Restriction map for
pFN21AE4347
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BsaAI
BsaBI BsgI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI DsaI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 404 CGCG CG^CG
AcsI 700 AAATTT [GA]^AATT[CT]
AcyI 432 GACGCC G[GA]^CG[CT]C
ApaI 610 GGGCCC GGGCC^C
ApoI 700 AAATTT [GA]^AATT[CT]
AspHI 408 GAGCAC G[AT]GC[AT]^C
AvaII 1078 GGACC G^G[AT]CC
AvrII 195 CCTAGG C^CTAGG
BbsI 88 GAAGAC GAAGAC(2/6)
BfaI 196 CTAG C^TAG
BlnI 195 CCTAGG C^CTAGG
BpuAI 88 GAAGAC GAAGAC(2/6)
BsaHI 432 GACGCC G[GA]^CG[CT]C
BsiHKAI 408 GAGCAC G[AT]GC[AT]^C
BsmFI 1072 GTCCC GGGAC(10/14)
BsmI 866 GAATGC GAATGC(1/-1)
BsrBI 254 CCGCTC CCGCTC(-3/-3)
BsrFI 245 ACCGGC [GA]^CCGG[CT]
BstUI 404 CGCG CG^CG
CfoI 293 GCGC GCG^C
Cfr10I 245 ACCGGC [GA]^CCGG[CT]
DraIII 967 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 793 CGGCCG C^GGCCG
EarI 513 GAAGAG CTCTTC(1/4)
EclXI 793 CGGCCG C^GGCCG
Eco52I 793 CGGCCG C^GGCCG
HhaI 293 GCGC GCG^C
Hin1I 432 GACGCC G[GA]^CG[CT]C
HinP1I 293 GCGC G^CGC
Ksp632I 513 GAAGAG CTCTTC(1/4)
MaeI 196 CTAG C^TAG
MvnI 404 CGCG CG^CG
PpuMI 1077 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 580 CGATCG CGAT^CG
SbfI 276 CCTGCAGG CCTGCA^GG
Sse8387I 276 CCTGCAGG CCTGCA^GG
StuI 853 AGGCCT AGG^CCT
TfiI 919 GATTC G^A[AT]TC
Tsp509I 701 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 443 TCCGGA T^CCGGA
915 TCCGGA
BanII 473 GGGCTC G[GA]GC[CT]^C
610 GGGCCC
BlpI 189 GCTCAGC GC^T[ACGT]AGC
256 GCTCAGC
Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC
256 GCTCAGC
BsaWI 443 TCCGGA [AT]^CCGG[AT]
915 TCCGGA
BseAI 443 TCCGGA T^CCGGA
915 TCCGGA
BsiEI 580 CGATCG CG[GA][CT]^CG
793 CGGCCG
BspEI 443 TCCGGA T^CCGGA
915 TCCGGA
BspMI 168 ACCTGC ACCTGC(4/8)
377 ACCTGC
BstXI 242 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1058 CCAGGAAAGTGG
BstYI 227 AGATCC [GA]^GATC[CT]
742 AGATCC
CelII 189 GCTCAGC GC^T[ACGT]AGC
256 GCTCAGC
EcoNI 231 CCTCGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
895 CCTGGCAGAGG
EcoT14I 44 CCTTGG C^C[AT][AT]GG
195 CCTAGG
HgaI 402 GACGC GACGC(5/10)
432 GACGC
HincII 824 GTCAAC GT[CT]^[GA]AC
927 GTCAAC
HindII 824 GTCAAC GT[CT]^[GA]AC
927 GTCAAC
MflI 227 AGATCC [GA]^GATC[CT]
742 AGATCC
MroI 443 TCCGGA T^CCGGA
915 TCCGGA
MslI 93 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
142 CACCGAAGTG
MspA1I 259 CAGCTG C[AC]G^C[GT]G
806 CAGCTG
MwoI 475 GCTCTCCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
789 GCCCCGGCCGC
NlaIII 510 CATG CATG^
1026 CATG
PvuII 259 CAGCTG CAG^CTG
806 CAGCTG
SfaNI 547 GCATC GCATC(5/9)
562 GCATC
StyI 44 CCTTGG C^C[AT][AT]GG
195 CCTAGG
XhoII 227 AGATCC [GA]^GATC[CT]
742 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI BanI BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI NciI NdeII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I