Restriction map for
pFN21AE1714
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI MaeI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MspI MunI NaeI NarI NciI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp45I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 31 CGCG CG^CG
AcyI 526 GACGCC G[GA]^CG[CT]C
AlwNI 133 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 543 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI 327 GCAGC GCAGC(8/12)
BglII 578 AGATCT A^GATCT
BsaHI 526 GACGCC G[GA]^CG[CT]C
BseRI 274 GAGGAG GAGGAG(10/8)
BsmAI 265 GAGAC GTCTC(1/5)
BsrDI 237 CATTGC GCAATG(2/0)
BstAPI 335 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 31 CGCG CG^CG
BstYI 578 AGATCT [GA]^GATC[CT]
DdeI 414 CTCAG C^T[ACGT]AG
EaeI 433 CGGCCA [CT]^GGCC[GA]
EarI 271 GAAGAG CTCTTC(1/4)
Hin1I 526 GACGCC G[GA]^CG[CT]C
HphI 209 TCACC GGTGA(8/7)
Ksp632I 271 GAAGAG CTCTTC(1/4)
KspI 30 CCGCGG CCGC^GG
MflI 578 AGATCT [GA]^GATC[CT]
MvnI 31 CGCG CG^CG
NdeI 322 CATATG CA^TATG
PflMI 306 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 73 GACTC GAGTC(4/5)
PvuII 328 CAGCTG CAG^CTG
SacII 30 CCGCGG CCGC^GG
ScaI 377 AGTACT AGT^ACT
SmlI 75 CTCAAG C^T[CT][GA]AG
TfiI 282 GATTC G^A[AT]TC
TseI 327 GCAGC G^C[AT]GC
TspRI 133 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 306 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 578 AGATCT [GA]^GATC[CT]
XmnI 543 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 148 AAATTC [GA]^AATT[CT]
355 GAATTT
ApoI 148 AAATTC [GA]^AATT[CT]
355 GAATTT
BanII 197 GGGCTC G[GA]GC[CT]^C
511 GAGCCC
BmyI 197 GGGCTC G[AGT]GC[ACT]^C
511 GAGCCC
BsmFI 23 GGGAC GGGAC(10/14)
107 GGGAC
Bsp1286I 197 GGGCTC G[AGT]GC[ACT]^C
511 GAGCCC
BssKI 60 CCTGG ^CC[ACGT]GG
162 CCTGG
BstF5I 27 CATCC GGATG(2/0)
48 CATCC
BstNI 60 CCTGG CC^[AT]GG
162 CCTGG
BstXI 5 CCATGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
515 CCAAGTGCTTGG
Cfr13I 39 GGCCC G^G[ACGT]CC
159 GGGCC
EcoRII 60 CCTGG ^CC[AT]GG
162 CCTGG
EcoT14I 311 CCATGG C^C[AT][AT]GG
436 CCATGG
FokI 27 CATCC GGATG(9/13)
48 CATCC
HgaI 109 GACGC GACGC(5/10)
526 GACGC
HinfI 73 GACTC G^A[ACGT]TC
282 GATTC
MspA1I 30 CCGCGG C[AC]G^C[GT]G
328 CAGCTG
MvaI 60 CCTGG CC^[AT]GG
162 CCTGG
MwoI 335 GCATGATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
560 GCTTTGAGGGC
NcoI 311 CCATGG C^CATGG
436 CCATGG
Sau96I 39 GGCCC G^G[ACGT]CC
159 GGGCC
ScrFI 60 CCTGG CC^[ACGT]GG
162 CCTGG
StyI 311 CCATGG C^C[AT][AT]GG
436 CCATGG
Tsp509I 149 AATT ^AATT
356 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AfaI AluI BsaJI BsiYI
BslI BsrI Cac8I DpnII DsaI
FauI Fnu4HI HaeIII ItaI MboI
MboII MnlI NdeII NlaIII RsaI
Sau3AI