81 186 314 13 1175
Fragments arranged by size:
1175 314 186 136 81 13
BsaAI yAC'GTr
Cuts at: 0 315 1485 1905
Size: 315 1170 420
Fragments arranged by size:
1170 420 315
BsaBI GATnn'nnATC
Cuts at: 0 115 1905
Size: 115 1790
BsaHI Gr'CG_yC
Cuts at: 0 100 302 458 596 1905
Size: 100 202 156 138 1309
Fragments arranged by size:
1309 202 156 138 100
BsaWI w'CCGG_w
Cuts at: 0 1811 1905
Size: 1811 94
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 94 397 478 651 1546 1672 1680
Size: 12 82 303 81 173 895 126 8
Cuts at: 1680 1720 1905
Size: 40 185
Fragments arranged by size:
895 303 185 173 126 82 81 40
12 8
BseYI C'CCAG_C
Cuts at: 0 517 1026 1107 1882 1905
Size: 517 509 81 775 23
Fragments arranged by size:
775 517 509 81 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1905
Size: 328 1577
BsiEI CG_ry'CG
Cuts at: 0 762 1495 1905
Size: 762 733 410
Fragments arranged by size:
762 733 410
BsiHKAI G_wGCw'C
Cuts at: 0 320 389 809 1178 1371 1385 1905
Size: 320 69 420 369 193 14 520
Fragments arranged by size:
520 420 369 320 193 69 14
BsmI GAATG_Cn'
Cuts at: 0 1851 1905
Size: 1851 54
Bsp1286I G_dGCh'C
Cuts at: 0 320 389 490 518 571 809 836 1178
Size: 320 69 101 28 53 238 27 342
Cuts at: 1178 1362 1371 1385 1719 1905
Size: 184 9 14 334 186
Fragments arranged by size:
342 334 320 238 186 184 101 69
53 28 27 14 9
BspEI T'CCGG_A
Cuts at: 0 1811 1905
Size: 1811 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 944 1905
Size: 944 961
BsrBI CCG'CTC
Cuts at: 0 372 543 995 1905
Size: 372 171 452 910
Fragments arranged by size:
910 452 372 171
BsrFI r'CCGG_y
Cuts at: 0 215 334 443 529 1398 1494 1905
Size: 215 119 109 86 869 96 411
Fragments arranged by size:
869 411 215 119 109 96 86
BssSI C'ACGA_G
Cuts at: 0 574 1905
Size: 574 1331
BstAPI GCAn_nnn'nTGC
Cuts at: 0 902 1366 1461 1905
Size: 902 464 95 444
Fragments arranged by size:
902 464 444 95
BstBI TT'CG_AA
Cuts at: 0 377 1905
Size: 377 1528
BstEII G'GTnAC_C
Cuts at: 0 1293 1905
Size: 1293 612
BstYI r'GATC_y
Cuts at: 0 651 776 1905
Size: 651 125 1129
Fragments arranged by size:
1129 651 125
Bsu36I CC'TnA_GG
Cuts at: 0 1120 1859 1905
Size: 1120 739 46
Fragments arranged by size:
1120 739 46
BtgI C'CryG_G
Cuts at: 0 30 353 591 615 1445 1489 1905
Size: 30 323 238 24 830 44 416
Fragments arranged by size:
830 416 323 238 44 30 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1374 1646 1905
Size: 1374 272 259
Fragments arranged by size:
1374 272 259
BtsI GCAGTG_nn'
Cuts at: 0 345 1649 1905
Size: 345 1304 256
Fragments arranged by size:
1304 345 256
DraIII CAC_nnn'GTG
Cuts at: 0 1092 1366 1905
Size: 1092 274 539
Fragments arranged by size:
1092 539 274
EaeI y'GGCC_r
Cuts at: 0 86 194 959 1442 1448 1492 1905
Size: 86 108 765 483 6 44 413
Fragments arranged by size:
765 483 413 108 86 44 6
EagI C'GGCC_G
Cuts at: 0 1492 1905
Size: 1492 413
EarI CTCTTCn'nnn_
Cuts at: 0 374 379 649 703 1580 1905
Size: 374 5 270 54 877 325
Fragments arranged by size:
877 374 325 270 54 5
EcoICRI GAG'CTC
Cuts at: 0 387 1905
Size: 387 1518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 754 901 1000 1525 1831 1905
Size: 94 660 147 99 525 306 74
Fragments arranged by size:
660 525 306 147 99 94 74
EcoO109I rG'GnC_Cy
Cuts at: 0 514 568 1223 1716 1905
Size: 514 54 655 493 189
Fragments arranged by size:
655 514 493 189 54
FseI GG_CCGG'CC
Cuts at: 0 219 1905
Size: 219 1686
FspI TGC'GCA
Cuts at: 0 163 895 1057 1905
Size: 163 732 162 848
Fragments arranged by size:
848 732 163 162
FspAI rTGC'GCAy
Cuts at: 0 895 1057 1905
Size: 895 162 848
Fragments arranged by size:
895 848 162
HaeII r_GCGC'y
Cuts at: 0 472 599 643 1713 1905
Size: 472 127 44 1070 192
Fragments arranged by size:
1070 472 192 127 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1905
Size: 643 32 1230
Fragments arranged by size:
1230 643 32
HincII GTy'rAC
Cuts at: 0 1416 1905
Size: 1416 489
KasI G'GCGC_C
Cuts at: 0 595 1905
Size: 595 1310
MluI A'CGCG_T
Cuts at: 0 1101 1905
Size: 1101 804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1253 1900 1905
Size: 83 1170 647 5
Fragments arranged by size:
1170 647 83 5
MscI TGG'CCA
Cuts at: 0 88 961 1444 1450 1905
Size: 88 873 483 6 455
Fragments arranged by size:
873 483 455 88 6
MslI CAynn'nnrTG
Cuts at: 0 121 802 1393 1399 1905
Size: 121 681 591 6 506
Fragments arranged by size:
681 591 506 121 6
MspA1I CmG'CkG
Cuts at: 0 32 251 260 355 506 617 1503 1759
Size: 32 219 9 95 151 111 886 256
Cuts at: 1759 1905
Size: 146
Fragments arranged by size:
886 256 219 151 146 111 95 32
9
NaeI GCC'GGC
Cuts at: 0 217 531 1496 1905
Size: 217 314 965 409
Fragments arranged by size:
965 409 314 217
NarI GG'CG_CC
Cuts at: 0 596 1905
Size: 596 1309
NcoI C'CATG_G
Cuts at: 0 591 1445 1905
Size: 591 854 460
Fragments arranged by size:
854 591 460
NgoMIV G'CCGG_C
Cuts at: 0 215 529 1494 1905
Size: 215 314 965 411
Fragments arranged by size:
965 411 314 215
NspI r_CATG'y
Cuts at: 0 71 636 1326 1350 1431 1871 1905
Size: 71 565 690 24 81 440 34
Fragments arranged by size:
690 565 440 81 71 34 24
PasI CC'CwG_GG
Cuts at: 0 1167 1905
Size: 1167 738
PciI A'CATG_T
Cuts at: 0 67 1322 1905
Size: 67 1255 583
Fragments arranged by size:
1255 583 67
PflMI CCAn_nnn'nTGG
Cuts at: 0 1092 1337 1683 1882 1905
Size: 1092 245 346 199 23
Fragments arranged by size:
1092 346 245 199 23
PmlI CAC'GTG
Cuts at: 0 1485 1905
Size: 1485 420
PspOMI G'GGCC_C
Cuts at: 0 514 567 1715 1905
Size: 514 53 1148 190
Fragments arranged by size:
1148 514 190 53
PstI C_TGCA'G
Cuts at: 0 560 1905
Size: 560 1345
PvuII CAG'CTG
Cuts at: 0 1503 1759 1905
Size: 1503 256 146
Fragments arranged by size:
1503 256 146
SacI G_AGCT'C
Cuts at: 0 389 1905
Size: 389 1516
SacII CC_GC'GG
Cuts at: 0 33 356 618 1905
Size: 33 323 262 1287
Fragments arranged by size:
1287 323 262 33
SapI GCTCTTCn'nnn_
Cuts at: 0 379 649 1905
Size: 379 270 1256
Fragments arranged by size:
1256 379 270
SfcI C'TryA_G
Cuts at: 0 556 1557 1905
Size: 556 1001 348
Fragments arranged by size:
1001 556 348
SfoI GGC'GCC
Cuts at: 0 597 1905
Size: 597 1308
SmaI CCC'GGG
Cuts at: 0 299 667 1546 1582 1905
Size: 299 368 879 36 323
Fragments arranged by size:
879 368 323 299 36
SmlI C'TyrA_G
Cuts at: 0 1736 1905
Size: 1736 169
SphI G_CATG'C
Cuts at: 0 1350 1871 1905
Size: 1350 521 34
Fragments arranged by size:
1350 521 34
StuI AGG'CCT
Cuts at: 0 748 1905
Size: 748 1157
StyI C'CwwG_G
Cuts at: 0 89 591 1007 1445 1905
Size: 89 502 416 438 460
Fragments arranged by size:
502 460 438 416 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 427 1159 1527 1690 1905
Size: 241 186 732 368 163 215
Fragments arranged by size:
732 368 241 215 186 163
TatI w'GTAC_w
Cuts at: 0 1430 1517 1905
Size: 1430 87 388
Fragments arranged by size:
1430 388 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 869 901 1905
Size: 869 32 1004
Fragments arranged by size:
1004 869 32
Tth111I GACn'n_nGTC
Cuts at: 0 925 1338 1905
Size: 925 413 567
Fragments arranged by size:
925 567 413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 729 1305 1905
Size: 729 576 600
Fragments arranged by size:
729 600 576
XhoI C'TCGA_G
Cuts at: 0 1736 1905
Size: 1736 169
XmaI C'CCGG_G
Cuts at: 0 297 665 1544 1580 1905
Size: 297 368 879 36 325
Fragments arranged by size:
879 368 325 297 36
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FseI FspI FspAI
HaeII Hin4I HincII KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmlI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BfrBI BglII BmtI Bpu10I BpuEI
BsaXI BsiWI BsmBI BspHI BsrDI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraI DrdI EciI EcoNI EcoRI
EcoRV FalI HindIII HpaI KpnI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XmnI ZraI